Embryology
Zahra Aaly-Gharibeh; Mohammadreza Hosseinchi; Ali Shalizar-Jalali
Volume 15, Issue 2 , February 2024, , Pages 113-117
Abstract
The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, in vitro fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. ...
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The precise pathophysiology of polycystic ovary syndrome (PCOS) is not well-founded. In an attempt to fill this gap, the current study was executed to probe the effect of nanocurcumin (NCC) on ovarian tissue, in vitro fertilization (IVF) and pre-implantation embryo development in a mouse model of PCOS. Fifty adult female mice were randomly categorized into five equal groups including non-treated control and PCOS (receiving 0.20 mg estradiol valerate (EV) intra-peritoneally once a day for 21 days) as well as NCC12.50 + PCOS, NCC25 + PCOS and NCC50 + PCOS (receiving respectively 12.50, 25.00 and 50.00 mg kg-1 NCC daily along with EV injection through oral gavages for 21 days) groups. Subsequently, ovarian histo-architecture and total anti-oxidant capacity, and malonaldehyde and catalase levels as well as in vitro fertilizing potential, early embryonic development and serum testosterone concentration were analyzed. Results showed that NCC in a dose-dependent manner improved ovarian cyto-architectural organization and oxidant/anti-oxidant balance along with IVF rate and pre-implantation embryo development in PCOS mice. These findings revealed that NCC at the doses of 25.00 and 50.00 mg kg-1 could alleviate PCOS-linked reproductive disruptions in female mice.
Ramin Jahangirfard; Gholamreza Najafi; Ali Shalizar-Jalali; Abbas Ahmadi; Elham Zadehhashem
Volume 12, Issue 3 , September 2021, , Pages 333-338
Abstract
Ethephon (C2H6ClO3P; ETP), an organophosphorus pesticide regulating plant growth, is widely used for early ripening of fruits and vegetables in agriculture. The aim of this study was to investigate the effects of ETP on histomorphometrical and biochemical parameters in mouse testicular tissue. In this ...
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Ethephon (C2H6ClO3P; ETP), an organophosphorus pesticide regulating plant growth, is widely used for early ripening of fruits and vegetables in agriculture. The aim of this study was to investigate the effects of ETP on histomorphometrical and biochemical parameters in mouse testicular tissue. In this study, 90 adult male mice were randomly divided into six equal groups (n = 15). The ETP was administered orally at different doses (120, 240 and 480 mg kg-1) daily for 35 days. Untreated control, sham (received only normal saline) and neostigmine bromide-treated (positive control; 0.10 mg kg-1 orally; once per week) groups were also considered. Following 35 days, animals were euthanized and testicle and serum samples were taken. Accordingly, blood and serum acetylcholinesterase (AChE), catalase, total antioxidant capacity (TAC) and malondialdehyde (MDA) levels as well as histomorphometrical changes of testicles were investigated. The ETP-administered animals represented a significant reduction in AChE, TAC and catalase levels and remarkable increment in MDA content. A marked reduction was also seen in the germinal epithelium height, connective tissue thickness, seminiferous tubules diameter and Leydig cell number as well as spermiogenesis and Sertoli cell indices in ETP-treated mice compared to control ones. Similar findings were found in neostigmine bromide-treated animals. In conclusion, the ETP significantly affects the serum and blood anti-oxidant statuses and results in severe histological damages both at germ and somatic cell levels, suggesting its hematotoxic and reprotoxic characteristic.
Abbas Sadeghi; Farah Farokhi; Ali Shalizar Jalali; Gholamreza Najafi
Volume 11, Issue 3 , September 2020, , Pages 257-263
Abstract
Polyvinyl chloride (PVC) has toxic effects through the induction of oxidative stress in the body and testicles. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging toxic free radicals. The present study aimed to probe the protective effect of Vit E against PVC-induced ...
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Polyvinyl chloride (PVC) has toxic effects through the induction of oxidative stress in the body and testicles. Vitamin E (Vit E) is a dietary compound that functions as an antioxidant scavenging toxic free radicals. The present study aimed to probe the protective effect of Vit E against PVC-induced reprotoxicity in male rats. In this experimental study, 24 male rats were randomly divided into four groups (n=6) including control, Vit E (150 mg kg-1 per day; orally), PVC (1000 mg kg-1 per day; orally) and PVC + Vit E. After 40 days, rats were euthanized and epididymal sperms characteristics, embryo development and malondialdehyde (MDA) and testosterone levels were examined. The PVC decreased sperm count, motility and viability as well as testosterone level and increased sperms with damaged chromatin in comparison with controls. Also, the percentages of fertilization, two-cell embryos and blastocysts as well as MDA levels were decreased in PVC-treated rats. However, Vit E improved PVC-induced alterations in aforesaid parameters. The results indicated that PVC can reduce fertility potential in male rats probably through androgen and sperm quality and quantity reductions, while Vit E can exert protective effects in PVC-related reproductive toxicities.
Pharmacology
Alireza Nourian; Ali Soleimanzadeh; Ali Shalizar Jalali; Gholamreza Najafi
Volume 8, Issue 4 , December 2017, , Pages 341-345
Abstract
Bisphenol-S (BPS) is a new bisphenol-A substitute widely used in many plastic products. Bisphenol-A as a main member of bisphenol family has been known as an endocrine system disrupter chemical compound. Like other members of bisphenol family, there is public health concern about the toxic effects of ...
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Bisphenol-S (BPS) is a new bisphenol-A substitute widely used in many plastic products. Bisphenol-A as a main member of bisphenol family has been known as an endocrine system disrupter chemical compound. Like other members of bisphenol family, there is public health concern about the toxic effects of BPS on reproductive system, thus, we examined BPS effects on in vitro fertilization (IVF) potential and oxidative stress status in a murine model. Adult female mice (n = 70) were randomly divided into control and BPS-treated groups. Bisphenol-S was administered at doses of 0, 1, 5, 10, 50 and 100 µg kg-1 body weight per day intraperitoneally for 21 consecutive days. Twenty-Four hr after the last treatment, five mice in each group were super-ovulated and the oocytes were harvested for IVF. All ovaries were collected and used for biochemical factors analyses. Bisphenol-S exposure at doses more than 10 µg kg-1 induced developmental arrest of pre-implantation embryos. Further, lipid peroxidation measurement in ovaries indicated that all doses of BPS cause oxidative stress in female mice. In conclusion, BPS administration even in low doses can result in female reproductive toxicities and oxidative stress in mice.
Anatomy
Naeimeh Simaei; Farhad Soltanalinejad; Gholamreza Najafi; Ali Shalizar Jalali
Volume 8, Issue 3 , September 2017, , Pages 237-241
Abstract
The middle ear ossicles are important due to transmission of sound to the inner ear leading to sound understanding. The aim of present study was to determine the anatomical and morphometrical aspects of middle ear ossicles in Makouei sheep fetuses. For this experimental study, eight sheep fetuses at ...
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The middle ear ossicles are important due to transmission of sound to the inner ear leading to sound understanding. The aim of present study was to determine the anatomical and morphometrical aspects of middle ear ossicles in Makouei sheep fetuses. For this experimental study, eight sheep fetuses at the age of 2 to 3 months were provided from public slaughterhouse; their middle ear ossicles were removed from tympanic cavity subsequently and assessed anatomically using stereomicroscope. For statistical analysis, one-way ANOVA and Tukey’s post-hoc test were used. The results showed that rostral process of malleus doesn’t exist, but an osseous lamina extending to the tympanic bulla and tympanic ring is located in this place. Moreover, lenticular bone and muscular process of stapes weren’t found. These findings were similar to the other animal’s ossicles anatomical features, but there were also some differences that can be useful for study of these ossicles developmental evolution.
Histology
Ameneh Hasanzadeh Khosh; Shapour Hasanzadeh; Ali Shalizar Jalali
Volume 8, Issue 2 , June 2017, , Pages 97-104
Abstract
Nicotine (NIC) adversely influences male reproductive system. Achillea millefolium (Achm) as a medicinal plant is highly regarded for its antioxidant and anti-inflammatory properties. The present study was conducted to assess whether Achminflorescences alcoholic extract could serve as a protective agent ...
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Nicotine (NIC) adversely influences male reproductive system. Achillea millefolium (Achm) as a medicinal plant is highly regarded for its antioxidant and anti-inflammatory properties. The present study was conducted to assess whether Achminflorescences alcoholic extract could serve as a protective agent against reproductive toxicity in NIC-exposed male rats. Adult male rats were randomly divided into six groups. Two groups received NIC at doses of 0.20 and 0.40 mg kg-1 per day in 0.50 mL sterile distilled water for 48 days intraperitoneally, respectively. The further two groups received NIC at doses of 0.20 and 0.40 mg kg-1 per day in 0.50 mL sterile distilled water for intraperitoneally along with Achm extract at a dose of 1.20 g kg-1 per day in 1 mL sterile distilled water orally for 48 days, respectively. A vehicle treated control group and an Achm-only treated group were also included. The NIC-exposed groups showed significant reductions in epididymal sperm count, motility, viability and serum levels of FSH, LH and testosterone as well as testicular antioxidant capacity. Moreover, the incidence of apoptosis and abnormality in spermatozoa along with testicular malondialdehyde and total nitrite levels were significantly higher in NIC-treated rats. The above-mentioned parameters were restored to near normal levels by Achm co-administration. These findings indicated thatAchmmay partially be protective against NIC-induced testicular toxicity.
