Food Hygiene
Mobin Koohestani; Mehran Moradi; Hossein Tajik; Armen Badali
Volume 9, Issue 4 , December 2018, , Pages 301-306
Abstract
This study was carried out to investigate the stability, antibacterial properties and biofilm removal potential of cell-free supernatant (CFS) of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against Staphylococcus aureus ATCC 25923. Antibacterial activity of both Lactobacillus strains was ...
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This study was carried out to investigate the stability, antibacterial properties and biofilm removal potential of cell-free supernatant (CFS) of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against Staphylococcus aureus ATCC 25923. Antibacterial activity of both Lactobacillus strains was measured according to the agar spot method. The CFS was prepared by centrifugation of bacterial suspension at 4000 g for 10 min and the antimicrobial activity was measured using agar-well diffusion. The stability of CFSs during storage at 4.00 ± 2.00 °C and 25.00 ± 2.00 °C for a period of 4 weeks was measured based on the method of broth micro-dilution assay. Moreover, biofilm removal potential of CFS on 2-days-old biofilm of S. aureus developed on polystyrene and glass surfaces was also determined. The efficacy of CFS on bacterial biofilm established on the glass surface was also observed using fluorescence microscope. Results showed that inhibition zones of L. acidophilus (50.26 mm) were greater than L. casei (37.06 mm). The minimum inhibitory concentration of both CFSs remained stable (40 mg mL-1) during the storage for 28 days at 4.00 and 25.00 °C and storage temperature did not affect the antibacterial effectiveness of CFS. The addition of both CFSs significantly removed biofilm developed on both tested surfaces in a concentration-dependent manner. Biofilm removal property of L. acidophilus CFS was generally better than L. casei CFS which was confirmed by fluorescence microscope. The application of CFS of probiotic strains (i.e. Lactobacillus) as antibacterial and biofilm removal compounds could be very suitable to control the growth of food-borne pathogens.
Mosa Tavassoli; Farnaz Malekifard; Ali Soleimanzadeh; Hossein Tajik
Volume 2, Issue 4 , December 2011, , Pages 274-276
Abstract
This survey was carried out to determine the infection rate of Coenurus cerebralis in Urmia abattoir, West Azerbaijan, Iran. A total of 402 sheep heads were examined and the number of C. cerebralis cysts per each head was recorded. Thirty three sheep heads were examined each month during 12 months from ...
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This survey was carried out to determine the infection rate of Coenurus cerebralis in Urmia abattoir, West Azerbaijan, Iran. A total of 402 sheep heads were examined and the number of C. cerebralis cysts per each head was recorded. Thirty three sheep heads were examined each month during 12 months from 2006 to 2007. Of 402 heads, a number of 75 heads (18.65%) were infected with C. cerebralis. The cysts located in the left and right hemispheres and cerebellum were 54.63%, 40.20% and 5.15%, respectively. The infected heads contained 1-4 cysts. The highest and lowest prevalence were detected in sheep in March (42.42%) and in July (2.94%), respectively. The infection rates according to age of sheep were 42.02% in 0.5 to 2 years, 22.50% in 2 to 4 years and 8.92% in older than 4 years. The age specific infection rates among age groups were significantly differed (P < 0.05). Infection rate in Haraki breed (27.77%) was higher comparing to the rate in the Ghezel breed (21.13%) and Makoie breed (23.91%). However differences between sheep breeds were not statistically significant (P > 0.05).
Mosa Tavassoli; Abbas Imani; Mohammad Yousefnia Pasha; Amir Tukmechi; Hossein Tajik
Volume 1, Issue 2 , September 2010, , Pages 123-127
Abstract
This study was performed from February to April 2006; several visits were made to abattoirs in the north of Iran for Hypoderma spp infestation. Necropsy inspection of slaughtered and skinned animals were carried out by examination of the inner skin surface and subcutaneous tissues. Warbles were isolated ...
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This study was performed from February to April 2006; several visits were made to abattoirs in the north of Iran for Hypoderma spp infestation. Necropsy inspection of slaughtered and skinned animals were carried out by examination of the inner skin surface and subcutaneous tissues. Warbles were isolated by squeezing nodules from subcutaneous tissues. In the case of abscess presence, aseptic sample were taken from abscesses. The parasitological and bacteriological examinations were performed on the samples. The results indicated that 104 out of 958 of slaughtered animals were infested to Hypoderma spp in which 48 (46.15 %), 34 (32.69 %) and 22 (21.15 %) were infested to Hypoderma bovis, Hypoderma lineatum and both species, respectively. Following bacterial analysis, the following bacteria were isolated: Escherichia coli, Streptococcus pyogenes, Staphylococcus aureus, Staphylococcus epidermidis and Klebsiella pneumonia.
