Laparoscopic left hemihepatectomy combined with right lateral hepatic lobectomy in pigs: surgical approach and comparative study of the inflammatory response versus open surgery
Hua
Zhang
Department of Animal Science, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China
author
Jin-jin
Tong
Department of Animal Science, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China
author
Zhao-Nan
Zhang
Department of Animal Science, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China
author
Hong-Bin
Wang
Department of Veterinary Surgery, College of Veterinary Medicine, Northeast Agricultural University, Harbin, China
author
Yong-Hong
Zhang
Department of Animal Science, College of Animal Science and Technology, Beijing University of Agriculture, Beijing, China
author
text
article
2021
eng
This study describes a left hemihepatectomy combined with a right lateral hepatic lobectomy. It compares the inflammatory response associated with laparoscopic hepatectomy (LH group, n = 7) with conventional open hepatectomy (OH group, n = 7). Blood was collected before surgery as well as 1, 2, 3, 5, and 7 days after surgery to determine the white blood cell count and levels of serum cortisol (COR), interleukin-6 (IL-6), and C-reactive protein (CRP). The left hemi-hepatectomy combined with a right lateral hepatic lobectomy was completed in miniature pigs. The average operative time was 139.00 ± 9.07 min, which was longer than that in the OH group (121.67 ± 3.02 min). The length of surgical incision associated with the OH group was 17.93 ± 1.09 cm, significantly longer than that related to the LH group (5.10 ± 0.17 cm). The estimated mean blood loss in the LH group was 136.43 ± 63.24 mL, which was significantly lower than that in the OH group. No severe complications (e.g., massive bleeding, bile leakage, and air embolism) were reported. The CRP levels, COR, and IL-6, increased significantly in the OH group and then slowly returned to their preoperative levels. A postoperative laparoscopic exploration revealed that the incised portion of the liver adhered to the omentum, but no additional abnormalities were observed. These findings indicate that a 4-trocar method for laparoscopic left hemihepatectomy combined with a right lateral hepatic lobectomy is safe and feasible. The inflammatory response for those receiving LH are lower than that for those receiving OH. This porcine model can be used as a research analog for liver disease and regeneration.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
1
6
https://vrf.iranjournals.ir/article_45639_ff61d7b6dbf128f9fc22cee2448090b6.pdf
dx.doi.org/10.30466/vrf.2019.105865.2518
Protective effects of Citrullus lanatus seed ethanol extract on aluminum chloride-induced testosterone, testicular and hematological changes in an experimental male rat model
Rita
Odo
Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Nigeria
author
Chukwuka
Uchendu
Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Nigeria
author
Somtoo
Okeke
Department of Veterinary Physiology and Pharmacology, University of Nigeria, Nsukka, Nigeria
author
text
article
2021
eng
The study was done to ascertain the protective potentials of ethanol seed extract of Citrullus lanatus on aluminum chloride-induced reproductive and hematological toxicities. Thirty mature male rats were used for the study. They were assigned into five groups (n=6). Group1was treated daily with aluminum chloride (100 mg kg-1) per os for 8 weeks. Group 2 was treated with aluminum chloride (100 mg kg-1) and C. lanatus seed extract (CLSE) 200 mg kg-1 per os simultaneously for 8 weeks. Group 3 was served as a normal control and given distilled water as a placebo per os daily for 8 weeks. Group 4 was only treated with CLSE (200mg kg-1) for eight weeks. Group 5 was only treated with aluminum chloride (100 mg kg-1) per os for 8 weeks and then treated with CLSE (200 mg kg-1) per os for another 4 weeks. Testosterone level, testicular weight, sperm motility, gonadal sperm, and extragonadal sperm reserves showed significant increases in group 2 compared to groups 1 and 5. Optimum histoarchitectural protection of the seminiferous tubules was observed in group 2, which did not differ from normal ones. For the hematological parameters, optimum protection was also observed in group 2 compared to other groups. From the results, ethanol seed extract of C. lanatus demonstrated protective potentials against aluminum's harmful effects on the male reproductive system and hematology in an experimental male rat model.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
7
13
https://vrf.iranjournals.ir/article_240933_6d468cf0badde267d7f0e21d421174ca.pdf
dx.doi.org/10.30466/vrf.2020.104327.2480
Molecular evidence and morphological aspects of Transversotrema licinum, Phyllodistomum hoggettae, and re-description of Gyliauchen volubilis (Digenea) from the Red Sea
Ali
Al-Ghamdi
Department of Biology, College of Science, Al Baha University, Al Baha, Saudi Arabia
author
Kareem
Morsy
Department of Biology, College of Science, King Khalid University, Abha, Saudi Arabia
author
Saad
Dajem
Department of Biology, College of Science, Al Baha University, Al Baha, Saudi Arabia
author
Ali
Shati
Department of Biology, College of Science, Al Baha University, Al Baha, Saudi Arabia
author
Mohammed
Al-Kahtani
Department of Biology, College of Science, Al Baha University, Al Baha, Saudi Arabia
author
Ahmed
Baiomy
Department of Zoology, Faculty of Science, Cairo University, Cairo, Egypt
author
Ahmed
Ezzat
Department of Biology, College of Science, Al Baha University, Al Baha, Saudi Arabia
author
text
article
2021
eng
During a recent survey on digenean parasites infecting marine fish captured from water located along the Red Sea off Gizan Coasts, Saudi Arabia, thirty-two (29.09%) out of 110 fish were naturally infected with three different species of digenean parasites. The taxonomical status of the recovered worms was confirmed by light microscopy and molecular analysis. Transversotrema licinuminfecting the haffara sea bream Rhabdosargus haffara possessed a transversely elongated body with eyespots and the anterior body margin with delineated velum along with its entire breadth. Oral sucker was absent, and eyespots were paired anterior to or at the level of ventral sucker. Phyllodistomum hoggettaefrom the urinary bladder of the Arabian Nagil Plectropomus areolatus was differentiated by its elongated body, narrow anterior end widened as going posteriorly with prominent marginal undulations in the hindbody. Gyliauchen volubilisinfecting the marbled spinefoot Siganus rivulatus, was with fleshy body, conical, tapered anteriorly, convex dorsally, and concave ventrally. Prepharynx was very long and convoluted and had occupied the greater part of the anterior half of the body. Multiple sequence alignment and phylogeny were based on maximum likelihood analysis for the ITS–2 and 28S rDNA for Transversotrema licinum and Phyllodistomum hoggettae, respectively, were constructed. They were compared to species within their families and further deposited into the Genebank under accession numbers MG 722711 and MG722710, respectively.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
15
24
https://vrf.iranjournals.ir/article_240921_17fe51c9861d14f057b90429e1d6144c.pdf
dx.doi.org/10.30466/vrf.2018.95586.2296
Fabrication of chitosan-based electrospun nanofiber scaffold: Amplification of biomechanical properties, structural stability, and seeded cell viability
Mohammad
Mahdian-Dehkordi
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Farshid
Sarrafzadeh-Rezaei
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mazdak
Razi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mehdi
Mahmoudian
Nanotechnology Research Center, Urmia University, Urmia, Iran
author
text
article
2021
eng
The cell scaffolds should structurally be manufactured similar to the target tissue's extracellular matrix. This property should be maintained until cell differentiation. For this purpose, in the current study, electrospun nanofiber (EN) of chitosan (Ch)/polyvinyl alcohol (PVA), as a tissue-friend scaffold, was fabricated by electrospinning in different formulations and borax was utilized as an innovative cross-linking agent to up-regulate the structural and biomechanical properties. The weight loss, water absorbability, structural stability, tensile strength and biocompatibility of borax-included and non-included ENs were compared. The finest morphology, weight loss, water absorbability, structural stability in an aqueous environment, tensile strength and cell viability were found in the borax-included EN containing Ch50.00%v/PVA50.00%v. Moreover, The ENs exhibited appropriate antibacterial properties against Gram-positive and Gram-negative bacteria. In conclusion, borax can be used to improve the mechanical and biocompatibility features of the Ch/PVA-based ENs. Furthermore, it could be suggested that borax-included Ch/PVA ENs can exhibit high appropriate biological properties, candidate them as an appropriate scaffold in the field of tissue engineering. However, in vivo trials are needed to clearly their side effects and advantages.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
25
32
https://vrf.iranjournals.ir/article_240939_09f5e7a8859091feb0ea4b5180882cc7.pdf
dx.doi.org/10.30466/vrf.2020.123047.2893
Comparison of artificial neural networks and multiple linear regression for prediction of dairy cow locomotion score
Mohammad Ali
Norouzian
Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Tehran, Iran
author
Hossein
Bayatani
Department of Animal and Poultry Sciences, College of Abouraihan, University of Tehran, Tehran, Iran
author
Mona
Vakili Alavijeh
Department of Soft Computing, Faculty of Mathematical Sciences, Shahid Beheshti University, Tehran, Iran
author
text
article
2021
eng
In this study, artificial neural networks (ANNs) were employed to investigate the relationship between locomotion score and production traits. A total number of 123 dairy cows from a free-stall housing farm were used in this study. To compare the effectiveness of the ANNs for the prediction of locomotion score, the multiple linear regression (MLR) model was developed using the eight production traits, body condition score, parity, days in milk, daily milk yield, milk fat percent, milk protein percent, daily milk fat yield, and daily milk protein yield as input variables to predict the locomotion score. The ANN predictions gave a higher coefficient of determination (R2) values with lower mean squared error (MSE) than MLR. The R2 and MSE of the MLR model were 0.53 and 0.36, respectively. However, the ANN model for the same dataset produced much improved results with R2 = 0.80 and MSE = 0.16, respectively. Globally, the results of this study showed that the connectionist network model was a better tool to predict locomotion scores compared to the multiple linear regression.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
33
37
https://vrf.iranjournals.ir/article_240940_62b45abf9f8ad1497116fb1fec168263.pdf
dx.doi.org/10.30466/vrf.2019.98275.2346
Differences between some biochemical components in seminal plasma of first and second ejaculations in dual-purpose Simmental (Fleckvieh) bulls and their relationships with semen quality parameters
Amir
Khaki
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
author
Atefeh
Araghi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran
author
Mehdi
Lotfi
Iran Simmental Cattle
Breeding Center, Amard-Dam Company, Amol, Iran
author
Alireza
Nourian
Iran Simmental Cattle
Breeding Center, Amard-Dam Company, Amol, Iran
author
text
article
2021
eng
This study aimed to evaluate differences in seminal plasma zinc (Zn), copper (Cu), iron (Fe), catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) levels in the first and second ejaculations and their relationships with semen quality parameters in Fleckvieh bulls. Repetitive ejaculates were separately collected, analyzed, and frozen from the sires. Progressive motility of frozen-thawed semen (PMFT) was considered the main factor for more data classification into three following groups: <40.00%, 40.00 - 50.00%, and >50.00%. Seminal plasma trace elements and enzymes were determined using atomic absorption spectrometry and ELISA, respectively. The results revealed significant differences between the first and second ejaculations. Semen concentration, SOD, GPx, and Fe were different in ejaculations. Although PMFT groups in different ejaculations did not show significant differences, there was significant alteration between different PMFT groups and first and second ejaculations. All frozen-thawed semen CASA parameters (except lateral head displacement) were associated with fresh motility parameters and before and after thawing sperm viability. Also, a correlation between seminal Zn concentration with fresh semen gross and progressive motility, average path velocity, and beat cross frequency, Cu with SOD and Fe and semen concentration was observed. CAT was associated with fresh and frozen-thawed sperm motility parameters except for lateral head displacement and angular displacement. Although our findings showed differences between the first and second ejaculations in some parameters, PMFT, which is the most important indicator for estimating bull fertility, was not different between them.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
39
46
https://vrf.iranjournals.ir/article_240928_3a6361e06f2b4ffbea5491c66286f643.pdf
dx.doi.org/10.30466/vrf.2019.98781.2355
Molecular effects of curcumin on the experimental autoimmune encephalomyelitis
Laleh
Mavaddatiyan
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
author
Shiva
Khezri
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
author
seyyed Meysam
Abtahi Froushani
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2021
eng
Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS). Previous studies have shown that myelin degradation during MS and EAE resulted in reduced expression of some of the proteins, e.g., the MBP (myelin basic protein), and increased expression of genes such as iNOS (Inducible nitric oxide synthase) and NOGO-A in the affected patients. In the present study, EAE was induced by immunizing Wistar rats (n=12) with homogenized spinal cord of guinea pig and Freund's complete adjuvant. Curcumin is an active ingredient in turmeric with anti-inflammatory properties, which has been studied in this article. In this study, the effect of curcumin administration on the change of the expression of MBP, NOGO-A, and iNOS genes was evaluated using the RT-PCR (Reverse transcription-polymerase chain reaction) technique. The obtained results indicated it could be concluded that curcumin was able to improve EAE by increasing the amount of MBP gene expression and reducing the intensity of NOGO-A expression.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
47
52
https://vrf.iranjournals.ir/article_240929_326d728b3cba23c212d3f3170a45f9c9.pdf
dx.doi.org/10.30466/vrf.2019.98789.2356
Effectiveness of nuchal ligament autograft in the healing of an experimental superficial digital flexor tendon defect in equid
Ahmad
Khajeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Ali
Baniadam
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Ahmad
Oryan
Department of Pathology,
School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Ali Reza
Ghadiri
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Hadi
Naddaf
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2021
eng
This study aimed to investigatenuchal ligament (NL) autograft on experimental tendon defect healing in donkeys. Eight healthy donkeys were used. The left forelimb’s superficial digital flexor (SDF) tendon was assigned as treatment, and the right forelimb was allocated as the control group (without surgical intervention). A 3×1.5 cm segment of the funicular part of the NL was excised. A full thickness defect created in the treatment tendon and was grafted with the excised NL. The following parameters were evaluated in 120 days postoperatively: clinical, ultrasonography, radiography, histopathology, biomechanical properties, and scanning and electronic transmission microscopy. There were no significant changes observed in the neck angle so that it was confirmed this treatment regimen preserved the head and neck situation without any considerable neck swelling. Weight-bearing in gait and trot was similar between both forelimbs at the end of the study. Mild to moderate adhesion was detected in the dorsal surface of the SDF tendon. There was no significant difference in the echogenicity and fiber alignment, respectively, on days 90 and 120 after surgery. Treatment significantly amplified the collagen diameter and enhanced the collagen fibril diameter and density considerably compared to the NL. The transplanted tissue was mostly in the remodeling or maturation phase, on day 120 postoperatively. It seems thatthe NL is biocompatible, almost biodegradable, and effective in tendon healing without metaplasia or tissue rejection.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
53
61
https://vrf.iranjournals.ir/article_240927_66133aaea9e1ec2284bf8afd381d75a1.pdf
dx.doi.org/10.30466/vrf.2019.97919.2330
Effect of dietary L-tryptophan supplementation and light-emitting diodes on growth and immune response of broilers
Hossein
Sharideh
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
Mojtaba
Zaghari
Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
author
text
article
2021
eng
Light-emitting diodes (LEDs) lights are more energy-efficient and provide adequate illumination compared to compact fluorescent (CFL) lamps and incandescent light (ICD) bulbs. However, as new light sources, the LED lights may have a stress effect on broiler chickens. Thus, this study aimed to determine the effects of dietary L-tryptophan (Trp), as an anti-stress agent and different color temperatures of light-emitting diodes on immune responses and growth performance of male broiler chickens. Four hundred and eighty day-old Ross 308 male chicks were used from day 1 to 42. The chicks were randomly distributed into six treatment groups in a 2 × 3 factorial arrangement [0 or 1 g Trp per kg diet along with neutral-white (4286 K), warm-white (2990 K), and incandescent (2790 K) light bulbs] with four replicates of 20 chicks each. Results showed that dietary Trp and Trp×light interaction did not affect growth performance, immune responses, a total number of leukocytes, and different leukocytes count (heterophil, eosinophil, monocyte, and lymphocyte) of male broiler chickens. However, LEDs’ different color temperatures significantly affected the feed conversion ratio (FCR) and primary antibody of sheep red blood cell (SRBC). The FCR was the lowest in the warm-white light, and primary SRBC antibody titers of the chicks were the highest. In conclusion, although adding Trp to male broiler diets did not affect the growth performance and immune responses of chickens, the warm-white light improved the FCR and primary SRBC.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
63
67
https://vrf.iranjournals.ir/article_240925_8c7341b2529d03fb312f6b387dafc181.pdf
dx.doi.org/10.30466/vrf.2019.96558.2310
Tenogenic effects of silymarin following experimental Achilles tendon transection in rats
Hazhir
Soleimani
DVM Graduate, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mehdi
Behfar
Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Rahim
Hobbenaghi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2021
eng
Tendon healing is prolonged due to the small number of cells, poor circulation, and low metabolism. The optimal tendon healing and its complete functional recovery have always been a challenge for researchers. Silymarin possesses anti-inflammatory, anti-oxidant, analgesic, and regenerative properties. The present study aimed to investigate the effects of silymarin on healing the Achilles tendon in rats. Twenty-four male Wistar rats were divided into two groups of control and treatment. After surgical preparation, a complete transverse incision was made in the middle part of the Achilles tendon, and then a modified Kessler suture was placed. The control group received 1.00 mL normal saline for five consecutive days, and the treatment group received 50.00 mg kg-1 of silymarin suspended in 1.00 mL normal saline for five days, orally. During the experimental period, Achilles functional index (AFI) was recorded. Six weeks after surgery, sampling was done. Histopathologically, a significant increase in the density of collagen fibers and reduction in neovascularization and inflammatory cells infiltration were observed in the treatment group. The biomechanical evaluation showed a significant increase in tensile strength of the tendon in the treatment group compared to the control group. The AFI results were concomitant with the results stated above, indicating an improvement in the AFI of rats in the treatment group. The present study results showed that oral administration of silymarin improved tissue healing indices, biomechanical properties, and functional index, leading to optimal healing of experimental Achilles tendon injury in the rat.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
69
75
https://vrf.iranjournals.ir/article_240936_17be1e390f6dd8ea1670d1cf0a50dcaf.pdf
dx.doi.org/10.30466/vrf.2019.112403.2678
Caffeic acid protects mice pancreatic islets from oxidative stress induced by multi-walled carbon nanotubes (MWCNTs)
Akram
Ahangarpour
Diabetes Research Center, Health Research Institute, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz,
Iran
author
Soheila
Alboghobeish
Student Research Committee, Department of Pharmacology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
Ali Akbar
Oroojan
Student Research Committee, Department of Physiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Science, Ahvaz, Iran
author
Mohammad
Dehghani
Student Research Committee, Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
author
text
article
2021
eng
Increasing applications of carbon nanotubes (CNTs) indicate the necessity to examine their toxicity. According to previous studies, CNTs caused oxidative stress that impaired β-cell functions and reduced insulin secretion. Our previous study indicated that single-walled carbon nanotubes (SWCNTs) could induce oxidative stress in pancreatic islets. However, there is no study on the effects of multi-walled carbon nanotubes (MWCNTs) on islets and β-cells. Therefore, the present study aims to evaluate effects of MWCNTs on the oxidative stress of islets and the protective effects of caffeic acid (CA) as an antioxidant. The effects of MWCNTs and CA on islets were investigated using MTT assay, reactive oxygen species (ROS), malondialdehyde (MDA), activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), the content of glutathione (GSH) and mitochondrial membrane potential (MMP) and insulin secretion measurements. The lower viability of islet cells was dose-dependent due to the exposure to MWCNTs according to the MTT assay. Further studies revealed that MWCNTs decreased insulin secretion and MMP, induced ROS creation, increased the MDA level, and decreased activities of SOD, GSH-Px, CAT, and content of GSH. Furthermore, the pretreatment of islets with CA returned the changes. These findings indicated that MWCNTs might induce the oxidative stress of pancreatic islets occurring diabetes and protective CA effects that were mediated by the augmentation of the antioxidant defense system of islets. Our research suggested the necessity of conducting further studies on effects of MWCNTs and CA on the diabetes.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
77
85
https://vrf.iranjournals.ir/article_240923_ebd137cbf5cc81db33618e3ec658a877.pdf
dx.doi.org/10.30466/vrf.2019.94666.2279
Computational study of zebrafish immune-targeted microarray data for prediction of preventive drug candidates
Abdolvahab
Ebrahimpour Gorji
Department of Fisheries, Faculty of Animal Sciences and Fisheries, Sari Agricultural and Natural Resources University, Sari, Iran
author
Zahra
Roudbari
Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran
author
Fatemeh
Ebrahimpour Gorji
Department of Cell and Molecular Biology, Faculty of Science, University of Andishesazan, Neka, Iran
author
Balal
Sadeghi
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2021
eng
Viral hemorrhagic septicemia virus (VHSV) is a rhabdovirus reported to cause economic loss in fish farms. Because of the lack of adequate preventative treatments, the identification of multipath genes involved in VHS infection might be an alternative to explore the possibility of using drugs for the seasonal prevention of this fish disease. We propose labeling a category of drug molecules by further classification and interpretation of the Drug Gene Interaction Database using gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment scores. The study investigated disease networks of up-and down-regulated genes to find those with high interaction as substantial genes in pathways among the different disease networks. We prioritized these genes based on their relationship to those associated with VHS infection in the context of human protein-protein interaction networks and disease pathways. Among the 29 genes as potential drug targets, nine were selected as promising druggable genes (ERBB2, FGFR3, ITGA2B, MAP2K1, NGF, NTRK1, PDGFRA, SCN2B, and SERPINC1). PDGFRA is the most important druggable up-and down-regulated gene and is considered an important gene in the IMATINIB pathway. This study findings indicate a promising approach for drug target prediction for VHS treatment, which might be useful for disease therapeutics.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
87
93
https://vrf.iranjournals.ir/article_240920_f3f5ea0db66bf7be1780c9b0eaca0951.pdf
dx.doi.org/10.30466/vrf.2019.94179.2270
In vitro assessment of anti-Trichomonas effects of Zingiber officinale and Lavandula angustifolia alcoholic extracts on Trichomonas gallinae
Farnaz
Malekifard
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mosa
Tavassoli
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mohammad
Alimoradi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2021
eng
Trichomonas gallinae is a parasite that acts as a canker-causing agent and leads to significant loss and mortality, especially in young birds. Metronidazole is the approved drug used for the treatment of trichomoniasis. A non-chemical alternativess such as medical plant extracts are also used to treat this disease due to drug resistance. This study aimed to assess in vitro antitrichomonal effects of Lavandula angustifolia and Zingiber officinale extracts on T. gallinae compared with metronidazole. The T. gallinae samples were obtained from infected pigeons. Multi-well plates filled with different concentrations (5.00, 10.00, 25.00, 50.00, and 100 μg mL-1) were used to perform in vitro analysis. The Z. officinaleextract’s minimum inhibitory concentration (MIC) in the 24-hr period was 25.00 μg mL-1, while it was 50.00 μg mL-1 for metronidazole. The MIC value obtained for L. angustifolia extract in 24-hr was 50.00 μg mL-1. The results indicated that the extracts of Z. officinaleand L. angustifolia could act as potential natural agents against trichomoniasis. Furthermore, this study delineated the equal efficiency of L. angustifolia and Z. officinale with that of metronidazole in inhibiting the growth of Trichomonas gallinae trophozoites in culture media.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
95
100
https://vrf.iranjournals.ir/article_240931_14d208a1249b7be8d8743a87e14ea3a5.pdf
dx.doi.org/10.30466/vrf.2019.102620.2444
High incidence of multidrug resistance and class 1 and 2 integrons in Escherichia coli isolated from broiler chickens in South of Iran
Mohsen
Kalantari
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Hassan
Sharifiyazdi
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Keramat
Asasi
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Bahman
Abdi-Hachesoo
Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
text
article
2021
eng
The objective was to investigate the multidrug resistance and presence of class 1 and 2 integrons in 300 Escherichia coli isolates obtained from 20 broiler farms during three rearing periods (one-day-old chicks, thirty-day-old chickens, and one day before slaughter) in Fars, South Iran. Results showed that 81.00%, 82.00%, and 85.00% of isolates were multidrug-resistant on the first day, thirty-day-old chickens, and one day before slaughter, respectively. Multidrug-resistant E. coli isolates were further examined for the presence of class 1 and 2 integrons using PCR assay. The existence of class 1 integron-integrase gene (intI1) was confirmed in 68.40%, 72.70%, and 60.90% of multidrug-resistant isolates from stage 1, stage 2, and stage 3 of the rearing period, respectively. The frequency of class 2 integron-integrase gene (intI2) during the first to the third stage of sampling was 2.60%, 25.50%, and 30.40%. Also, sequence analysis of the cassette arrays within class 1 integron revealed the presence of the genes associated with resistance for trimethoprim (dfrA), streptomycin (aadA), erythromycin (ereA), and orfF genes. The results revealed that percentages of antimicrobial resistance in E. coli isolates were significantly higher in the middle and end stages of the rearing period. In conclusion, widespread dissemination of class 1 integrons in all three stages and rising trends of class 2 integrons existence in E. coli isolates during the rearing period of broiler chickens could exacerbate the spread of resistance factors among bacteria in the poultry industry. Future research is needed to clarify its implication for human health.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
101
107
https://vrf.iranjournals.ir/article_240922_dda7631ac711fd93539dc84746677810.pdf
dx.doi.org/10.30466/vrf.2019.96366.2309
Effects of liraglutide on sperm characteristics and fertilization potential following experimentally induced diabetes in mice
Maryam
Pourheydar
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Shapour
Hassanzadeh
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mazdak
Razi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Bagher
Pourheydar
Department of Anatomical Sciences, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran
author
Gholamreza
Najafi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2021
eng
The current study was conducted to analyze the dose-dependent effects of liraglutide against the diabetes-induced detrimental impact on sperm parameters and fertilization potential. For this purpose, 42 adult male mice were randomly divided into control (with no intervention) and experimental groups. Next, the experimental group was subdivided into diabetic, 1.20 mg kg-1 liraglutide-received diabetic, 1.80 mg kg-1 liraglutide-received diabetic, 1.20 mg kg-1 liraglutide-received non-diabetic and 1.80 mg kg-1 liraglutide-received non-diabetic groups. All chemicals were administrated subcutaneously. Following 42 days, the animals were euthanized, and sperm samples were collected. The sperm count, motility, viability, DNA integrity, and maturity were analyzed and compared between groups. Moreover, the sperm fertilization potential was investigated by in vitro fertilization (IVF). For this purpose, the preimplantation embryo development at 2-cell, 4-cell, morula, and blastocyst stages was investigated and compared. Observations revealed that diabetes significantly diminished sperm count, motility, viability, chromatin condensation, and DNA integrity percentages versus a control group. On the other hand, 1.20 mg kg-1 and 1.80 mg kg-1 of liraglutide did not improve sperm motility and viability, while ameliorated sperm count and chromatin condensation and DNA integrity in diabetic animals. The diabetic animals represented diminished preimplantation embryo development, which was not altered in liraglutide-received groups. In conclusion, at least in administrated doses, liraglutide could not improve the sperm viability and motility and, via this mechanism, could not induce an appropriate/beneficial effect on IVF outcome.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
109
116
https://vrf.iranjournals.ir/article_240926_8765d64f9b4cbd59d29f27ea8b3bc223.pdf
dx.doi.org/10.30466/vrf.2019.96822.2315
Detection and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks in Sulaimani Governorate
Hana
Raoof
Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq
author
Mohammed
Baba Shiekh
Veterinary Laboratory, PCR Unit, Directorate of Veterinary, Sulaimani, Sulaymaniyah, Iraq
author
Rizgar
Sulaiman
Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq
author
text
article
2021
eng
Infectious Bronchitis (IB) is one of the most important viral diseases of poultry. It causes major financial losses in the poultry industry. It is caused by the infectious bronchitis virus (IBV). This study investigated the genetic origin and diversity of IBV by analyzing the partial S1 sequence derived from local broiler farms in different regions of Sulaimani Governorate. Detection was performed using a newly designed primer for the partial S1 gene. The present was undertaken to understand the molecular relationship between the strains across the world compared with field virus isolates by phylogenetic tree and sequences-analysis. Phylogenetic tree demonstrated that field isolate made a novel group (sul-5/17-like strain), indicating new variants. Also, field virus sequences were
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
117
120
https://vrf.iranjournals.ir/article_240924_0637d2bd6b7ad4145c6a177b079735bb.pdf
dx.doi.org/10.30466/vrf.2018.94774.2281
Molecular identification of Uronema marinum (Protozoa, Ciliophora, Scuticociliatia) in cultured turbot (Psetta maxima) larvae
Mustafa
Türe
Department of Fisheries Health, Central Fisheries Research Institute, Trabzon, Turkey
author
text
article
2021
eng
Scuticociliates are dangerous parasitic pathogens causing systemic tissue destruction and high mortality in marine fish worldwide. In this study, the first identification of Uronema marinum (Ciliophora, Scuticociliatida) from cultured turbot (Psetta maxima) larvae using mitochondrial cytochrome c oxidase 1 (cox1) gene sequence as well as species-specific primers was reported. The mean prevalence values of infected fish were calculated, and partial sequencing obtained from the mitochondrial cox1 gene region was also compared with isolates registered in the Genbank database. The sequence comparison showed 93.00% identity to U. marinum, and the parasite has also been deposited in the GenBank database. This study is the first case of U. marinum infection in Turkish marine aquaculture, contributing to the systematics and molecular epidemiology of scuticociliate in Turkey.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
121
124
https://vrf.iranjournals.ir/article_240935_0dd2ce02855ff59aef134a7dcc581b3d.pdf
dx.doi.org/10.30466/vrf.2020.110220.2614
Estrogenic response in Japanese turtle (Mauremys japonica) exposed to petroleum hydrocarbon
Maurilio
Lara-Flores
Epomex Institute, Campeche University, Campeche, Mexico
author
Jaime
Rendon von Osten
Epomex Institute, Campeche University, Campeche, Mexico
author
text
article
2021
eng
Expressions of the estrogen receptors and vitellogenin in Japanese turtle (Mauremys japonica) in response to petroleum hydrocarbon were studied. A total of 15 male turtles were exposed to 1.00 mg L-1 of a sample of an oil spill, and 15 male and 15 female turtles were served as controls without an oil spill. The transcripts' results demonstrated an increase over time with greater expression of vitellogenin I in males exposed to petroleum with significant differences. In the case of vitellogenin II, the expression was greater than control males, but it was similar to the values of control females. Concerning the estrogen receptor a and estrogen receptor b, males exposed to oil spill presented higher values at 72 hr than the controls. In conclusion, in the present work, the effect of petroleum as an endocrine disruptor in turtles was demonstrated, and it can be used to identify damages induced by the presence of hydrocarbons in aquatic environments.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
125
128
https://vrf.iranjournals.ir/article_240932_f5d38b93655a6af0f6aea2561fa0cf94.pdf
dx.doi.org/10.30466/vrf.2019.103856.2466
Occurrence of myxoma in a bearded dragon (Pogona vitticeps): Surgical and histopathological studies
Sara
Shokrpoor
Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Mirsepehr
Pedram
Department of Radiology and Surgery, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Niyousha
Torjani
DVM Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Reza
Khoshvaghti
DVM Student, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2021
eng
Myxoma is a benign mesenchymal tumor of skin and soft connective tissues considered to be of fibroblastic origin. A female bearded dragon (Pogona vitticeps) with a raised mass on the left side of the body was referred to the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.The mass had become evident and grew more extensive within the previous 11-month period. The dragon was anesthetized with 5.00% isoflurane, and the mass was removed surgically. Tissue samples of the mass were fixed in 10.00% neutral buffered formalin, sectioned and stained with Hematoxylin and Eosin (H&E), Alcian blue (pH: 2.50), and periodic acid Schiff (PAS) staining techniques. The patient was administered meloxicam, lincomycin, and marbofloxacin. Histopathologically, the capsulated mass was composed of myxoid structure. Stellate-to-reticular cells distributed in a mucinous stroma containing few tiny blood vessels were seen. Myxoid cells displayed a PAS-positive cytoplasm. The myxomatous matrix was alcianophilic at the pH of 2.50. To our knowledge, this is the first report of surgery, treatment, and histopathological study of myxoma in a bearded dragon (P. vitticeps). The sutures were also removed 12 days after the operation. No new growth of the mass was observed three months following the surgical procedures.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
129
131
https://vrf.iranjournals.ir/article_240938_918bdebd85e462302478a4f5a2db2e8c.pdf
dx.doi.org/10.30466/vrf.2020.119465.2824
Clinical report of a Holstein's calf with ichthyosis
Sina
Moghaddam
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Amir Farhang
Houshangi
Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
author
Behrad
Eshratkhah
Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
author
Rahman
Allahvirdizadeh
Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2021
eng
Congenital ichthyosis is a disease associated with hair loss and the presence of horny plates in the epidermis, covering the whole skin. The leading cause of the disease in humans and animals is genetic disorders, and they can be found in mild and severe forms. In June 2015, a newly born calf of the Holstein breed was referred to the Livestock Veterinary Hospital internal ward, Shabestar, Iran. The calf's clinical symptoms included maternal alopecia, thickening, and fissure over large areas of the body, tough skin with thick horny scales and deep crack, lack of flexibility in the body parts (gluteal, knee, and shoulder areas), ectropion, eclabium, and microtia. The blood samples were taken from the calf's jugular vein to measure the hematological and biochemical parameters. After euthanizing the calf, the skin of different body regions was sampled for histopathological examination of skin lesions. Based on the results, the amounts of plasma parameters such as urea, triglyceride, glucose, alanine transaminase, lactate dehydrogenase, phosphorus, and uric acid were increased. The leukocytosis and polycythemia were found in the hematology results, and histopathological analysis exhibited hypergranulosis and hyperkeratosis in the skin of affected areas. Ichthyosis is caused by the defect in the autosomal recessive gene and as an incurable disease; there is currently no cure for this deadly disease, and the livestock will be eliminated from the herd.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
12
v.
1
no.
2021
133
135
https://vrf.iranjournals.ir/article_240937_4e228df44a02379a3584b476b6d26b28.pdf
dx.doi.org/10.30466/vrf.2020.117113.2783