Ramin Jahangirfard; Gholamreza Najafi; Ali Shalizar-Jalali; Abbas Ahmadi; Elham Zadehhashem
Volume 12, Issue 3 , September 2021, , Pages 333-338
Abstract
Ethephon (C2H6ClO3P; ETP), an organophosphorus pesticide regulating plant growth, is widely used for early ripening of fruits and vegetables in agriculture. The aim of this study was to investigate the effects of ETP on histomorphometrical and biochemical parameters in mouse testicular tissue. In this ...
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Ethephon (C2H6ClO3P; ETP), an organophosphorus pesticide regulating plant growth, is widely used for early ripening of fruits and vegetables in agriculture. The aim of this study was to investigate the effects of ETP on histomorphometrical and biochemical parameters in mouse testicular tissue. In this study, 90 adult male mice were randomly divided into six equal groups (n = 15). The ETP was administered orally at different doses (120, 240 and 480 mg kg-1) daily for 35 days. Untreated control, sham (received only normal saline) and neostigmine bromide-treated (positive control; 0.10 mg kg-1 orally; once per week) groups were also considered. Following 35 days, animals were euthanized and testicle and serum samples were taken. Accordingly, blood and serum acetylcholinesterase (AChE), catalase, total antioxidant capacity (TAC) and malondialdehyde (MDA) levels as well as histomorphometrical changes of testicles were investigated. The ETP-administered animals represented a significant reduction in AChE, TAC and catalase levels and remarkable increment in MDA content. A marked reduction was also seen in the germinal epithelium height, connective tissue thickness, seminiferous tubules diameter and Leydig cell number as well as spermiogenesis and Sertoli cell indices in ETP-treated mice compared to control ones. Similar findings were found in neostigmine bromide-treated animals. In conclusion, the ETP significantly affects the serum and blood anti-oxidant statuses and results in severe histological damages both at germ and somatic cell levels, suggesting its hematotoxic and reprotoxic characteristic.
Histology
Ramin Jahangirfard; Ali Shalizar; Rasoul Shahrooz; Gholamreza Najafi; Aram Minas
Volume 10, Issue 2 , June 2019, , Pages 159-163
Abstract
In order to conduct this study, eight adult turkey heads were obtained. Pituitary glands were harvested following cranial bones removal and examined morphologically and anatomically as well as topographically. Then, tissue sections were prepared and stained using Hematoxylin and Eosin, Alcian blue, orange ...
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In order to conduct this study, eight adult turkey heads were obtained. Pituitary glands were harvested following cranial bones removal and examined morphologically and anatomically as well as topographically. Then, tissue sections were prepared and stained using Hematoxylin and Eosin, Alcian blue, orange G and periodic acid-Schiff staining techniques. The results showed that turkey pituitary gland as a pea-sized structure is located in the ventral part of the cerebrum and composed of adenohypophysis and neurohypophysis parts. Moreover, histological analyses revealed that sinusoids are well-developed at the distal part of the adenohypophysis and irregular masses of endocrine cells exist among them. Distributions of basophilic cells in the distal part of adenohypophysis were significantly higher than those of other endocrine cells, while the acidophilic cells had the lowest distribution. Lower and higher numbers of chromophobe cells were also found compared to those of basophilic and acidophilic cells, respectively. These findings were mostly similar to the other birds’ pituitary gland anatomical and histological features, but there were also differences in cellular elements distributions along with infundibular cavity topography.
