Mehdi Golchin; Somayye Mollayi; Elham Mohammadi; Neda Eskandarzade
Volume 13, Issue 3 , September 2022, , Pages 387-391
Abstract
Brucellosis is considered as one of the important global zoonotic diseases that causes medical as well as economic problems especially in tropical countries. The illness has no specific pathognomonic signs; therefore, the rapid and accurate diagnosis of the disease has a very important role in preventing ...
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Brucellosis is considered as one of the important global zoonotic diseases that causes medical as well as economic problems especially in tropical countries. The illness has no specific pathognomonic signs; therefore, the rapid and accurate diagnosis of the disease has a very important role in preventing the Brucella spillover and treatment. The purpose of this study was to design a new indirect ELISA test for detection of human brucellosis based on using recombinant Brucella abortus outer membrane protein 16.00 kDa (rOMP16) as an antigen. OMP16 gene of B. abortus was initially synthesized and cloned in pET-21d vector and then expressed in Escherichia coli cells. The expression was confirmed by the SDS-PAGE, western blotting and dot blotting. The purified protein was coated in ELISA plates and an indirect ELISA was performed on 70 human serum samples. The results were evaluated with a commercial IgG ELISA kit and Rose Bengal plate agglutination tests as reference tests. Diagnostic performance of designed OMP16 ELISA test in comparison with Rose Bengal plate test revealed 100% of sensitivity, 95.00% of specificity and good Fleiss kappa agreement, whereas, where it was compared to commercial ELISA kit, it revealed very good kappa agreement with 100% of sensitivity and 100% of specificity in cut-off value of 0.13. It was concluded that OMP 16.00 kDa could be acceptable alternative antigen for detecting Brucella IgG antibody with high accuracy.
Food Hygiene
Mohammadreza Taghdiri; Guiti Karim; Shahabeddin Safi; Abbas Rahimi Foroushani; Abbasali Motalebi
Volume 9, Issue 2 , June 2018, , Pages 179-185
Abstract
Bulk tank somatic cell count (BTSCC) is a gold standard test for identification of milk quality, but its results are influenced by several interventional factors. Recently, application of acute phase proteins and especially milk amyloid A (MMA) has been considered as accurate parameters for milk quality ...
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Bulk tank somatic cell count (BTSCC) is a gold standard test for identification of milk quality, but its results are influenced by several interventional factors. Recently, application of acute phase proteins and especially milk amyloid A (MMA) has been considered as accurate parameters for milk quality study. The current research was done to evaluate the accuracy of MMA, BTSCC, fat, protein and lactose for identification of milk quality. Ninety bulk tank milk samples were collected from 30 randomly selected dairy herds and classified into two groups of samples with BTSSC > 200000 cells per mL and those with BTSSC < 200000 cells per mL. Protein, fat, lactose and MAA contents of samples were analyzed. Average amount of the MAA in healthy and mastitic milk samples were 5.15 and 504.35 ng mL-1, respectively. Statistically significant difference was seen for MAA and total protein contents between two groups. Clinical accuracy of MAA-, total protein-, fat- and lactose–based methods was 0.937, 0.757, 0.665 and 0.547, respectively. The MAA method at concentration of 20.78 ng mL-1 had the highest sensitivity (97.30%) and specificity (46.70%). Evaluation of MAA is recommended as a rapid and accurate method for determination of the unfavorable changes in milk quality and early subclinical mastitis diagnosis.
