Mahdi Eghbali; Sayed Mortaza Alavi-Shoushtari; Siamak Asri-Rezaei; Mohammad-Hassan Khadem Ansari
Volume 1, Issue 3 , December 2010, , Pages 142-148
Abstract
In order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. The semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption ...
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In order to determine iron and lead content of seminal plasma in water buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls. The semen characteristics were evaluated; its iron and lead content were estimated by atomic absorption spectrophotometer. The iron and lead content of the seminal plasma (Mean ± SEM) was recorded as 40.68 ± 0.75 mg L-1 and 0.026 ± 0.008 mg L -1, respectively. The mean iron value was highly associated with sperm progressive motility, gross motility and viability, negatively with lead content, and had a negative association with semen volume. The mean lead value was highly negatively associated with sperm progressive motility, gross motility, viability and positively associated with sperm abnormal morphology. For further clarification of these associations, the results were categorized in three groups of excellent (Ex, > 90 % motile, n = 33), good (Go, 80-89 % motile, n = 15) and moderate (Mo, < 79 % motile, n = 6) according to their percentage of sperm motility. The mean progressive motility in Ex, Go and Mo group was 92.24 ± 0.51 %, 81.66 ± 0.62 %, and 71.66 ± 1.05 % respectively. The mean iron and lead values and their associations with other parameters in these groups are discussed. The results show that seminal plasma iron content is associated with the motility and viability of the spermatozoa after ejaculation, but its lead content has an adverse effect on these parameters.
Clinical Pathology
Mahdi Eghbali; Sayed Mortaza Alavi-Shoushtari; Siamak Asri-Rezaei; Mohammad-Hassan Khadem Ansari
Volume 1, Issue 1 , June 2010, , Pages 12-20
Abstract
In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested ...
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In order to determine calcium (Ca), magnesium (Mg) content and total antioxidant capacity (TAC) of seminal plasma in buffalo and to study their associations with the semen characteristics, 54 semen samples were collected from 10 buffalo bulls; semen quality was evaluated, seminal plasma was then harvested by centrifugation and its Ca and Mg content were estimated and its TAC determined. The Ca and Mg content of the seminal plasma (Mean ± SEM) were recorded as 22.36 ± 0.52 mg dl-1 and 11.94 ± 0.36 mg dl-1 respectively, while, its mean TAC value was 1.50 ± 0.02 mmol L-1. The mean Ca value was highly associated with sperm progressive motility, gross motility, viability (P = 0.000 for all), negatively with semen volume (P = 0.01), and with Mg and TAC values (P = 0.000 for both). The mean Mg values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and TAC (P = 0.000 for all) and negatively associated with semen volume (P = 0.014). The mean TAC values was highly associated with sperm progressive motility, gross motility and viability and seminal plasma Ca and Mg (P = 0.000 for all). For further clarification of these associations, the data was categorized in three groups of excellent (Ex, >90% motile, n = 33), good (Go, 80-89% motile, n = 15) and moderate (Mo, Keywords: