Iman Yousefian; Ahmad Zare-Shahneh; Hamid Kohram
Volume 13, Issue 1 , March 2022, , Pages 71-78
Abstract
The effects of MitoTEMPO, a mitochondria-targeted antioxidant, and its non-targeted parent, TEMPO, on bovine oocyte maturation competence have not been determined so far. Hence, our study was aimed to investigate the effects of supplementing maturation medium with different concentrations of MitoTEMPO ...
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The effects of MitoTEMPO, a mitochondria-targeted antioxidant, and its non-targeted parent, TEMPO, on bovine oocyte maturation competence have not been determined so far. Hence, our study was aimed to investigate the effects of supplementing maturation medium with different concentrations of MitoTEMPO (0.00, 0.10, 1.00 and 10.00 µM) or TEMPO (0.00, 5.00, 10.00 and 15.00 mM) on in vitro maturation (IVM) and fertilization (IVF) of bovine oocytes. The oocytes after IVM and IVF were evaluated for the signs of nuclear maturation and normal fertilization. The average number of spermatozoa penetrated per oocyte and the level of intracellular reactive oxygen species (ROS) were also evaluated. The results showed that percentages of bovine oocytes reached the metaphase II stage of meiosis were significantly higher in the 1.00 µM MitoTEMPO group compared to the control group (without antioxidant supplementation). The normal fertilization rate also tended to be greater in this group than the control group. In comparison with the control group, the medium supplementation with 1.00 µM MitoTEMPO led to a significant decrease in the intracellular ROS level. The average number of spermatozoa penetrated per oocyte was not significantly different among the antioxidant-treated and the non-treated groups. The TEMPO addition to the maturation medium affected neither the rate of maturation/fertilization nor the level of intracellular ROS in bovine oocytes. Based on these results, we concluded that MitoTEMPO at a concentration of 1.00 µM had beneficial effects on the quality and fertilization potential of bovine oocytes.
Aghakhan Kheiri; Javad Aliakbarlu; Rahele Tahmasebi
Volume 13, Issue 1 , March 2022, , Pages 91-99
Abstract
The objectives of the present study were to investigate the effect of season and fish species on the antioxidant capacity, fatty acids profile, and vitamin E content of fish fillets from Aras River. The antioxidant potential of hydrophilic and lipophilic extracts of fish fillets was evaluated. The fillet ...
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The objectives of the present study were to investigate the effect of season and fish species on the antioxidant capacity, fatty acids profile, and vitamin E content of fish fillets from Aras River. The antioxidant potential of hydrophilic and lipophilic extracts of fish fillets was evaluated. The fillet extracts of zander and bream in summer and common carp in winter had the highest antioxidant activity. Palmitic and oleic acids were the major saturated (SFA) and monounsaturated (MUFA) fatty acids, respectively. The fatty acids C22:6n3 and C20:5n3 were the most abundant polyunsaturated (PUFA) fatty acids in all the fishes. In summer, the highest levels of SFA (44.09), total PUFA (25.97), n3 PUFA (20.71) and n3/n6 ratio (4) were found in zander. In winter, the highest amounts of total PUFA and n3 PUFA were determined in silver carp, followed by zander. The highest n3/n6 ratio was also found in silver carp. The ratio of PUFA/SFA was higher in winter than in summer for all the fishes. Vitamin E content of fishes was largely varied. In conclusion, seasonal variation changed the antioxidant potential and fatty acid composition of fish fillets. Aras River fishes, especially zander, possess excellent antioxidant activity and high nutritional quality.
Masoumeh Moradi-Ozarlou; Sara Javanmardi; Hossein Tayefi Nasrabadi
Volume 11, Issue 1 , March 2020, , Pages 27-33
Abstract
The present study was aimed to determine the protective effects of Plantago major L (PM) leaf extracts on the testicular torsion/detorsion (T/D)-induced ischemia/reperfusion (I/R) injury in rats. Twenty-four mature male Sprague-Dawley rats, weighing 200-220 g, were selected. They were randomly divided ...
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The present study was aimed to determine the protective effects of Plantago major L (PM) leaf extracts on the testicular torsion/detorsion (T/D)-induced ischemia/reperfusion (I/R) injury in rats. Twenty-four mature male Sprague-Dawley rats, weighing 200-220 g, were selected. They were randomly divided into four groups of six animals each: Sham (sham-operated rats; all the surgical steps were performed but T/D was not induced), TDC (control group; T/D was induced and the right testicular torsion of 720° lasting two hours was followed by detorsion), TDP50 (T/D-operated rats received 50.00 mg kg-1 of PM extract daily for seven days intraperitoneally after detorsion) and TDP100 (T/D-operated rats received 100 mg kg-1 of PM extract daily for seven days intraperitoneally after detorsion). After seven days of treatment, the right testicles were collected. Histopathological and biochemical analyses including levels of malondialdehyde (MDA) and catalase (CAT) and peroxidase activities were determined in testicular tissues of the rats. Tissue sections were taken from testis, Hematoxylin-Eosin staining was done, and the slides were examined by a light microscope. The level of MDA was significantly increased in the testes of the TDC group. The CAT activity levels were decreased significantly after I/R. The post-torsion treatment with PM, particularly at 100 mg kg-1, prevented the increase in lipid peroxidation and reduced the CAT activity levels. The PM also prevented I/R-induced cellular damage and histological changes in the testicular tissues. According to the results of the current study, PM leaf extracts had significant positive effects on the testicular T/D-induced I/R injury. The possible mechanism of reduction in biochemical and histological injuries by PM extracts could be due to antioxidant property.
Ebrahim Ahmadi; Narges Tahmasebian-Ghahfarokhi; Maryam Nafar-Sefiddashti; Marzieh Sadeghi-Sefiddashti; Hosein Hasanpour
Volume 11, Issue 1 , March 2020, , Pages 43-51
Abstract
Most aspects of reproductive function including spermatogenesis, oocyte growth and maturation, early embryonic development, fetal and placental growth, and lactation can be affected by thermal stress. Furthermore, it has been shown that oxidative stress involves in the pathology of thermal stress. Therefore, ...
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Most aspects of reproductive function including spermatogenesis, oocyte growth and maturation, early embryonic development, fetal and placental growth, and lactation can be affected by thermal stress. Furthermore, it has been shown that oxidative stress involves in the pathology of thermal stress. Therefore, the aim of this study was to investigate the impacts of thermal stress on the ovine mature epididymal spermatozoa extracted from testes of slaughtered rams in the presence or absence of an antioxidant. Epididymal spermatozoa were incubated at scrotal (32.00 ˚C), normal body (39.00 ˚C), and hyperthermic temperatures (41.00 ˚C) for 4 hr in the presence or absence of 1 mmol L-1 β-mercaptoethanol. The results demonstrated the high sensitivity of ram epididymal spermatozoa to the hyperthermic temperature at in vitro conditions. In comparison with scrotal temperature, quality parameters of spermatozoa were negatively affected by increase in temperature, as such in the spermatozoa incubated at hyperthermic temperature significant decrease was observed in the viability, DNA integrity and in the majority of motility parameters. Moreover, concentration of lipid peroxidation by-products, thiobarbituric acid reactive substances, were significantly increased. The findings showed that using antioxidant during incubation period had significant protective effect on the viability and motility of incubated spermatozoa not only at the hyperthermic temperature, but also at the scrotal and normal body temperatures. In conclusion the ovine epididymal spermatozoa were sensitive to in vitro thermal stress and it seems that this sensitivity was partly related to the oxidative stress.
Nahid Atyabi; Seyedeh Parastoo Yasini; Seyedeh Missagh Jalali; Hamid Shaygan
Volume 3, Issue 2 , June 2012, , Pages 97-101
Abstract
Nitrite intoxication occurs frequently in ruminants and equines. The most common treatment of this disorder is administration of 1% methylene blue, although the use of some antioxidant agents e.g. vitamins and complementary treatment may also be useful. The aim of this study was to evaluate the in vitro ...
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Nitrite intoxication occurs frequently in ruminants and equines. The most common treatment of this disorder is administration of 1% methylene blue, although the use of some antioxidant agents e.g. vitamins and complementary treatment may also be useful. The aim of this study was to evaluate the in vitro antioxidative effects of some vitamins on methemoglobinemia induced by sodium nitrite. For this purpose the blood sample of a healthy dairy cattle was pre-incubated with three different concentrations (5, 10, 20 mmol L-1) of each vitamin (E, C, B1, A and a combination of vitamin E and vitamin C) as antioxidant agent at 4 °C for 24 hours. A control group with normal saline instead of vitamin was applied. Then, all samples were treated with sodium nitrite (10 mmol L-1) as an oxidant agent for 10 minutes and the level of methemoglobin formation was measured spectrophoto-metrically. The results revealed that the level of methemoglobin decreased significantly (P < 0.05), when vitamin E (10 and 20 mmol L-1) and vitamin C (5 mmol L-1) was applied to the tests, separately. Vitamin C at the concentration of 20 mmol L-1, was not effective, but it even increased methemoglobin formation significantly. Combination of vitamin E and C was significantly effective at concentration 5 mmol L-1, but not at concentration 10 and 20 mmol L-1. Vitamin A and vitamin B1 were not effective in any concentration. It was concluded that vitamins especially vitamin C and E can reduce oxidative effects which induced methemoglobin formation in vitro and could be used as an alternative medication.
Reza Asadpour; Razi Jafari; Hossein Tayefi - Nasrabad
Volume 2, Issue 1 , March 2011, , Pages 37-44
Abstract
The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/mL with citrate –egg yolk (CEY) or Tris – ...
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The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to 5 aliquots including control and 4 experimental groups. Each aliquot was further diluted with an equal volume of CEY or a Tris (hydroxymethyl) aminomethane (TRIS) - based extender without (control) or containing vitamin C 1mM or 2mM and vitamin E 0.1mM or 0.2mM, and routine semen evaluations like sperm motility, viability and measurement of lipid peroxidation (LPO) were conducted. Significant reductions of LPO were achieved by addition of 1mM vitamin C and 0.1 mM vitamin E to CEY extender (P < 0.05). The highest sperm viability was achieved by addition of 0.1mM vitamin E to CEY extender. Supplementing CEY extender with 2mM vitamin C and 0.1mM vitamin E improved the sperm motility compared with the control group. On the basis of the present results it is concluded that vitamin C and E are very efficient antioxidants in CEY extender.