Microbiology
Masood Adibhesami; Malahat Ahmadi; Amir Abbas Farshid; Farshid Sarrafzadeh-Rezaei; Bahram Dalir-Naghadeh
Volume 8, Issue 1 , March 2017, , Pages 23-28
Abstract
The microorganisms have been noted as the main cause of delayed wound healing.The most common pathogen causing the wound infections is Staphylococcus aureus. Silver nanoparticles (AgNPs) show ample antibacterial activities. In the present study, the effect of AgNPs on mouse wounds inoculated with S. ...
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The microorganisms have been noted as the main cause of delayed wound healing.The most common pathogen causing the wound infections is Staphylococcus aureus. Silver nanoparticles (AgNPs) show ample antibacterial activities. In the present study, the effect of AgNPs on mouse wounds inoculated with S. aureus was investigated. Sixty male mice (20 to 30 g) were anesthetized, full-thickness skin wounds were made on their back and then the bacterial suspension was added to each wound bed. Treatments were administered on wound bed topically including gentamicin (8 mg kg-1), AgNPs (0.08 mg kg-1, 0.04 mg kg-1 and 0.02 mg kg-1) and normal saline in the control group. Wound healing was monitored macroscopically by taking digital photographs on days 0, 7, 14 and 21 of the experiment. Topical application of gentamicin and AgNPs (0.08 and 0.04 mg kg-1) significantly increased the rate of wound healing more than treatment with AgNPs at a dose of 0.02 mg kg-1and normal saline. The presence of silver nanoparticles in AgNPs groups (especially 0.08 mg kg-1) improved wound appearance better than other groups without silver nanoparticles (gentamicin and control groups) and led to lesser wound scars. According to data analysis, healing rate of treated mice with gentamicin and AgNPs (0.08 mg kg-1) was significantly (p < 0.001) faster than treated mice with other AgNPs doses and normal saline. The results of current study introduced an in vivo nanosilver accelerating effects on the treatment of on S. aureus infected skin wounds.
Mahdi Dilmaghani; Malahat Ahmadi; Taghi Zahraei-Salehi; Alireza Talebi
Volume 2, Issue 3 , September 2011, , Pages 157-165
Abstract
Salmonella enterica serovar Typhimurium and S.enterica serovar Enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. According to their antigenic profiles, salmonella shows different disease syndromes and host specificities. It is necessary and important ...
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Salmonella enterica serovar Typhimurium and S.enterica serovar Enteritidis are the most frequently isolated serovars from food-borne diseases throughout the world. According to their antigenic profiles, salmonella shows different disease syndromes and host specificities. It is necessary and important to discriminate salmonella serovars from each other in order to ensure that each pathogen and its epidemiology are correctly recognized. Many PCR-based methods have been developed to identify salmonella serovars. The objective of present study was to identify S. Typhimurium in avians from different regions including: North, Northwest and capital city (Tehran) of Iran. Also in this research, the quality of CHROMagar™ Salmonella medium (CAS medium) in veterinary medicine was evaluated. The results of present study showed that out of 1870 intestine samples, fifty two S. Typhimurium including broiler (n=13), layer (n=12), duck (n=5), goose (n=5), sparrow (n=8), canary (n=3), pigeon (n=5) and African grey parrot (n=1) were identified using serotyping as well as multiplex-PCR. In conclusion, important measures must be taken on prevention and propagation of S. Typhimurium among avians. CHROMagar™ Salmonella medium has high levels of sensitivity and specificity and reduced the time to final identification of salmonella spp. in comparison with biochemical tests.
