Muhammad Khurram Shahzad; Imtiaz Rabbani; Syed Murtaza Hassan Andrabi; Hafsa Zaneb; Muhammad Shahbaz Yousaf; Khalid Abdul Majeed; Sajid Khan Tahir; Sohrab Ahmad; Habib Rehman
Volume 12, Issue 3 , September 2021, , Pages 283-289
Abstract
Kisspeptin has an important role in the stimulation of hypothalamic-pituitary-gonadal axis in term of pubertal development, release of reproductive and metabolic hormones and ultimately affecting the fertility. The aim of the present study was to evaluate the serum kisspeptin level and its correlation ...
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Kisspeptin has an important role in the stimulation of hypothalamic-pituitary-gonadal axis in term of pubertal development, release of reproductive and metabolic hormones and ultimately affecting the fertility. The aim of the present study was to evaluate the serum kisspeptin level and its correlation with semen quality and selected hormones in buffalo bulls during the summer and spring seasons. Semen and blood samples from eight Nili-Ravi buffalo bulls (age: 9.21 ± 1.02 years) were collected. Semen was analysed using computer-assisted semen analysis. Serum concentrations of kisspeptin, gonadotropin releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, cortisol, triiodothyronine (T3), thyroxin (T4) and insulin like growth factor (IGF-1) were estimated using enzyme-linked immunosorbent assay kits. Kisspeptin was neither affected by seasons and nor correlated with semen parameters and hormones. Higher levels of GnRH, LH, cortisol, IGF-1, total motility (TM), average path velocity (VAP), straight-line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN) were recorded in summer compared to spring. Correlations of GnRH versusIGF-1 and LH, LH versus IGF-1 and cortisol, FSH versus T4 and testosterone, testosterone versus T3 and T4 and T3 versus T4 were observed. The GnRH and IGF-1 were positively associated with TM, VAP, VSL, VCL and LIN. The LH was correlated with VSL, straightness and LIN. In conclusion, GnRH, LH, and IGF-1 correlations with semen parameters can be used to indicate semen quality. The buffalo bulls are well-adapted and can give quality semen in the summer season.
Zahra Salemi; Annahita Rezaie; Saad Goorani Nejad; Babak Mohammadian
Volume 11, Issue 4 , December 2020, , Pages 409-414
Abstract
This study aimed to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in the water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including ...
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This study aimed to determine cytological, histopathological and cytomorphometrical characteristics of endometrium in healthy and endometritic uterus in the water buffalo. Fifty eight non-pregnant reproductive systems were collected from slaughterhouse. Efficiency of three methods of sampling including cotton swab, smear, and aspiration were compared for cytologic study. Concurrent histopathologic examination revealed endometritis in 38 uteri including 8 (21.00%) with mild endometritis, 7 (18.42%) with moderate endometritis, 6 (15.90%) with severe endometritis and 17 (44.73%) with chronic endometritis. Cytomorphometrical results showed significant relationship between diameter and area of epithelial nuclei with phases of estrus cycle. Neutrophil and lymphocytes densities in swab and aspiration samples were significantly higher in severe endometritis than normal and chronic endometritis samples. Similarly, lymphocytes density in smear and aspiration methods was significant between normal and moderates, and also severe and chronic endometritis. Cytomorphometric analysis of epithelial nuclei characteristics (diameter and area) in buffalo were performed for the first time and it could be valuable to identify estrus cycle in this species. Aspiration had the most efficiency to identify endometritis in comparison with other methods.
