Poultry
Seyed Sattar Jalali; Alireza Talebi; Manoochehr Allymer; Ali Soleimanzadeh; Mazdak Razi
Volume 10, Issue 2 , June 2019, , Pages 139-144
Abstract
Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects ...
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Fertility is one of the most important parameters in breeder farms and cockerels play an outstanding role in fertility of eggs in broiler breeder units. Todays, supplementation of feed-additives such as organic selenium is used to increase fertility. The aim of this study was to evaluate the effects of different levels of Nano-Selenium (Nano-Se) on expression of molecular markers of spermatogonial stem cells (SSCs) in testis of broiler breeder males. A total of 30 roosters of 40 weeks age were randomly divided into 5 groups. Groups were as follow: 1) control (normal diet), 2) diet supplemented with 0.3 mg/kg sodium Selenite, 3) diet supplemented with 0.15 mg/kg Nano-Se, 4) diet supplemented with 0.3 mg/kg Nano-Se and 5) diet supplemented with 0.6 mg/kg Nano-Se. At the end of experimental period, birds autopsied and samples from testis of all birds were taken. The samples were used to examine the β1-integrin (CD29), thy-1(CD90 and NANOG mRNA expression by quantitative Real-Time PCR. The results of this study showed that testis of the groups fed with diets supplemented with 0.6mg/kg and 0.15mg/kg of Nano-Se had the highest and lowest mRNA expression of SSCs markers, respectively. In conclusion, the present study indicated that Nano-Se had advantages to sodium Selenite and 0.6 mg/kg of Nano-Se supplemented in males' diet in broiler breeders farms may contributes to optimal fertility via increasing mRNA expression of SSCs markers of roosters' testis and could be used to delay reduction of fertility caused by aging in broiler breeder males.
Poultry
Amir Hossein Asl Najjari; Zolfaghar Rajabi; Mehdi Vasfi Marandi; Gholamreza Dehghan
Volume 6, Issue 3 , September 2015, , Pages 227-231
Abstract
Influenza is a contagious viral disease that is seen in avian, human and other mammals, so its control is important. Vaccination against influenza virus subtype H9N2 is one of the ways in controlling program, for this reason several vaccines has been produced. Recently, application of inactivated oil-emulsion ...
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Influenza is a contagious viral disease that is seen in avian, human and other mammals, so its control is important. Vaccination against influenza virus subtype H9N2 is one of the ways in controlling program, for this reason several vaccines has been produced. Recently, application of inactivated oil-emulsion vaccines in poultry for controlling low pathogenic avian influenza is increasing. At present, oils that are used as adjuvant in commercial vaccines are mineral oils, which not only lack immunizing effect, but also produce some detriments. The aim of this study is the evaluation the immunogenicity of vegetable oils, which are more metabolizable and safer than mineral oils. In this study the efficacy of hexanic extracts of fig (Ficus carica) and olive (Olea europaea) fruit and also nano-selenium on the immunogenicity of the inactivated avian influenza virus subtype H9N2 was evaluated in broiler chickens. The results indicated that the prepared emulsions could elicit a little degree of immunity, but they could not inhibit the anamnestic response and infection. With regard to the results, it seems that the intact mixture of fig and olive fruit hexanic extracts could not be administered as an immunoadjuvant in the vaccine, and about nano-selenium. In spite of positive effect on the immunogenicity of avian influenza virus subtype H9N2, it still needs more work.
