Azadeh Yektaseresht; Zahra Hemati; Amir Arsalan Khorsand; Shoor Virsingh
Volume 13, Issue 4 , December 2022, , Pages 603-606
Abstract
No diagnostic kits and reagents are available in the market to detect and evaluate camel immune responses to different pathogens. This study aimed to produce sheep anti-camel (Camelus dromedarius) polyclonal antibodies (pAbs) and to determine the specificity with other species immunoglobulin. Immunoglobulins ...
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No diagnostic kits and reagents are available in the market to detect and evaluate camel immune responses to different pathogens. This study aimed to produce sheep anti-camel (Camelus dromedarius) polyclonal antibodies (pAbs) and to determine the specificity with other species immunoglobulin. Immunoglobulins (Igs) from camel serum samples were purified using ammonium sulfate precipitation (40.00% saturated ammonium sulfate). Purity of the camel Igs was tested by sodium dodecyl sulfate polyacrylamide gel electrophoresis. PAbs against (Camelus dromedarius) immunoglobulins were generated by immunizing sheep with purified Igs. Anti- camel Ig polyclonal antibodies titer and specificity were determined using ELISA and Western blot techniques. Polyclonal antibodies specific to camel Igs were significantly high in immunized sheep which confirmed the immunization procedure. PAbs reacted specifically with camel serum immunoglobulin and did not react with other species immunoglobulin of horse and chickens. Polyclonal antibodies produced in this study can be regarded as a valuable tool to be used for immune-diagnostic purposes in camel population world-wide.
Pwaveno Huladeino Bamaiyi; Arunsi Ukairu Kalu
Volume 2, Issue 4 , December 2011, , Pages 278-281
Abstract
One hundred and five (105) Camels were investigated at the Maiduguri abattoir, Nigeria using floatation and sedimentation techniques for helminth parasites and haematological indices with the microhaematocrit reader. Overall, prevalence of infection was 92.4% [(Coccidia (8.5%), Strongyloides (8.5%), ...
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One hundred and five (105) Camels were investigated at the Maiduguri abattoir, Nigeria using floatation and sedimentation techniques for helminth parasites and haematological indices with the microhaematocrit reader. Overall, prevalence of infection was 92.4% [(Coccidia (8.5%), Strongyloides (8.5%), Trichuris (11.4%), Ciliates (6.7%), Ascaris sp. (3.8%), Moniezia sp. (1.9%), Amphistome sp. (0.9%) and Balantidium sp. (0.9%)]. There was no significant difference between infected and non-infected camels for blood parameters. There is need to regularly deworm camels and further study the impact of helminthes in the camel especially with respect to its zoonotic potentials in countries with significant population of camels.
Saeed Nazifi; Amin Tamadon; Mohammad-Amin Behzadi; Shahram Haddadi; Ali-Reza Raayat-Jahromi
Volume 2, Issue 2 , June 2011, , Pages 135-138
Abstract
The economic importance of tick infestation on camels are important as they are important meat and milk producer animals in the less vegetation area of Iran and their health and production are greatly affected by the high tick infestation. In this investigation, tick infestations on camels (Camelus ...
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The economic importance of tick infestation on camels are important as they are important meat and milk producer animals in the less vegetation area of Iran and their health and production are greatly affected by the high tick infestation. In this investigation, tick infestations on camels (Camelus dromedarius) were determined in Qeshm Island, Iran. A total number of 912 adult ticks (472 males and 440 females) were collected and identified. Hyalomma dromedarii was the predominant tick specie and accounted for 61.9% of the adult ticks. Other hard ticks were H. anatolicum excavatum (22 %), H. asiaticum asiaticum (14.2 %), H. marginatum (1.9 %), H. impeltatum (0.4 %) and Ripicephalus bursa (0.4 %). In conclusion, The provision of tick control programs in the Qeshm Island would seem a prerequisite for improving camel meat and milk production.
