Hülya Kara; Derviş Özdemir; Zekeriya Özüdoğru; Hülya Balkaya
Volume 14, Issue 6 , June 2023, , Pages 341-345
Abstract
Syrinx is a voice device and shows structural and functional differences between bird species. This study aimed to investigate morphological and histological structures of the syrinx in chukar partridge (Alectoris chukar) and Japanese quail (Coturnix coturnix japonica). In the present study, 12 male ...
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Syrinx is a voice device and shows structural and functional differences between bird species. This study aimed to investigate morphological and histological structures of the syrinx in chukar partridge (Alectoris chukar) and Japanese quail (Coturnix coturnix japonica). In the present study, 12 male chukar partridges and 12 male Japanese quail were used. The syrinx tissues were photographed by digital camera and fixed in formaldehyde solution. Five syrinxes were stained with methylene blue to make the syrinx rings distinct. After anatomical examination, tissues were passed through alcohol series, cleaned in xylene, and embedded in paraffin blocks. The blocks were cut and obtained sections were stained with Crossman modified triple staining and examined under camera attached light microscope. The syrinx of chukar partridges and Japanese quail consisted of cartilaginous tracheasyngeales and bronchosyngeales in the region of bifurcatio trachea and at the level of basis cordis. The tracheal rings constituting syrinx were counted three in chukar partridge and four in Japanese quail. The bronchial rings comprising syrinx counted nine in chukar partridge and eight in Japanese quail. In the histological examination, the pesullus structure was hyaline cartilage and calcificated with increasing ages being covered by pseudostratified columnar epithelium. The results of the study suggested that chukar partridge and Japanese quail syrinxes have some morphological differences compared to the other bird species; but, anatomically and histologically similarities to many bird species.
Anietie Francis Udoumoh; Innocent Chima Nwaogu; Udensi Maduabuchi Igwebuike; Ikechukwu Reginald Obidike
Volume 13, Issue 3 , September 2022, , Pages 301-308
Abstract
The developmental morphology of the bursa of Fabricius (BF) of broiler chicken was evaluated in this study using gross anatomical, histological, reticulin histo-chemical, and transmission electron microscopic techniques. The result showed that the short plica of the BF at embryonic day (ED) 14 was a ...
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The developmental morphology of the bursa of Fabricius (BF) of broiler chicken was evaluated in this study using gross anatomical, histological, reticulin histo-chemical, and transmission electron microscopic techniques. The result showed that the short plica of the BF at embryonic day (ED) 14 was a mesenchymal tissue that contained cells, including mesenchymal cells and lymphoblasts. The organo-somatic index (OSI) of the BF peaked at ED 17, while the BF of ED 19 showed well delineated follicle-associated and inter-follicular epithelial (IFE) cells. Whereas, the IFE contained apical vacuoles which increased in size with age, the BF contained lymphoid follicles which were first observed at ED 17, and increased steadily in mean diameter from ED 19 to post-hatch day (PD) 28. The framework of the inter-follicular areas and the follicular capsules were composed of collagen type III fibres. Moreover, the cortico-medullary basement membranes were well established with peripheral and cortico-medullary basement capillaries becoming very consistent between PDs 5 and 7. The lymphoid follicle contained lymphocytes with mitotic figures, and plasma cells which showed extensive network of rough endoplasmic reticulum at PD 21, while macrophages contained increasing evidence of phagocytosis from PDs 35 to 56. In conclusion, the morphological features of the BF of broiler chicken in this study reveals that the key function of B-cell maturation within the BF may occur between the first 3 to 4 weeks after hatching, and thereafter, may primarily produce immunoglobulins until involution occurs.
Ali Al-Ghamdi; Kareem Morsy; Saad Dajem; Ali Shati; Mohammed Al-Kahtani; Ahmed Baiomy; Ahmed Ezzat
Volume 12, Issue 1 , March 2021, , Pages 15-24
Abstract
During a recent survey on digenean parasites infecting marine fish captured from water located along the Red Sea off Gizan Coasts, Saudi Arabia, thirty-two (29.09%) out of 110 fish were naturally infected with three different species of digenean parasites. The taxonomical status of the recovered worms ...
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During a recent survey on digenean parasites infecting marine fish captured from water located along the Red Sea off Gizan Coasts, Saudi Arabia, thirty-two (29.09%) out of 110 fish were naturally infected with three different species of digenean parasites. The taxonomical status of the recovered worms was confirmed by light microscopy and molecular analysis. Transversotrema licinuminfecting the haffara sea bream Rhabdosargus haffara possessed a transversely elongated body with eyespots and the anterior body margin with delineated velum along with its entire breadth. Oral sucker was absent, and eyespots were paired anterior to or at the level of ventral sucker. Phyllodistomum hoggettaefrom the urinary bladder of the Arabian Nagil Plectropomus areolatus was differentiated by its elongated body, narrow anterior end widened as going posteriorly with prominent marginal undulations in the hindbody. Gyliauchen volubilisinfecting the marbled spinefoot Siganus rivulatus, was with fleshy body, conical, tapered anteriorly, convex dorsally, and concave ventrally. Prepharynx was very long and convoluted and had occupied the greater part of the anterior half of the body. Multiple sequence alignment and phylogeny were based on maximum likelihood analysis for the ITS–2 and 28S rDNA for Transversotrema licinum and Phyllodistomum hoggettae, respectively, were constructed. They were compared to species within their families and further deposited into the Genebank under accession numbers MG 722711 and MG722710, respectively.
Histology
Mohammad Hamed Shahsavari; Gholamali Moghaddam; Hossein Daghigh Kia; Ana Paula Ribeiro Rodrigues
Volume 10, Issue 1 , March 2019, , Pages 9-16
Abstract
Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic ...
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Previous studies have reported many discrepancies about the best type and concentration of cryoprotective agents (CPAs) and biological variability among various pre-antral follicle classes after cryopreservation of ovarian tissue. The aim of this study was to investigate the impacts of some synthetic polymers on histological characteristics of different types of pre-antral follicles after bovine ovarian tissue vitrification. From each bovine ovarian pair, fragments were recovered and immediately fixed for analysis (fresh control group) or submitted to vitrification (sucrose, X-1000, Z-1000 and polyvinylpyrrolidone groups), either followed by in vitro culture for 1 or 5 days. In this case, although, the addition of X-1000 resulted in greater percentages of normal follicles for almost all pre-antral follicle classes compared to those of other groups, there are some exceptions. These results indicate that the inclusion of polyvinylpyrrolidone in the freezing media can improve the morphology of the post-warmed transitional follicles and cultured primordial follicles on day five more than other CPAs. According to the results of this study, it can be concluded that although ovarian tissue cryopreservation is often performed to preserve the primordial follicles, by choosing the best combination of permeating and non-permeating CPAs (synthetic polymers), more advanced stages of bovine pre-antral follicles, transitional, primary and secondary follicles, may also survive the cryopreservation process.
Sayedeh Zahra Mozafari; Abdolhossein Shiravi; Fatemeh Todehdehghan
Volume 3, Issue 2 , June 2012, , Pages 119-123
Abstract
Reproductive parameters evaluation is considered as helpful tool for gene bank formation in ecological and economically important animals species. Gloydius halys caucasicus is venomous, viviparous pit viper of northwest of Iran. In this research, the spermatic reproductive parameters of this taxon were ...
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Reproductive parameters evaluation is considered as helpful tool for gene bank formation in ecological and economically important animals species. Gloydius halys caucasicus is venomous, viviparous pit viper of northwest of Iran. In this research, the spermatic reproductive parameters of this taxon were studied. Twenty six male snakes were collected from Takht-e-Soleiman region between September and October, 2010. Findings revealed that male snakes with body length of 45.07 ± 2.83 cm and body weight of 51.50 ± 10.42 g, and right and left gonads volume of 0.12 ± 0.03 mL and 0.06 ± 0.01 mL are mature ones and sperms concentration in first, middle and final regions of vas deferens duct were, 22.30 ± 19.34 ×106 mL-1, 30.34 ± 11.55 ×106 mL-1, and 37.65 ± 16.46×106 mL-1, respectively. The sperms motility at three regions of duct were 60.53%, 62.07%, and 60.00% and percentage of immotile sperms in these regions were 39.46%, 37.92%, and 39.84%, respectively. Percentage of morphologically normal sperms was 69.23 ± 10.57% and abnormal sperms was 30.76 ± 10.57%; including 12.69 ± 5.25% spiral tailed, 7.33 ± 4.37% coiled tailed and 4.16 ± 2.51% folded tailed sperms. Percentage of live sperms in the first, middle and final regions of duct were 55.76 ± 10.77%, 58.84 ± 12.77%, and 57.69 ± 9.91%, respectively and percentage of dead sperm in these regions were 44.23 ± 10.77%, 41.15 ± 12.77%, and 42.30 ± 9.91%, respectively. Results suggested, mature sperms with acceptable reproductive quality could be collected from Gloydius halys caucasicus snake of Iran between September and October.
Mohammad Shadkhast; Homayoun-Reza Shabazkia; Amin Bigham-Sadegh; Seyed Ebrahim Shariati; Taji Mahmoudi; Mojdeh Shariffian-Fard
Volume 1, Issue 3 , December 2010, , Pages 134-141
Abstract
In this study morphological characterization of leukocyte and erythrocyte were investigated in central Asian tortoise (Testudo horsfieldii) in Iran. 7 males and 7 females were used in this study. Erythrocyte and leukocyte morphology were described using Wright´s technique. The sizes of erythrocyte ...
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In this study morphological characterization of leukocyte and erythrocyte were investigated in central Asian tortoise (Testudo horsfieldii) in Iran. 7 males and 7 females were used in this study. Erythrocyte and leukocyte morphology were described using Wright´s technique. The sizes of erythrocyte and their nuclei were measured using an ocular micrometer under objective lenses (100x). Results of this study were compared with previous work on the other reptile species. Because there were no significant differences between the erythrocyte size of female and male tortoise, the data from the females and males of individual species were pooled. The mean length of mature erythrocytes was 1.98 µm (± 0.16 SD, with a range 1.10 - 2.50 µm).The mean width of mature erythrocytes was 1.10 µm (± 0.14 SD, with a range of 0.80 - 1.90 µm).The mean length of nucleus was 0.71 µm (± 0.09 SD, with a range of 0.50 - 1.00 µm). The mean width of the nucleus was 0.52 µm (± 0.07 SD, with a range of 0.40 - 0.70µm). Five types of leukocytes were found in the tortoise blood namely basophiles, eosinophils, lymphocytes, heterophils and monocytes.