Tahmineh Gorgani-Firouzjaee; Narges Kalantari; Salman Ghaffari
Volume 13, Issue 3 , September 2022, , Pages 371-378
Abstract
Toxoplasmosis, a foodborn disease, in human occurs commenly after the ingestion of tissue cysts via the raw and/or undercooked meat of different infected intermediate hosts such as sheep and cattle.The current study aimed to detect the genetic structure of Toxoplasma gondii isolated from various organs ...
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Toxoplasmosis, a foodborn disease, in human occurs commenly after the ingestion of tissue cysts via the raw and/or undercooked meat of different infected intermediate hosts such as sheep and cattle.The current study aimed to detect the genetic structure of Toxoplasma gondii isolated from various organs of sheep and cattle in the north of Iran. Convetional PCR was carried out by B1 and REP-529 genes of T. gondii. Nested and RFLP-PCR were performed for all positive samples using SAG2 and GRA6 genetic markers. Amplicons from second round of nested-PCR were sequenced and analyzed with NCBI database. Among of 179 examined samples, 38(21.20%) were positive. The highest of positive cases were found in kidney (28.60%). PCR-RFLP of SAG2 and GRA6 genes demonstrated the alleles of clonal type III in the all of isolates. Sequence analysis of the amplicons revealed the alleles of clonal type III and atypical isolates (Tg-67, Tg-100 and Tg-106). Phylogenetic analyses showed separate clade for the atypical isolates from others in the present study and the reference strains clades. In conclusion, the genetic characterization of T. gondii isolates from sheep and cattle showed high genetic diversity compared with standard type I, II and III genotypes. These results support the hypothesis of the existence of polymorphic and overlapping strains within livestock in Iran. It also suggested the necessity of increased genotyping and sampling efforts to accurately estimate T. gondii intra specific genetic diversity
Microbiology
Ali Gilani; Vadood Razavilar; Nordahr Rokni Rokni; Ebrahim Rahimi
Volume 8, Issue 1 , March 2017, , Pages 75-80
Abstract
Foods with animal origins play a substantial role in the transmission of Helicobacter pylori. The present investigation was carried out to study the vacA and cagA genotypes status of H. pylori isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. ...
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Foods with animal origins play a substantial role in the transmission of Helicobacter pylori. The present investigation was carried out to study the vacA and cagA genotypes status of H. pylori isolated from various types of meat samples. Two hundred and twenty meat samples were collected and cultured. H. pylori-positive strains were analyzed for the presence of vacA and cagA genotypes. Eleven out of 220 (5.00%) samples were positive for H. pylori. Findings were confirmed by nested PCR. Prevalence of H. pylori in the meat samples of slaughterhouses and butcheries were 72.20% and 27.70%, respectively. The most commonly detected genotypes in the meat samples of slaughterhouses and butcheries were vacA m1a (66.66%) and vacA s1a (37.50%), respectively. The S1am1a was the most commonly detected genotype. Meat sampled from butcheries had the higher prevalence of H. pylori and its genotypes than those of slaughterhouses (p < 0.05). Results showed that meat samples could be the potential sources of virulent strains of H. pylori. Application of sanitary measures in the storage, transportation and sale of meat is essential for reducing the levels of H. pylori cross contamination.
Vaishali Ghormade; Ankur Khare; R.P.S. Baghel
Volume 2, Issue 3 , September 2011, , Pages 147-155
Abstract
Nutrigenomics applies genomic technologies to study how nutrients affect expression of genes. With the advent of the post genomic era and with the use of functional genomic tools, the new strategies for evaluating the effects of nutrition on production efficiency and nutrient utilization are becoming ...
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Nutrigenomics applies genomic technologies to study how nutrients affect expression of genes. With the advent of the post genomic era and with the use of functional genomic tools, the new strategies for evaluating the effects of nutrition on production efficiency and nutrient utilization are becoming available. Nutrigenomics plays an efficient role in various fields of animal health like nutrition, production, reproduction, disease process etc. Nutrigenomic approaches will enhance researchers‟ abilities to maintain animal health, optimize animal performance and improve milk and meat quality.
