Fish & Aquatic
Zohre Khorshidi; Kourosh Sarvi Moghanlou; Ahmad Imani; Shahryar Behrouzi
Volume 14, Issue 10 , October 2023, , Pages 567-573
Abstract
This research explored the impacts of feed-born silver nanoparticles (AgNPs) on common carp (Cyprinus carpio) and whether dietary curcumin supplementation could ameliorate the impacts of AgNPs on growth, hemato-biochemical parameters and digestive enzyme activity. Nine experimental diets were prepared ...
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This research explored the impacts of feed-born silver nanoparticles (AgNPs) on common carp (Cyprinus carpio) and whether dietary curcumin supplementation could ameliorate the impacts of AgNPs on growth, hemato-biochemical parameters and digestive enzyme activity. Nine experimental diets were prepared containing 0.00, 0.05, and 0.15 g kg-1 AgNPs, as well as 0.00, 0.75, and 1.50 g kg-1 curcumin in a factorial design. Triplicate groups of common carp (4.82 ± 0.41 g) were fed on the test diets for 60 days. The results demonstrated that AgNPs reduced growth performance and enhanced the feed conversion ratio dose-dependently. Supplementing 0.75 g kg-1 curcumin at a low AgNP level improved the growth rate, while its inclusion at a high AgNP level led to further suppression of growth performance. The highest hematocrit value, hemoglobin concentration and white blood cell count were recorded in the group receiving 0.75 g kg-1 curcumin. Serum glucose, cholesterol and triglyceride concentrations were elevated by increasing AgNP levels. However, curcumin inclusion, particularly at the lower level of AgNPs significantly decreased their values. Similarly, intestinal alkaline protease and lipase activities were progressively reduced by increasing dietary AgNP contents, but, significant improvements were observed by curcumin application at the lower AgNP level. Our results revealed that curcumin supplementation could limit the toxic effects of lower dietary AgNP contents.
Laleh Mavaddatiyan; Shiva Khezri; seyyed Meysam Abtahi Froushani
Volume 12, Issue 1 , March 2021, , Pages 47-52
Abstract
Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS). Previous studies have shown that myelin degradation during MS and EAE resulted in reduced expression of some of the proteins, e.g., the MBP (myelin basic protein), and increased expression of genes such as ...
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Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS). Previous studies have shown that myelin degradation during MS and EAE resulted in reduced expression of some of the proteins, e.g., the MBP (myelin basic protein), and increased expression of genes such as iNOS (Inducible nitric oxide synthase) and NOGO-A in the affected patients. In the present study, EAE was induced by immunizing Wistar rats (n=12) with homogenized spinal cord of guinea pig and Freund's complete adjuvant. Curcumin is an active ingredient in turmeric with anti-inflammatory properties, which has been studied in this article. In this study, the effect of curcumin administration on the change of the expression of MBP, NOGO-A, and iNOS genes was evaluated using the RT-PCR (Reverse transcription-polymerase chain reaction) technique. The obtained results indicated it could be concluded that curcumin was able to improve EAE by increasing the amount of MBP gene expression and reducing the intensity of NOGO-A expression.
Theriogenology
Parisa Aparnak; Adel Saberivand
Volume 10, Issue 3 , September 2019, , Pages 221-226
Abstract
Canine seminal plasma contains antioxidant enzymes to protect sperm against internally generated ROS. These enzymes are removed from seminal plasma during the process of cryopreservation. The freezing/thawing process can cause some morphological and functional changes via ice crystallization and osmolality ...
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Canine seminal plasma contains antioxidant enzymes to protect sperm against internally generated ROS. These enzymes are removed from seminal plasma during the process of cryopreservation. The freezing/thawing process can cause some morphological and functional changes via ice crystallization and osmolality imbalance. The present study was conducted to evaluate the effects of curcumin supplementation on sperm total count, motility, progressive motility, viability, morphology, total antioxidant capacity (TAC), DNA integrityand NOX5 gene expression of dog frozen semen. The pooled semen was allocated to fresh (Group 1) and frozen (Group 2) controls, curcumin (2.50 mM) (Group 3) and curcumin (5.00 mM), (Group 4). Sperm parameters including total sperm count, morphology, motility, progressive motility, sperm concentration and DNA integrity in addition to TAC were evaluated in fresh and frozen-thawed semen samples. Real-time PCR was used to investigate NOX5 and GADPH (reference gene) genes expressions. Curcumin at 2.50 mM provided a greater protective effect on the DNA integrity compared to 5.00 mM and control groups. TAC was significantly higher in 2.50 mM group than other groups. NOX5 gene expression in curcumin 2.50 mM was higher than 5.00 mM group. In conclusion, curcumin seems to emolliate sperm parameters and to protect sperm against sperm reactive oxygen stress and increases NOX5 gene expression.
Pathology
Firoozeh Niazvand; Layasadat Khorsandi; Mohammadreza Abbaspour; Mahmoud Orazizadeh; Negar Varaa; Mahtab Maghzi; Kheironesa Ahmadi
Volume 8, Issue 2 , June 2017, , Pages 155-161
Abstract
Curcumin has been found to be very efficacious against many different types of diseases. However, the major disadvantage associated with the use of curcumin is its low systemic bioavailability. In the present study the protective effects of curcumin-loaded poly lactic-co-glycolic acid nanoparticles (nanocurcumin) ...
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Curcumin has been found to be very efficacious against many different types of diseases. However, the major disadvantage associated with the use of curcumin is its low systemic bioavailability. In the present study the protective effects of curcumin-loaded poly lactic-co-glycolic acid nanoparticles (nanocurcumin) against mono-iodoacetate-induced osteoarthritis in rats was investigated. Mono-iodoacetate was injected into right knee joints to induce osteoarthritis. In experimental groups, 14 days after injection of mono-iodoacetate, curcumin (200 mg kg-1) and nanocurcumin (200 mg kg-1) were gavaged, respectively, for two weeks. Then the rats were sacrificed and the right knee joints were removed and fixated in 10% formalin for histological assessments. Cellularity and matrix staining were significantly increased in articular cartilage of curcumin-treated animals compared to mono-iodoacetate group (p < 0.01). These effects were significantly (p < 0.01) more in nanocurcumin-treated animals. These results suggested that administration of nanocurcumin prevented the structural changes of articular cartilage in mono-iodoacetate modelofosteoarthritis in rats.
Ali Soleimanzadeh; Adel Saberivand
Volume 4, Issue 3 , September 2013, , Pages 185-189
Abstract
Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, ...
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Reactive oxygen species (ROS) generation, induced by the cryopreservation process, can be responsible for mammalian sperm damage. Curcumin is known as an effective antioxidant against oxidative stress. The aim of this study was to evaluate the effects of curcumin on sperm count, motility and viability, semen total antioxidant capacity and DNA integrity of rat spermatozoa during semen freeze-thawing process. Sperm collected from 10 adult rats were divided into two groups (n=10 for each group): control and a test group supplemented with 2.5 mM curcumin. After freezing-thawing, the number of spermatozoa, motility, viability, total antioxidant capacity (TAC), and DNA integrity of the sperm were analyzed. Motility, viability and DNA integrity of sperm were significantly preserved in treatment groups compared to the control (p < 0.05) after freezing-thawing. Following cryopreservation, TAC was significantly preserved in thawing semen supplemented with curcumin compared to the control group (p<0.05). Based on our results, it is concluded that curcumin addition during freezing resulted in positive effects on sperm parameters after thawing in adult rats.