Ehsanollah Sakhaee; Homayoon Babaei; Jalil Abshenas; Omid Azari; Reza Kheirandish; Farideh Ebrahimi-Meymand
Volume 2, Issue 4 , December 2011, , Pages 232-237
Abstract
In this study we investigated histopathological changes of mice ovarian tissues following long-term administration of copper sulfate and induction of chronic copper poisoning. The study comprised of three different groups of twenty one mice as follows: The first group (Group 100) which treated by gavage ...
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In this study we investigated histopathological changes of mice ovarian tissues following long-term administration of copper sulfate and induction of chronic copper poisoning. The study comprised of three different groups of twenty one mice as follows: The first group (Group 100) which treated by gavage with copper sulfate at a dose of 100 mg kg-1 for 5 weeks. The second group (Group 200), which received 200 mg kg-1 copper sulfate by gavage during experimental period (35 days), and control animals (Group C), which received the same volume of distilled water in the same way. The blood samples were obtained from 7 cases out of 21 animals of each group by cardiac puncture at the end of first, third and fifth week. Ovarian lesions were observed in group 100 after 35th day and in group 200 after 21st day. The histopathologic examination revealed widespread ovarian follicle atresia in group 200 after 35th day Atretic follicles had numerous cell debris and inflammatory cells in the antral cavity. In degenerative antral follicles granulose cells degenerated and desquamated into antrum. In some degenerative antral follicles infiltration of inflammatory cells into granulose cell layer and antrum were observed. The histopathlogic data indicate the possibility of adverse effect of copper poisoning on the mice ovarian tissue. Copper might be mediator of the effect of oxidative damage and play an essential role in reproductive system.
Jalil Abshenas; Homayoon Babaei; Mohammad-Hossein Zare; Asie Allahbakhshi; Fariba Sharififar
Volume 2, Issue 4 , December 2011, , Pages 242-247
Abstract
The objective of this study was to investigate whether or not the adverse effects of heat on sperm quality could be prevented by green tea extract (GTE) administration. Ninety adult male mice were randomly divided to two groups. The scrotum of each animal in the first group was immersed once for 20 min ...
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The objective of this study was to investigate whether or not the adverse effects of heat on sperm quality could be prevented by green tea extract (GTE) administration. Ninety adult male mice were randomly divided to two groups. The scrotum of each animal in the first group was immersed once for 20 min in a water bath maintained at 42 oC (heat group, H) and the second group (control group, C) was maintained at 23oC. Heat-treated and control groups were allocated randomly into three subgroups. The first subgroup from heat-treated mice was administered sterile saline (heat saline, HS) and the two other subgroups were administered orally with two different doses of GTE including 500 and 750 mg kg-1 (HG500 and HG750) for 49 consecutive days. Likewise, the first subgroup from control mice was administered sterile saline (control saline, CS) and the two other subgroups were administered orally with 500 and 750 mg kg-1 of GTE (CG500 and CG750), respectively. Heat stress significantly reduced (P < 0.05) sperm concentration, total sperm motility, progressive sperm motility and hypo-osmotic swelling-water test positive spermatozoa at the first 14 days after the heat treatment. However, a partial recovery was observed at the day 42, which was still significantly lower than that of the CS group. Administration of GTE in two doses (HG500 and HG750 treatment groups) following heat treatment could significantly recover adverse effects of heat on above-mentioned parameters during the first 28 days. The present study demonstrates that the adverse effects of hyperthermia on semen parameters might be recovered following administration of green tea extract in a short period of time.