Theriogenology
Hossein Hassanpour; Valiallah Khalaji-Pirbalouty; Manoochehr Adibi; Hassan Nazari
Volume 8, Issue 3 , September 2017, , Pages 251-257
Abstract
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors of transcription factors composed of three family members: PPARα, PPARβ/δ and PPARγ. This study was aimed to evaluate the role of PPARs in the estradiol production via follicle stimulating hormone (FSH) in ...
Read More
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors of transcription factors composed of three family members: PPARα, PPARβ/δ and PPARγ. This study was aimed to evaluate the role of PPARs in the estradiol production via follicle stimulating hormone (FSH) in the ovine Sertoli cells. At the first step, transcripts of PPARα, PPARβ /δ and PPARγ were evaluated by quantitative real time PCR (qRT-PCR) in the ovine Sertoli cells in vitro after FSH treatment. PPARγ transcript was increased in FSH-treated cells while PPARα and PPAR β /δ transcripts were unchanged. At the second step, Pioglitazone as PPARγ agonist and 2-chloro-5-nitrobenzanilide (GW9662) as PPARγ antagonist were used in the FSH-treated Sertoli cells and then, the estradiol production and aromatase transcript were evaluated. Aromatase transcript was increased by pioglitazone in the FSH-treated Sertoli cells while GW9662 did not change its transcript. The estradiol production was increased by low concentrations of pioglitazone in FSH-treated Sertoli cells while the production of this hormone was decreased by the high concentration of Pioglitazone. The GW9662 did not change the production of estradiol in FSH-treated Sertoli cells. It is concluded that FSH regulates the estradiol production and aromatase expression in a way independently of PPARβ/δ and PPARα activation, although FSH increases the transcript of PPARγ and in this way, it could affect (mostly increase) aromatase transcript and estradiol production. Probably, this effect of FSH in the estradiol production via PPARγ is only a servo-assist mechanism which if it was inhibited, the estradiol production was not considerably affected.
Arumugam Kumaresan; Subhash Chand; Sankaran Suresh; Tushar Kumar Mohanty; Shiv Prasad; Siddhartha Shankar Layek; Kumaresh Behera
Volume 4, Issue 2 , June 2013, , Pages 85-89
Abstract
Four crossbred cows with mummified fetus were utilized for the study. The cows were subjected to gynecological examination and based on the findings the cases were diagnosed as mummified fetus. The cows were treated with 2 mg estradiol valerate and 500 µg cloprostenol and were examined every 12 ...
Read More
Four crossbred cows with mummified fetus were utilized for the study. The cows were subjected to gynecological examination and based on the findings the cases were diagnosed as mummified fetus. The cows were treated with 2 mg estradiol valerate and 500 µg cloprostenol and were examined every 12 hr after 24 hr of the treatment for cervical dilatation and other signs related to fetal expulsion. The time duration between treatment and starting of cervical dilatation ranged from 48 to 58h (53.00 ± 2.08 hr). Complete dilatation of cervix was observed after 70.00 ± 2.94 hr post treatment (Range = 64-76 hr). The mean fetal crown-rump length (CRL) was 31.5 cm, which ranged from 27.5 to 38 cm. The number of cotyledons in pregnant horn also showed wide variation (Range 24-38 numbers) with mean ± SE of 30.3 ± 3.07 numbers. In the placenta of three animals irregular shaped large adventitious cotyledons were observed in the inter-cotyledonary areas. Out of the four animals treated, three animals were conceived within three estrous cycles and one animal had cystic ovary in the next cycle and was not conceived even after four cycles. It was concluded that the estradiol and prostaglandin F2α (PGF2α) combination therapy was effective for expulsion of mummified fetus in crossbred cows without affecting much on future fertility.