Poultry
Manoochehr Allymehr; Kamyar Yousefi; Alireza Talebi; Amir Tukmechi
Articles in Press, Accepted Manuscript, Available Online from 20 February 2024
Abstract
The aims of current investigation were to study the growth performance, carcass traits, meat quality and expression profile of Myostatin (MSTN), Insulin-like growth hormone type I (IGF-I), Myogenin (MyoG) and Myogenic regulatory factor 4 (MRF4) genes in three commercial broiler strains including Ross ...
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The aims of current investigation were to study the growth performance, carcass traits, meat quality and expression profile of Myostatin (MSTN), Insulin-like growth hormone type I (IGF-I), Myogenin (MyoG) and Myogenic regulatory factor 4 (MRF4) genes in three commercial broiler strains including Ross 308 (Ross), Cobb 500 (Cobb), and Arian (Arian) in 2023. A total of 240 one-day-old chicks were reared under an equalized standard management condition for six weeks. Performance, organ weights, meat quality, and the expression level of the myogenic genes in the pectoral muscle were investigated. The lowest body weight, feed intake, weight gain and highest feed conversion ratio (FCR) was observed for Arian at the end of the study (p<0.05). The meat quality was similar between strains (p>0.05). The IGF-I expression level was significantly higher at 42 days of age in Cobb compared to Ross and Arian (p<0.001). The MRF4 expression level was significantly higher at 28 days of age in Cobb compared to Ross (p<0.001). The MyoG expression level was significantly lower in Arian compared to Cobb at 42 days of age (p<0.05). Furthermore, the MSTN expression level was significantly lower in Cobb compared to Ross and Arian at 42 days of age (p<0.05). The remarkable differences in gene expression levels at the end of the rearing period was supported by higher growth performance and body weight of Cobb compared to RossandArian strains. In conclusion, the finding of current study will conveniently help to assess the performance of these broiler strains under similar rearing condition.
Microbiology
Seyed Sajjad Babaeimarzangou; Manoochehr Allymehr; Aidin Molouki; Alireza Talebi; Mohammad Hossein Fallah Mehrabadi
Volume 14, Issue 8 , August 2023, , Pages 447-456
Abstract
Newcastle disease virus (NDV) is considered one of the most devastating avian viral patho-gens affecting the avian population, and it causes a significant economic burden on the poultry industry worldwide. The study aimed to gain deeper understanding of the molecular and phylogenetic analyses of the ...
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Newcastle disease virus (NDV) is considered one of the most devastating avian viral patho-gens affecting the avian population, and it causes a significant economic burden on the poultry industry worldwide. The study aimed to gain deeper understanding of the molecular and phylogenetic analyses of the complete hemagglutinin-neuraminidase (HN) coding region among NDV isolates. The samples were obtained from different parts of Iran from July 2017 to February 2020, were used for phylogenic analysis in this study. The results confirmed the predominance of sub-genotype VII.1.1, previously known as sub-genotype VIIL, which is circulating in commercial broiler farms of Iran. Identification of (a) an additional N-glycosylation site (NIS) at position 144; (b) mutations S315P and I369V which are related to increasing the viral thermostability; (C) cysteine residues at positions 123; (d) amino acid substitutions in the HN antigenic sites, especially the mutations I514V and E347Q, as well as the other mutant within HN binding sites of the VII.1.1 sub-genotype, suggests the idea that this new sub-genotype of NDV may possess a high level of pathogenicity and virulence compared to other NDV sub-genotypes. In conclusion, the results indicate the presence of an additional NIS at position 144, which may alter the virulence of the isolates. Furthermore, the presence of the thermostable mutations (S315P and I369V) and the other amino acid substitutions among the VII.1.1 sub-genotype isolates may have an impact on the vaccine immunity against this new NDV sub-genotype.
Mohammad Hossein Alavi; Manoochehr Allymehr; Alireza Talebi; Gholamreza Najafi
Volume 11, Issue 2 , June 2020, , Pages 135-141
Abstract
It is well documented that aging has negative effects on fertility. With increasing age, the activity of antioxidant enzymes are reduced and because of roosters sperm composition, a high proportion of polyunsaturated fatty acids (PUFAs), the probability of sperm damage increases. The objective of the ...
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It is well documented that aging has negative effects on fertility. With increasing age, the activity of antioxidant enzymes are reduced and because of roosters sperm composition, a high proportion of polyunsaturated fatty acids (PUFAs), the probability of sperm damage increases. The objective of the present study was to compare the effects of nano-selenium and sodium selenite on fertility in aged male broiler breeder chickens. Thirty-five male broiler breeders (Cobb 500)® at 50 weeks of age were randomly divided into five equal groups: The control group was fed on a commercial diet, group T1 was fed on a commercial diet supplemented with sodium selenite (0.30 mg kg-1 feed), group T2, T3 and T4 were fed on a commercial diet supplemented with nano-selenium (0.15, 0.30 and 0.60 mg kg-1 feed, respectively). Sperm characteristics (sperm count, motility, viability, and maturity) as well as testicular histomorphometric features [tubule differentiation (TDI), spermiation (SPI), Sertoli cell (SCI) and miotic (MI) indices] were assessed. The results showed that sperm characteristics were gradually decreased with age in the control group, however, it increased in group T3. Also, TDI, SPI, SCI, and MI in group T3 were higher than those of other groups. Our findings revealed that dietary supplementations with nano-selenium boosted fertility in aged male broiler breeders and the best results were obtained when the roosters received 0.30 mg kg-1 nano-selenium. Supplementation of nano-selenium in aged broiler breeder males might be effective to maintain flock fertility and/or increase the flock fertility.
Poultry
Seyed Sattar Jalali; Alireza Talebi; Manoochehr Allymer; Ali Soleimanzadeh; Mazdak Razi
Volume 10, Issue 2 , June 2019, , Pages 139-144
Abstract
Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects ...
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Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogonial stem cells (SSCs) in testis of broiler breeder males. A total of 30 roosters of 40 weeks age were randomly divided into 5 groups. Groups were as follow: 1) control (normal diet), 2) diet supplemented with 0.3 mg/kg sodium Selenite, 3) diet supplemented with 0.15 mg/kg Nano-Se, 4) diet supplemented with 0.3 mg/kg Nano-Se and 5) diet supplemented with 0.6 mg/kg Nano-Se. At the end of experimental period, birds autopsied and samples from testis of all birds were taken. The samples were used to examine the β1-integrin (CD29), thy-1(CD90 and NANOG mRNA expression by quantitative Real-Time PCR. The results of this study showed that testis of the groups fed with diets supplemented with 0.6mg/kg and 0.15mg/kg of Nano-Se had the highest and lowest mRNA expression of SSCs markers, respectively. In conclusion, the present study indicated that Nano-Se had advantages to sodium Selenite and 0.6 mg/kg of Nano-Se supplemented in males' diet in broiler breeders farms may contributes to optimal fertility via increasing mRNA expression of SSCs markers of roosters' testis and could be used to delay reduction of fertility caused by aging in broiler breeder males.
