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Immunoprotection in sheep against Haemonchus contortus using its thiol-purified excretory/secretory proteins

Selvarayar Arunkumar

Volume 3, Issue 4 , December 2012, , Pages 239-244

Abstract
  Excretory/Secretory antigen was prepared by culturing live adult worms of Haemonchus contortus in RPMI 1640 medium at a concentration of 50 worms per mL in a culture-flask at 37 ˚C for 24 hr and the culture supernatant was used as antigen. The E/S antigen was purified by thiol-sepharose affinity chromatography. ...  Read More