Mohammad Ali Faraji; Hossein Hassanpour; Shahab Bahadoran; Waranyoo Kaewduangta; Hamed Zarei; Morteza Hosseininejad; Tahere Karimi Shayan
Volume 11, Issue 4 , December 2020, , Pages 371-376
Abstract
The effect of Shirazi thyme as a medicinal plant on oxidant status (lipid peroxidation, protein oxidation, total antioxidant capacity and catalase activity) and absorptive surface area were measured in three segments of small intestine in cold-induced pulmonary hypertensive chickens. Birds were reared ...
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The effect of Shirazi thyme as a medicinal plant on oxidant status (lipid peroxidation, protein oxidation, total antioxidant capacity and catalase activity) and absorptive surface area were measured in three segments of small intestine in cold-induced pulmonary hypertensive chickens. Birds were reared at 4 groups (thyme 0, 0.25, 0.5 and 1 % of diet) for 42 days. To induce pulmonary hypertension, the temperature was gradually decreased. The body weight was increased in thyme-0.25% birds in compared to control ones while it was decreased in thyme-1% birds. The feed consumption was only increased in thyme-1% birds. The feed conversion ratio was lower in thyme-0.25% birds and higher in thyme-1% birds than control ones. The duodenal and jejunal villus surface area was lower in thyme-1% birds than control ones while it was greater in the thyme-0.5% birds. The ileal villus surface area and duodenal laminae properia thickness was also greater in thyme-0.5% birds. Lipid peroxidation was only decreased in the duodenum and ileum of thyme-0.5% birds compared to control ones, whereas it was increased in the duodenum and jejunum of thyme-1% birds. Catalase activity was only elevated in the duodenum and jejunum of thyme-1% fed chickens. Total antioxidant capacity was increased in the duodenum, jejunum and ileum of thyme-0.5% birds. It is concluded that Shirazi thyme has beneficial effects on growth performance, intestinal absorptive surface area / secretory system and pulmonary hypertension response at low doses (0.25 and 0.5% fed) whereas high dose of this plant may be toxic (1% fed).
Theriogenology
Hossein Hassanpour; Valiallah Khalaji-Pirbalouty; Manoochehr Adibi; Hassan Nazari
Volume 8, Issue 3 , September 2017, , Pages 251-257
Abstract
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors of transcription factors composed of three family members: PPARα, PPARβ/δ and PPARγ. This study was aimed to evaluate the role of PPARs in the estradiol production via follicle stimulating hormone (FSH) in ...
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Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors of transcription factors composed of three family members: PPARα, PPARβ/δ and PPARγ. This study was aimed to evaluate the role of PPARs in the estradiol production via follicle stimulating hormone (FSH) in the ovine Sertoli cells. At the first step, transcripts of PPARα, PPARβ /δ and PPARγ were evaluated by quantitative real time PCR (qRT-PCR) in the ovine Sertoli cells in vitro after FSH treatment. PPARγ transcript was increased in FSH-treated cells while PPARα and PPAR β /δ transcripts were unchanged. At the second step, Pioglitazone as PPARγ agonist and 2-chloro-5-nitrobenzanilide (GW9662) as PPARγ antagonist were used in the FSH-treated Sertoli cells and then, the estradiol production and aromatase transcript were evaluated. Aromatase transcript was increased by pioglitazone in the FSH-treated Sertoli cells while GW9662 did not change its transcript. The estradiol production was increased by low concentrations of pioglitazone in FSH-treated Sertoli cells while the production of this hormone was decreased by the high concentration of Pioglitazone. The GW9662 did not change the production of estradiol in FSH-treated Sertoli cells. It is concluded that FSH regulates the estradiol production and aromatase expression in a way independently of PPARβ/δ and PPARα activation, although FSH increases the transcript of PPARγ and in this way, it could affect (mostly increase) aromatase transcript and estradiol production. Probably, this effect of FSH in the estradiol production via PPARγ is only a servo-assist mechanism which if it was inhibited, the estradiol production was not considerably affected.
Theriogenology
Ali Kadivar; Heidar Heidari Khoei; Hossein Hassanpour; Hamid Ghanaei; Arefeh Golestanfar; Hossein Mehraban; Najmeh Davoodian; Roohollah Dehghani Tafti
Volume 7, Issue 1 , March 2016, , Pages 27-34
Abstract
Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype ...
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Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PPARγ and PPARβ/δ was evaluated and compared with high and low motile ram spermatozoa. Semen samples from 6 adult rams were fractionated on a two layer discontinuous Percoll gradient to high and low motile sperm and quantitative parameters of sperm motility were determined by CASA. Total RNA was extracted and the mRNA abundance for each gene was measured by relative quantification technique with Real time PCR. The levels of three isotypes of PPAR transcripts were significantly higher in high motile semen samples using quantitative RT-PCR. Some of sperm motility indices were also significantly correlated with PPARα and PPARγ relative expression. This study revealed the novel association of PPAR gene isotypes with sperm motility. Data from our study suggested PPARs are one of the possible factors that can be studied in male infertility.