Stem Cells
Ezzatollah Fathi; Raheleh Farahzadi; Reza Rahbarghazi; Hossein Samadi Kafil; Rahman Yolmeh
Volume 8, Issue 2 , June 2017, , Pages 89-96
Abstract
Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in ...
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Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO4 and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO4); group III (ZnSO4 treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO4). In the presence of different concentrations of ZnSO4,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO4. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO4 could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO4 may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression. Zinc as an essential trace element was reported to be involved in regulation of the growth and aging of cells. In this study, rat adipose-derived mesenchymal stem cells were exposed to extremely low frequency electromagnetic field (ELF-EMF) of 50 Hz and 20 mT to evaluate whether exposure to ELF-EMF in the presence of zinc sulfate (ZnSO4) affects the telomerase reverse transcriptase (TERT) gene expression and aging in mesenchymal stem cells (MSCs). The cell plates were divided into four groups including group I (control without ZnSO4 and ELF-EMF exposure); group II (ELF-EMF-exposure without ZnSO4); group III (ZnSO4 treatment without ELF-EMF exposure) and group ІV (ELF-EMF exposure with ZnSO4). In the presence of different concentrations of ZnSO4,cells viability, TERT gene expression and percentage of senescent cells were evaluated using colorimetric assay, real-time PCR and senescence-associated β-galactosidase activity assay, respectively. In this experiment, cells were exposed to ELF-EMF for 30 min per day for 21 days in the presence and absence of ZnSO4. The results revealed that ELF-EMF leads to a decrease in the expression of TERT gene and increase in the percentage of senescent cells. However, the ZnSO4 could significantly increase the TERT gene expression and decrease the aging of ELF-EMF-exposed MSCs. It seems that ZnSO4 may be a beneficial agent to delay aging of ELF-EMF-exposed MSCs due to the induction of TERT gene expression.
Mohammad Tolouei Kaleibar; Javad Ashrafi Helan; Ezzatollah Fathi
Volume 5, Issue 3 , July 2014, , Pages 237-241
Abstract
An 8-day-old newborn female twin Holstein calves with a history of weakness, anorexia, emaciation and convulsion were presented to Tabriz University Veterinary Teaching Hospital. On admission, the calves were febrile and recumbent. Physical examination revealed many ticks from the external body surface ...
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An 8-day-old newborn female twin Holstein calves with a history of weakness, anorexia, emaciation and convulsion were presented to Tabriz University Veterinary Teaching Hospital. On admission, the calves were febrile and recumbent. Physical examination revealed many ticks from the external body surface of the animals, right and left prescapular lymphadenopathy, severe opisthotonos, nystagmus, pedaling, blindness, hyperpnea and hypersthenia. Buccal and vaginal mucous were pale and no other physical abnormalities were diagnosed. Fecal flotation, complete blood count, bone marrow aspiration, cerebrospinal fluid (CSF) analysis, necropsy and histopathological examination were performed. Fecal flotation showed no helminth eggs or coccidial oocysts. On blood smears obtained from the earlap, >70 percent of erythrocytes were infected with piroplasms organisms and schizonts were obvious in smears of lymphocytes lymph node. Blood count revealed a lymphopenia, poikilocytosis, anisocytosis and non-regenerative anemia (packed cell volume; mean, 16%). Histopathological examination revealed Arthus reaction through the walls of cerebral blood vessels, which resulted in local necrosis of the brain. Analysis of CSF showed no abnormality in appearance or biochemical and cell counts. Although the calves were treated with a single intramuscular injection of buparvaquone and oxytetracycline once daily they did not respond to the treatment and died. In conclusion, the present cases showed a rare cerebral form of theileriosis by vertical transmission that confirmed by the presence of piroplasms on blood films and multinuclear schizonts on lymph node aspiration smears, gross and histopathological examinations and unsuccessful treatment in a newborn twin Holstein calves.