Pharmacology
Abbas Ahmadi; Saleh Bamohabat Chafjiri; Rajab Ali Sadrkhanlou
Volume 8, Issue 4 , December 2017, , Pages 281-286
Abstract
Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. Satureja khuzestanica has antioxidant effects. The aim of this study was to examine whether S. khuzestanica essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four ...
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Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. Satureja khuzestanica has antioxidant effects. The aim of this study was to examine whether S. khuzestanica essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four adult female mice were divided into six groups including dimethyl sulfoxide (control), SKEO 225.00 mg kg-1 (orally), busulfan 3.00 mg kg-1 (orally), busulfan 36.00 mg kg-1 (intraperitoneally), busulfan 3.00 mg kg-1 and SKEO and busulfan 36.00 mg kg-1 and SKEO. After 28 days, the mice were euthanized and oocytes were removed for in vitro fertilization (IVF) rate evaluation. Oocyte quantity and quality, fertilization rate and pre-implantation embryo development were daily examined with a stereo microscope in a period of 120 hr. Serum levels of estradiol and progesterone were also evaluated. Busulfan caused significant decreases in oocyte number and quality, fertilization rate, pre-implantation embryo development and embryo quality. The SKEO significantly decreased the adverse effects of busulfan. The present study indicated that SKEO can protect female fertility potential against busulfan induced damages.
Mohadeseh Panahi; Saeideh Keshavarz; Farhad Rahmanifar; Amin Tamadon; Davood Mehrabani; Negar Karimaghai; Masood Sepehrimanesh; Heydar Aqababa
Volume 6, Issue 4 , December 2015, , Pages 273-278
Abstract
The aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. Three groups of male adult rats were used in this study. The first group was injected by single dose of busulfan (10 mg kg-1) and their testes were removed on day 35 post injection. ...
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The aim of the present study was stereological evaluation of testes of azoospermic animal model using busulfan in rat. Three groups of male adult rats were used in this study. The first group was injected by single dose of busulfan (10 mg kg-1) and their testes were removed on day 35 post injection. The second group received double doses of busulfan with 21 days interval and their testes were removed on day 35 after the second injection. The testes of the third group were removed without busulfan therapy. In 10 circular transverse sections of tubules stained with hematoxylin-eosin, stereological parameters were measured. The testes were rated for its spermatogenic potential on a modified spermatogenic scale of 0 to 6. Cellular (germinal epithelium) diameter and area of the seminiferous tubules, total diameter and cross sectional area of the tubules of the seminiferous tubules in rats that received double doses of busulfan were less than the rats in single dose of busulfan and control groups (p < 0.05). Spermatogenesis index of seminiferous tubules in rats receiving two doses of busulfan was less than the rats received one dose of busulfan (p < 0.001) and the index of both treatment groups were less than the control group (p < 0.001). In conclusion, two doses of busulfan injection with 21 days interval produced an appropriate experimental model of induced azoospermia with comparable stereological indices of seminiferous tubules in rat.
Maryam Ansari-Lari; Mehdi Mohebbi-Fani; Abbas Rowshan-Ghasrodashti
Volume 3, Issue 4 , December 2012, , Pages 233-237
Abstract
This study was designed to investigate causes of culling in industrial dairy herds in Fars province and to describe the pattern of reason-specific culling with respect to age of animal and interval from calving to culling. A total number of 9 dairy herds were selected for the study and information about ...
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This study was designed to investigate causes of culling in industrial dairy herds in Fars province and to describe the pattern of reason-specific culling with respect to age of animal and interval from calving to culling. A total number of 9 dairy herds were selected for the study and information about culling reasons, birth date, last calving date and culling date was collected for culled cows during 2005- 2006. Infertility (32.6% of all culls) was the most prevalent reason of culling followed by mastitis (6.5%). The time interval from last calving to culling averaged 240 days (SD = 176) and nearly 28% of cows were culled in the first 100 days after calving. Mean age of animals at culling was 6 years (SD = 2.7) and median was 5.7 years. In Cox proportional hazard model for calving to culling interval, infertility (hazard ratio [HR] = 0.26) showed lower risk whereas mastitis (HR = 2.40), left displaced abomasum (HR = 2.60) and peripartum problems (HR = 2.60) had higher risk of culling compared with voluntary cull. In the Cox model for age at culling, risk of culling was significantly higher for infertility (HR = 1.70), left displaced abomasum (HR = 3.15), and peripartum problems (HR = 2.10) compared with voluntary culling. In conclusion, farmers tend to keep infertile cows for longer period from calving to culling while infertile cows are generally culled at younger age. Also, early culling appeared to have a high proportion of culls in the studied herds.
