Theriogenology
Abolfazl Hajibemani; Abdolah Mirzaei; Abbas Rowshan Ghasrodashti; Mohammad Reza Memarzadeh
Volume 7, Issue 4 , December 2016, , Pages 309-315
Abstract
In the present study, the effect of intrauterine infusion of Zataria multiflora extract on the clinical endometritis was investigated. Vaginal examination, transrectal palpation and ultrasonography were used to inspect the genital tract at 30-40 days in milk and two weeks later the same approach was ...
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In the present study, the effect of intrauterine infusion of Zataria multiflora extract on the clinical endometritis was investigated. Vaginal examination, transrectal palpation and ultrasonography were used to inspect the genital tract at 30-40 days in milk and two weeks later the same approach was applied. Cows with clinical endometritis were randomly divided into three treatment groups: Z. multiflora extract (n = 56), penicillin + streptomycin (pen + strep, n = 55), and placebo (n = 20). Cervical cytology, reagent strip test and cell counting by means of Neubauer hemocytometer were carried out in both examinations. Clinical cure rate of cows with endometritis of score 1 were 45.5, 34.5 and 53.6% in placebo, pen + strep and Z. multiflora, respectively. Clinical cure rate of cows with endometritis of score 2, 3 were 66.7, 84.6 and 56.0% in placebo, pen + strep and Z. multiflora, respectively. Overall, proportions of successfully treated cows were 55.0, 58.2 and 54.7% in placebo, pen + strep and Z. multiflora, respectively (p > 0.05). In placebo, none of the parameters were significantly different between first and second examination, while we found the significant differences in percentage of neutrophils and leukocyte esterase activity in other groups (p < 0.05). First service conception rate of cows was higher in Z. multiflora compared to other groups; however, this difference was not significant. In conclusion, pen + strep and Z. multiflora extract can be effective on the clinical endometritis and may improve reproductive performance. The extract of Z. multiflora can be useful as an alternative therapy for treatment of clinical endometritis in lactating dairy cows.
Mohammad Arjmand; Abdolah Mirzaei; Mohammad Reza Jafarzadeh Shirazi; Amin Tamadon; Mohammad Saied Salehi; Mehdi Saeb; Mohammad Reza Namavar; Mohammad Reza Zandi; Hojatollah Shahheidari; Sara Moradi
Volume 5, Issue 4 , December 2014, , Pages 247-254
Abstract
The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 ...
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The aims of the present study were to evaluate luteal activity in Abadeh ecotype goat during summer and winter and also the effect of a single dose kisspeptin-10 injection on the secretion of luteinizing hormone (LH) in female anestrous goats. In the first study, progesterone (P4) concentration in 10 goats in summer (n = 6) and winter (n = 4) were measured every other day. Moreover, in summer group, a male teaser goat was left in the herd on days of sampling for one hour. Goats with P4 concentration ≥1 ng mL-1, at least two consecutive measurements, were considered with luteal activity. In the second study, the anestrous phase was confirmed by P4 measurement 20 and 10 days before the kisspeptin injection in five female Abadeh ecotype goats (4 to 5 years old). The goats were given a single IV injection of saline (2 mL) as control group and the same goats (1 hr after the last blood sampling) were given kisspeptin (1 μg kg-1) as treatment group. The blood samples were collected at –60, –40, –20 and 0 min (before injection), and 10, 20, 30, 40, 50, 60, 80, 100, 120 and 140 min after the injection and LH concentration were measured. A single IV injection of 1 µg kg-1 of kisspeptin-10 did not stimulate the release of LH in female anestrous goats. In summer, in the presence of teaser goat, luteal activity was seen in all goats. In the absence of male goat in winter, some goats showed luteal activity and others showed anestrus.
Maede Moosavi; Abdolah Mirzaei; Mohsen Ghavami; Amin Tamadon
Volume 5, Issue 1 , March 2014, , Pages 13-19
Abstract
The aim of the present study was to compare the occurrence and duration of clinical mastitis in different seasons, stages of lactation period and parities in a Holstein dairy farm in Iran. A retrospective epidemiological survey from April 2005 to March 2008 was conducted on 884 clinical mastitis cases ...
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The aim of the present study was to compare the occurrence and duration of clinical mastitis in different seasons, stages of lactation period and parities in a Holstein dairy farm in Iran. A retrospective epidemiological survey from April 2005 to March 2008 was conducted on 884 clinical mastitis cases of 7437 lactations. Data of each case including calendar-date of mastitis onset, days in milk (DIM) of mastitis onset (early: 0-74 DIM; middle: 75-150 DIM, and late ≥ 150 DIM), duration of mastitis, and parity (1, 2, and ≥ 3) were recorded. Based on date of mastitis onset, cases were classified into stages of lactation. Moreover, beginning of mastitis was seasonally categorized. Duration of clinical mastitis after treatment in early lactation was less than late lactation in the first-parity cows (p = 0.005). In early lactation period, the first-parity cows suffered clinical mastitis in days earlier than two other parity groups (p < 0.001). Moreover, in late lactation period, the first-parity cows had clinical mastitis in days later than cows in the third and more parities (p = 0.002). Occurrence of clinical mastitis in summer increased in late lactation period but in winter increased in early lactation period (p = 0.001). In addition, occurrence time of clinical mastitis in summer were in days later than in spring (p = 0.02) and winter (p = 0.03) in early lactation period. In conclusion, occurrence of mastitis in winter and spring during early lactation and in summer during late lactation period were more prevalent especially in lower parities.
Abolfazl Hajibemani; Hassan Sharifiyazdi; Abdolah Mirzaei; Abbas Rowshan Ghasrodashti
Volume 3, Issue 1 , March 2012, , Pages 37-43
Abstract
Uterine infection is one of the reproductive diseases that can have disturbing postpartum uterine health in cattle. Therefore, identification of resistant genotypes to uterine infection is important. Lactoferrin (LF) is one of the major antimicrobial compounds in the normal uterine discharges of cows. ...
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Uterine infection is one of the reproductive diseases that can have disturbing postpartum uterine health in cattle. Therefore, identification of resistant genotypes to uterine infection is important. Lactoferrin (LF) is one of the major antimicrobial compounds in the normal uterine discharges of cows. We hypothesized that allelic diversity in LF gene may contribute to susceptibility or resistance to uterine infection. We investigated the single nucleotide polymorphism genes identified in the 5' untranslated region (5'-UTR, position +32) of the LF gene using Allele-specific PCR method in cows with and without uterine infection. Blood samples were collected from 89 multiparous Holstein dairy cows with a history of uterine infection (n = 51), and cows without disease as the control group (n= 38). The results indicated the presence of different proportion of polymorphisms (G > C) in the 5'-UTR area of cows in the all groups. The results of Allele specific PCR was in complete agreement with sequencing method. Statistical analysis did not show any statistically significant correlation between disease and SNP in 5'-UTR. While, there was a significant difference in the mean of reproductive parameters of cows without polymorphism compare to those of with SNP in 5'-UTR. Cows with +32:CC genotype and +32:GC genotype (cows with SNP in UTR) had lower average of services per conception and days open compared to cows with the +32:GG genotypes. However, no significant difference in the calving to first service was found between these genotypes. Further studies will be required to determine critical SNPs in LF gene and status of the risk of uterine infection and embryo survival in cows.