Seyed Hamid Farrokhifar; Ramezan Ali Jafari; Naeem Erfani Majd; Seyed Reza Fatemi Tabatabaee; Mansour Mayahi
Volume 4, Issue 4 , December 2013, , Pages 221-225
Abstract
The effects of dietary vitamin E levels on mucosal maltase and alkaline phosphatase (ALP) enzyme activities and on the amount of mucosal malonyldialdehyde (MDA) in broiler chickens were studied in the present study. One hundred and eighty of day old male broiler chicks (Ross 308 strain) were randomly ...
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The effects of dietary vitamin E levels on mucosal maltase and alkaline phosphatase (ALP) enzyme activities and on the amount of mucosal malonyldialdehyde (MDA) in broiler chickens were studied in the present study. One hundred and eighty of day old male broiler chicks (Ross 308 strain) were randomly assigned into five groups, each with three replicates and 12 chicks in each replicate. Chickens in group A were fed corn-soy- based diet, while those in groups B, C, D and E were fed the same diet with 20, 60, 180, and 540 mg kg-1 vitamin E supplement (d-alpha tocopherol), respectively. Six birds were randomly chosen from each group, and were euthanized on days 10, 21, 32, and 42 of age. One segment of small intestine outset was homo-genized and mucosal ALP and maltase activity were measured. Moreover, mucosal lipid peroxidate amount was measured to reveal the impact of vitamin E on oxidative stress. Maltase activity was increased with the increase of vitamin E up to 60 mg kg-1 of diet while with further levels, it was decreased. Addition of 60 mg kg-1 of vitamin E to the diet significantly increased ALP enzyme activity (p ≤ 0.001). Addition of 540 mg kg-1 of vitamin E supplement to the diet led to the minimum amount of MDA at 32 days of age. It may be concluded that supplementation of broiler's diet with 60 mg kg-1of vitamin E can increase mucosal maltase and ALP enzyme activity.
Ehsan Hosseini; Saeed Nafisi; Samad Zare
Volume 2, Issue 4 , December 2011, , Pages 222-225
Abstract
The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the variations of corticosterone, Free-T3, Free-T4 and malonyl dialdehyde in plasma in 48 adult white New Zealand male rabbits. Animals were divided into six groups namely, C1 (normal diet, not ...
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The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the variations of corticosterone, Free-T3, Free-T4 and malonyl dialdehyde in plasma in 48 adult white New Zealand male rabbits. Animals were divided into six groups namely, C1 (normal diet, not exposed), C2 (normal diet, sham exposed), T1 (normal diet, exposed to electromagnetic field), C3 (high-cholesterol diet, not exposed), C4 (high-cholesterol diet, sham exposed) and T2 (high-cholesterol diet, exposed to electromagnetic field). In eight separate experiments, sham exposed groups (C2 and C4), were exposed to sham stimulated (without electromagnetic stimulation) for 5 days, 2 hour/day and the rabbits of the treatment groups (T1 and T2) were treated with triangular form 10 Hz of electromagnetic field for 5 days, 2 hour/day, while the control groups (C1 and C3) had no any exposure. At the end of the exposure, after a 12- hour fasting period, blood samples were taken and level of corticosterone, Free-T3 and Free-T4 were measured by Elisa kits and level of malonyldialdehyde was measured by spectrophotometric method. The results indicated that the blood serum levels of Free-T3, Free-T4 and Corticosterone in the T1 and T2 groups were significantly increased compared to those of their own control groups (P < 0.05). Malonyldialdehyde levels in T2 animals showed a significant decrease compared to that of animals of C3 and C4 (P < 0.05). We conclude that 10 Hz pulsed electromagnetic field can alter the levels of Free-T3, Free-T4 and corticosterone in animals with both normal diet and hyperchlosterol diet and also alter the amount of malonyldialdehyde in animals with hyperchlosterol diet.
