Maryam Nazarbeigy; Mohammad Yakhchali; Fazel Pourahmad
Volume 12, Issue 2 , June 2021, , Pages 229-233
Abstract
Parasitic nematodes of ovine abomasum are of economic and hygienic importance throughout the world and Iran. This study was aimed to evaluate molecular identity and species diversity of parasitic nematodes in the abomasum of slaughtered sheep in Ilam, Iran. In this study, a total number of 240 of abomasa ...
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Parasitic nematodes of ovine abomasum are of economic and hygienic importance throughout the world and Iran. This study was aimed to evaluate molecular identity and species diversity of parasitic nematodes in the abomasum of slaughtered sheep in Ilam, Iran. In this study, a total number of 240 of abomasa were randomly collected from the slaughtered sheep at industrial slaughterhouses in Ilam in all seasons between 2017 and 2018. The abomasum content and abomasal mucosa were removed and washed. The collected nematodes were morphologically identified. The genomic DNA was extracted and a 300 bp-fragment-length from internal transcribed spacer 2 ribosomal ribonucleic acid (ITS2-rRNA) gene was amplified. Overall prevalence was 66.70% (160/240). Five species of four genera of nematodes including Marshallagia marshalli (43.70%), Ostertagia circumcincta (15.50%), Parabronema skrjabini (5.00%), M. occidentalis (2.50%), and Haemonchus contortus (0.04%) were identified. Ostertagia circumcincta and H. contortus were found to be different in two nucleotides. There was no nucleotide difference between M. marshalli and M. occidentalis. This study revealed a significant prevalence of parasitic nematodes in sheep abomasum and species diversity of Trichostrongylid nematodes in the region.
Soraya Naem; Tahmineh Gorgani
Volume 2, Issue 4 , December 2011, , Pages 238-241
Abstract
The present study was carried out to determine parasitic infection of sheep with gastrointestinal helminthes in a slaughterhouse in Fereidoonkenar city, Iran. A total number of 50 sheep were examined from April to September 2008. Nematodes were removed from abomasums, small and large intestines, and ...
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The present study was carried out to determine parasitic infection of sheep with gastrointestinal helminthes in a slaughterhouse in Fereidoonkenar city, Iran. A total number of 50 sheep were examined from April to September 2008. Nematodes were removed from abomasums, small and large intestines, and kept in A.F.A. solution (alcohol, formaldeyde, acetic acid, distilled water, and glycerine) for further diagnosis. Cestodes were removed from small intestine, washed with water, and stained with carmine acid. The results of this study indicated that 70% of examined animals were infected as follows: Ostertagia circumcincta and Marshallagia marshalli (38%), Trichostrongylus colubriformis (16%), Nematodirus spathiger (14%), Skrjabinema ovis (12%), Haemonchus contortus (10%), Camelostrongylus mentolatus (4%), and Gongylonema pulchrum, Cooperia punctata, Bunostomum trigonocephalum, Chabertia ovina (2%). Among examined animals, 14% infected with Moniezia expansa, 10% with Avitellina centripunctata and 2% with Helicometra giardi. The infection rate in younger animals was higher than in adults. The maximum infection rate was with O. circumcincta and M. marshali. No infection was found in examined rumens.
