Theriogenology
Siamak Kazemi-Darabadi; Reza Asadpour; Amir Ali Shahbazfar; Sajjad Alizadeh
Volume 10, Issue 2 , June 2019, , Pages 125-132
Abstract
Testicular torsion is a consequence of spermatic cord twisting which causes progressive damage to the structure of the testis and reduces sperm quality and usually results in infertility. In the present study, with the assumption of the protective effects of L-carnitine and betamethasone against ischemia-reperfusion ...
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Testicular torsion is a consequence of spermatic cord twisting which causes progressive damage to the structure of the testis and reduces sperm quality and usually results in infertility. In the present study, with the assumption of the protective effects of L-carnitine and betamethasone against ischemia-reperfusion (IR) injuries, their effects on twisted testicles were evaluated and compared. Twenty Wistar rats were randomly divided into four groups and used in this study. Except for the Sham (S) group, testicular IR was induced surgically in three other groups, including Control (C), Betamethasone (BM), and L-carnitine (LC) groups. Betamethasone and L-carnitine were injected before detorsion in the BM and LC groups, respectively. After twelve hours of reperfusion, the testicles were detached, and prepared for sperm parameters evaluation such as sperm count, motility, viability, morphology, and chromatin quality, and histopathologic evaluations, including mean seminiferous tubular diameter (MSTD), germinal epithelial cell thickness (GECT), and Johnsen’s mean testicular biopsy scoring (MTBS). The MSTD, GECT, and healthy sperms in the C group were significantly lower than the other groups, while the BM and LC groups were significantly different from others in MTBS. The number of sperms and sperm motility in the BM group was significantly higher than the C group. Sperm viability in the BM and LC groups were significantly higher than the C group. The results of this study showed that both L-carnitine and betamethasone similarly can be effective in treating testicular IR injuries.
Mazdak Razi; Hassan Malekinejad; Rajab-Ali Sardkhanlou; Farshid Sarrafzadeh-Rezaei
Volume 2, Issue 3 , September 2011, , Pages 189-201
Abstract
This study was designed to clarify bilaterally effect of long-time varicocele on left and right testes and to identify the effect of varicocele on maturation divisions during spermatogenesis. Moreover, the percentage of seminiferous tubules (STs) with I-VIII and IX-XIV stages of spermatogenesis and sperm ...
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This study was designed to clarify bilaterally effect of long-time varicocele on left and right testes and to identify the effect of varicocele on maturation divisions during spermatogenesis. Moreover, the percentage of seminiferous tubules (STs) with I-VIII and IX-XIV stages of spermatogenesis and sperm parameters such as sperm motility and abnormality types were evaluated. The left varicocele was induced in test group (n=18) and control-sham animals (n=6) were gone under simple laparatomy. The test group further sub-divided into three subgroups based on the duration of varicocele (4, 6 and 8 months after varicocele induction). The histological alterations, spermiogenesis index (SPI), Sertoli cells (SCs), cytoplasmic lipid and alkaline phosphatase (ALP), germinal cells first and second maturation divisions, spermatogenesis stages, natural mating and sperm characteristics (count, motility and abnormality) were evaluated. Histological results showed that the varicocele-induced damage affected both stages of I-VIII and IX-XIV and up to 20% of the STs exhibited negative SPI and SCs degeneration. After 8 months, majority of STs demonstrated a remarkable reduction in the first and second maturation division in both of the testes. Nevertheless, the right testicles were manifested with significantly (P < 0.05) lower detrimental features in comparison with the left ones. Following varicocele induction the sperm count, viability and motility reduced time-dependently. Our data suggested that monolateral varicocele-induction affected both right and left testicles simultaneously albeit with differences. The varicocele-induced injuries were manifested as negative SPI and SCs degeneration, which may have led to poor sperm quantity and quality.
