Theriogenology
Sayed Mortaza Alavi Shoushtari; Siamak Asri Rezaie; Amir Khaki; Abulfazl Belbasi; Hamid Tahmasebian
Volume 6, Issue 3 , September 2015, , Pages 211-215
Abstract
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 ...
Read More
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 were pro-estrous, estrous, met-estrous and diestrous, respectively. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean (± SEM) total serum (77.10 ± 1.50 µg dL-1) and uterine fluid (296.40 ± 9.40 μg dL-1) Cu in cyclic cows was higher than the values of 54.00 ± 1.10 μg dL-1 and 133.40 ± 5.70 μg dL-1 in pre-pubertal calves, respectively. Blood serum (114.60 ± 3.20 μg dL-1) and the uterine fluid (349.90 ± 8.90 μg dL-1) Zn content in cyclic cows were also higher than those (98.80 ± 1.50 μg dL-1 and 246.6 ± 4.50 μg dL-1 respectively) in pre-pubertal calves. Serum Cu in pro-estrus and estrus were lower than those in other stages and also lower than those in the uterine fluid. The lowest serum Zn content was recorded in pro- and met-estrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle the uterine fluid Zn content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Cu and Zn were actively secreted in uterine lumen and were not dependent on blood serum. The values also increased after puberty.
Sayed Mortaza Alavi Shoushtari; Siamak Asri Rezaie; Amir Khaki; Abulfazle Belbasi; Hamid Tahmasebian
Volume 5, Issue 4 , December 2014, , Pages 301-305
Abstract
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, ...
Read More
To investigate uterine fluid and serum calcium (Ca) and Magnesium (Mg) variations during the estrus cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia. The phase of the estrous cycle was determined by examining ovarian structures. 18 animals were pro-estrous, 15 estrous, 16 met-estrous and 22 diestrous. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean ± SEM total serum and uterine fluid Ca in cyclic buffaloes were 8.68 ± 0.28 mg dL-1 and 8.10 ± 0.2 mg dL-1 vs. 6.76 ± 0.65 mg dL-1 and 7.90 ± 0.15 mg dL-1 in pre-pubertal calves, respectively. Blood serum Mg was not different in cyclic and pre-pubertal animals but the uterine fluid Mg in cyclic cows was higher than those in pre-pubertal calves. Serum Ca in pro-estrus and estrus were higher than those in other stages and also higher than those in the uterine fluid. The lowest Mg content of serum was recorded in diestrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle except for estrus the uterine fluid Mg content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Ca was passively secreted in uterine lumen and mostly dependent on blood serum Ca concentrations but Mg was secreted independently. The values (except for serum total Mg) also increased after puberty.
Sayed Mortaza Alavi-Shoushtari; Roya Abedizadeh; Amir Khaki; Aram Mokarizadeh; Kamran Dorostkar
Volume 5, Issue 2 , June 2014, , Pages 115-119
Abstract
To investigate the IgG content and its variations in uterine fluid (UF) during the estrous cycle of the cow and to compare them with those of the blood serum (S), six pairs of serum and UF samples for each phase of the cycle selected out of 240 bovine genital tracts and blood samples were collected from ...
Read More
To investigate the IgG content and its variations in uterine fluid (UF) during the estrous cycle of the cow and to compare them with those of the blood serum (S), six pairs of serum and UF samples for each phase of the cycle selected out of 240 bovine genital tracts and blood samples were collected from Urmia abattoir. The UF samples were collected by gentle scraping of the endometrium using a curette after uterine incision and their IgG content and those of the serum were measured by single radial immuno-diffusion (SRID) assay. Serum IgG values (Mean ± SEM) were generally higher than the UF values throughout the cycle except for di-estrus (S: 38.50 ± 0.90, UF: 51.60 ± 2.10 mg mL-1), in which the highest values were observed in UF samples. In met-estrus the difference was not significant (S: 34.80 ± 1.80mg mL-1, UF: 30.80 ± 5.20 mg mL-1), however, in estrus the mean UF IgG value (12.50 ± 1.10 mg mL-1) was lower than that of the serum (31.30 ± 1.20 mg mL-1). In pro-estrus, the lowest values (S: 27.80 ± 1.30 mg mL-1, UF: 9.10 ± 1.50 mg mL-1) were obtained. The results showed a lower IgG values in the bovine UF than those of the serum in the follicular phase of the cycle, while in di-estrus the UF IgG content was the highest, suggesting some IgG production in the uterus at this phase.