Pathology
Ayhan Atasever; Ali Sefa Mendil; Mehmet Ozkan Timurkan
Volume 14, Issue 10 , October 2023, , Pages 541-548
Abstract
This study aimed to investigate the potential presence of bovine herpes virus type 1 (BHV-1) and bovine viral diarrhea virus (BVDV) in cattle uteri that did not display any clinical and macroscopic signs of infection. Virus detection involved polymerase chain reaction (PCR) test, double immunohistochemistry ...
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This study aimed to investigate the potential presence of bovine herpes virus type 1 (BHV-1) and bovine viral diarrhea virus (BVDV) in cattle uteri that did not display any clinical and macroscopic signs of infection. Virus detection involved polymerase chain reaction (PCR) test, double immunohistochemistry (IHC), and double immunofluorescence (IF). One hundred cornu uterus samples were collected from cattle aged 1 year and older. The BVDV was detected by PCR or by double IHC/IF in the collected samples from slaughterhouses in Kayseri city (Central Anatolia, Türkiye) from 2021 - 2022. By contrast, BHV-1 was detected by PCR and double IHC/IF at a rate of 16.00% and 21.00%, respectively. In the IHC and IF detection, BHV-1 was detected in endometrial epithelial cells and in some mononuclear cells in the lamina propria, periglandular areas and myometrium. Although no macroscopic lesion was found in the BHV-1-positive samples (n = 21), histopathological detection showed that two had acute endometritis, eight had subacute endometritis, eight had chronic endometritis and the three others showed no signs of endometritis. This prevalence study demonstrated for the first time that even while BVDV could not be detected in the samples, BHV-1 posed a critical potential reproductive risk in pregnant animals, as it can specifically cause abortions when it resides in cattle uteri that do not show clinical or macroscopic and even microscopic signs of infection. Additionally, this study was the first to combine PCR and double IHC/IF for BHV-1 and BVDV detection in cattle uteri.
Asghar Mogheiseh; Abdollah Derakhshandeh; Sara Heidarifar; Esmaeil Bandariyan
Volume 11, Issue 1 , March 2020, , Pages 89-92
Abstract
The microbial population of the uterus fluctuates during the estrous cycle. Microflora of uterus may affect the establishment and maintenance of pregnancy in bitches. The endoscopic samples obtained from the vagina and uterus of 20 female adult mixed‑breed dogs. The uterine lavage samples were prepared ...
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The microbial population of the uterus fluctuates during the estrous cycle. Microflora of uterus may affect the establishment and maintenance of pregnancy in bitches. The endoscopic samples obtained from the vagina and uterus of 20 female adult mixed‑breed dogs. The uterine lavage samples were prepared for cytology, bacterial (aerobic and anaerobic) and fungal cultures. Uterine tissue samples were evaluated for the presence of E. coli by the polymerase chain reaction. The pure growth of bacteria was observed in seven plates out of the nineteen cultured samples (36.84%) and five Gram-negative and two Gram-positive bacteria were detected. The highest number of isolated bacteria was related to the samples of the diestrus and anestrus stages of the estrous cycle, while the lowest number of bacteria was observed in the samples of the estrous stage. Moreover, Citrobacter spp. was the most frequent group of isolated bacteria. The neutrophils were detected in the cytology of uterine samples. The fungi growth was observed in three uterine samples. Cladosporium and Penicillium isolated from the samples were related to the estrus stage, and yeast was grown in diestrus samples. 16srRNA gene existed in all of the estrous uterine samples in which the bacterial culture was negative. However, the presence of this gene was proven in two samples (33.30%) of negative bacterial culture samples from the diestrus and anestrus stages. In conclusion, the normal bitches’ uteri were infected with various bacteria in estrus, diestrus and anestrus stages of the estrous cycle, and it could coincide with the fungi infection.
