Sarang Soroori; Soraya Saleh Gargari; Nima Sayyah; Mohammad Reza Esmaili Nejad
Volume 11, Issue 2 , June 2020, , Pages 165-170
Abstract
This study was performed to achieve abortion in a short time and with minimum side effects in a bitch, as an alternative method for termination of unwanted pregnancy. The experimentation was performed on 10 privately owned crossbred pregnant bitches, in their late second trimester of pregnancy, having ...
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This study was performed to achieve abortion in a short time and with minimum side effects in a bitch, as an alternative method for termination of unwanted pregnancy. The experimentation was performed on 10 privately owned crossbred pregnant bitches, in their late second trimester of pregnancy, having a variable number of fetuses (3 to 9). Fetal death was induced by transabdominal intracardiac injection of potassium chloride (KCl) into the fetal heart under ultrasonographic guidance. Pregnancy was terminated within 36 to 72 hr (51.60 ±16.04 hr) and none of the patients experienced any side effects or clinical complications. Data presented in this report provided evidence for the possible use of this technique to selectively reduce the number of canine fetuses without terminating the whole pregnancy. Ultrasound-guided induced fetal death is a safe procedure and a viable method for the induction of abortion in a short time and with no apparent side effects.
Asghar Mogheiseh; Abdollah Derakhshandeh; Sara Heidarifar; Esmaeil Bandariyan
Volume 11, Issue 1 , March 2020, , Pages 89-92
Abstract
The microbial population of the uterus fluctuates during the estrous cycle. Microflora of uterus may affect the establishment and maintenance of pregnancy in bitches. The endoscopic samples obtained from the vagina and uterus of 20 female adult mixed‑breed dogs. The uterine lavage samples were prepared ...
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The microbial population of the uterus fluctuates during the estrous cycle. Microflora of uterus may affect the establishment and maintenance of pregnancy in bitches. The endoscopic samples obtained from the vagina and uterus of 20 female adult mixed‑breed dogs. The uterine lavage samples were prepared for cytology, bacterial (aerobic and anaerobic) and fungal cultures. Uterine tissue samples were evaluated for the presence of E. coli by the polymerase chain reaction. The pure growth of bacteria was observed in seven plates out of the nineteen cultured samples (36.84%) and five Gram-negative and two Gram-positive bacteria were detected. The highest number of isolated bacteria was related to the samples of the diestrus and anestrus stages of the estrous cycle, while the lowest number of bacteria was observed in the samples of the estrous stage. Moreover, Citrobacter spp. was the most frequent group of isolated bacteria. The neutrophils were detected in the cytology of uterine samples. The fungi growth was observed in three uterine samples. Cladosporium and Penicillium isolated from the samples were related to the estrus stage, and yeast was grown in diestrus samples. 16srRNA gene existed in all of the estrous uterine samples in which the bacterial culture was negative. However, the presence of this gene was proven in two samples (33.30%) of negative bacterial culture samples from the diestrus and anestrus stages. In conclusion, the normal bitches’ uteri were infected with various bacteria in estrus, diestrus and anestrus stages of the estrous cycle, and it could coincide with the fungi infection.
