Auto-transplantation of whole rat ovary in different transplantation sites
Somayeh
Tavana
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
author
Mojtaba
Rezazadeh Valojerdi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
author
Hussein
Eimani
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
author
Naeimeh Sadat
Abtahi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
author
Rouhollah
Fathi
Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
author
text
article
2017
eng
This study was carried out to assess the different ovarian transplantation sites after short-time autografting. Female rats were randomized into five groups, with six rats in each group, including control (intact), cervical subcutaneous transplanted (CST), back subcutaneous transplanted (BST), subfascial transplanted (SFT) and intramuscular transplanted (IMT) groups. In all experimental groups, the right ovary was removed and transplanted into different sites. After three weeks, ovaries were removed for morphology assessment, follicular counting and the rates of corpus luteum (CL) and cyst formation. Transplanted ovaries in BST and SFT groups were full of cysts and did not have sufficient numbers of intact follicles and were excluded from experiments. In IMT and CST groups, re-anastomosis, follicular development and good homogeneity of the stromal tissue were seen. However, the difference in intact antral follicles between CST (7.92 ± 0.02%) and CST-Op (opposite ovary of CST group) (30.99 ± 0.03%) was significant as well as the difference between CST (7.92 ± 0.02%) and control (10.08 ± 0.01%) groups. In addition, the number of intact primordial follicles in the CST-Op (16.58 ± 0.02%) group was significantly less than that of the control (40.40 ± 0.03%) group. Interestingly, the number of CL was significantly increased in the CST-Op (11.71 ± 0.01%) and IMT-Op (9.16 ± 0.02%) groups compared to the control and experimental groups. Although both intramuscular and subcutaneous sites effectively preserved ovarian follicles after three weeks, cervical subcutaneous site was better suited for auto-transplantation in rat.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
275
280
https://vrf.iranjournals.ir/article_28485_0434a38ea31fa10a40bfb0e86be3af29.pdf
Effect of Satureja khuzestanica essential oil against fertility disorders induced by busulfan in female mice
Abbas
Ahmadi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Saleh
Bamohabat Chafjiri
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Rajab Ali
Sadrkhanlou
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2017
eng
Busulfan is an alkylating agent affects ovarian follicles growth by oxidative stress induction. Satureja khuzestanica has antioxidant effects. The aim of this study was to examine whether S. khuzestanica essential oil (SKEO) exhibits protective effects on busulfan-induced ovarian failure. Eighty-four adult female mice were divided into six groups including dimethyl sulfoxide (control), SKEO 225.00 mg kg-1 (orally), busulfan 3.00 mg kg-1 (orally), busulfan 36.00 mg kg-1 (intraperitoneally), busulfan 3.00 mg kg-1 and SKEO and busulfan 36.00 mg kg-1 and SKEO. After 28 days, the mice were euthanized and oocytes were removed for in vitro fertilization (IVF) rate evaluation. Oocyte quantity and quality, fertilization rate and pre-implantation embryo development were daily examined with a stereo microscope in a period of 120 hr. Serum levels of estradiol and progesterone were also evaluated. Busulfan caused significant decreases in oocyte number and quality, fertilization rate, pre-implantation embryo development and embryo quality. The SKEO significantly decreased the adverse effects of busulfan. The present study indicated that SKEO can protect female fertility potential against busulfan induced damages.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
281
286
https://vrf.iranjournals.ir/article_28477_5b39341018a1b705105e8bee944b605f.pdf
Molecular identification and phylogenetic analysis of chronic bee paralysis virus in Iran
Mohammadreza
Ghorani
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Arash
Ghalyanchi Langeroudi
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Omid
Madadgar
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Mohammadreza
Rezapanah
Center of Excellence for Organic Agriculture and Department of Biological Control, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
author
Sedigheh
Nabian
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
Reza
Khaltabadi Farahani
Iranian Veterinary Organization, Tehran, Iran
author
Hossein
Maghsoudloo
Iranian Veterinary Organization, Tehran, Iran
author
Mohammad
Forsi
Iranian Veterinary Organization, Tehran, Iran
author
Hamed
Abdollahi
Iranian Veterinary Organization, Tehran, Iran
author
Hesameddin
Akbarein
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
author
text
article
2017
eng
Chronic bee paralysis virus (CBPV) is an unclassified polymorphic single-stranded RNA virus. Among the viruses infecting honeybees, CBPV is known to induce significant losses in honeybee colonies. In this study, a total number of eighty-nine suspected apiaries from four regions of Iran (including Mazandaran, Khorasan Razavi, Hormozgan, and Kurdistan) were sampled and submitted for molecular identification. Three positive samples were detected by RT-PCR. All positive samples were confirmed by sequencing. The phylogenetic tree which displays the molecular relationship between the viruses of different Iranian geographic regions and references isolates was constructed. The Iranian isolates formed two distinct phylogenetic groups (Group 1 and Group 2). The IR-CPV-GMG-1, IR-CPV-GMG-2, IR-CPV-GMG-4, and IR-CPV-GMG-6 formed Group 1 and IR-CPV-GMG-3, IR-CPV-GMG-5, and IR-CPV-GMG-7 were in Group 2 as a distinct group. Iranian isolates in group 1 were similar to European and East Asian CBPVs. This research was the first phylogenetic analysis of CBPV in Iran. Further researches are needed to study the other aspects of this virus-like genetic characteristics and pathogenesis in Iran.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
287
292
https://vrf.iranjournals.ir/article_28478_08a2cc92f8b06bf6f6e2b033a4e9f1a8.pdf
Molecular detection and phylogenetic analysis of Mycoplasma gallisepticum from backyard and commercial turkey flocks in Iran
Saeed
Rasoulinezhad
Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Mohammad Hassan
Bozorgmehrifard
Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Hossein
Hosseini
Department of Clinical Science, Faculty of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
author
Nariman
Sheikhi
Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
Saeed
Charkhkar
Department of Poultry and Obstetrics, Science and Research Branch, Islamic Azad University, Tehran, Iran
author
text
article
2017
eng
Mycoplasma gallisepticum (MG) is economically important pathogen of poultry causes airsacculitis and frequently infraorbital sinusitis in turkeys. Infections may remain without clinical signs, but they can make birds susceptible to secondary infections.This study was carried out for molecular detection and phylogenetic analysis of MG infections in commercial and backyard turkey flocks in some parts of Iran. A total number of 600 swab samples were collected from 18 commercial and 31 backyard turkey flocks. The PCR technique was performed for detecting 16S rRNA gene in the samples. Positive sample were subjected for sequencing of mgc2 gene. The results showed that 48.38% of backyard and 16.66% of commercial farms were positive for MG. These findings suggested the presence of MG in the commercial and backyard turkeys’ farms of Iran. The molecular analysis indicated high sequence similarity between some Iranian turkeys isolates with Indian and Pakistanian MG isolates. Furthermore, substitutions of MG nucleic acids and correlated amino acids sequences may lead to some antigenic modifications.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
293
298
https://vrf.iranjournals.ir/article_28486_fa442463cbbe94a6080d3d7e2421e695.pdf
A molecular study on Babesia spp. in cattle and ticks in West-Azerbaijan province, Iran
Sepideh
Rajabi
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Bijan
Esmaeilnejad
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Mousa
Tavassoli
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2017
eng
A total number of 450 blood samples were collected from 45 different randomly selected cattle herds. Light microscopic examination of blood smears revealed Babesia spp. infection in 4.2%, while 8.9% of blood samples were positive using PCR. Upon multiplex-PCR (mPCR), B. bigemina and B. bovis infections were detected in 37/40 (92.5%) and 3/40 (7.5%) samples, respectively. 530 ticks of 10 Ixodid species were collected from the same cattle. Hyalomma anatolicum was the most prevalent tick species (19.9%). An expected 520 bp fragment of Babesia spp. was generated in 22 (48.8%) of Rhpicephalus annulatus, 18 (40.0%) of R. bursa and 12 (30.0%) R. sanguineus sensu lato. The mPCR findings revealed that all infected ticks including R. annulatus, R. bursa and R. sanguineus were totally infected with B. bigemina. The DNA amplification of B. bovis and B. bigemina in egg samples showed that only B. bigemina was detected in two specimens of R. annulatus. It could be concluded that B. bigemina was the dominant causative agent in this region but the evidence of B. bovis infection of cattle in a few cases was noted, as well. The results suggested that B. bigemina and B. bovis could be detected in the DNA extracted from R. annulatus, R. bursa and R. sanguineus sensu lato confirming previous reports. Since B. bigemina is transmitted transovarially by R. annulatus, it might act as an important vector for B. bigemina.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
299
306
https://vrf.iranjournals.ir/article_28480_9ea36f1694396b990b217b0767337fd0.pdf
Production and purification of polyclonal antibody against F(ab')2 fragment of human immunoglobulin G
Hadi
Nasiri
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Zahra
Valedkarimi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Leili
Aghebati-Maleki
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Jalal
Abdolalizadeh
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Tohid
Kazemi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Mojghan
Esparvarinha
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
Jafar
Majidi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
author
text
article
2017
eng
Antibodies are essential tools of biomedical and biochemical researches. Polyclonal antibodies are produced against different epitopes of antigens. Purified F(ab')2 can be used for animal’s immunization to produce polyclonal antibodies. Human immunoglobulin G (IgG) was purified by ion exchange chromatography method. In all stages verification method of the purified antibodies was sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Purified IgG was digested by pepsin enzyme and F(ab')2 fragment was purified by gel filtration separation method. For production of polyclonal antibody, rabbit was immunized by purified F(ab')2 and antibody production was investigated by enzyme-linked immunosorbent assay. Purified anti-IgG F(ab')2 was conjugated with fluorescein isothiocyanate. Ion exchange chromatography purification yielded 38 mg of human IgG antibody. The results of SDS-PAGE in reduced and non-reduced conditions showed bands with 25-30 kDa molecular weight (MW) and 50-kDa respectively and a distinct band with 150 kDa MW. The results of non-reduced SDS-PAGE for determining the purity of F(ab')2 fragment showed one band in 90 kDa and a band in 150 kDa MW position. Purification by Ion exchange chromatography method resulted about 12 mg rabbit polyclonal antibody. Flow cytometry showed generated polyclonal antibody had an acceptable activity compared to commercial antibody. Taking together, purified IgG F(ab')2 and polyclonal anti-IgG F(ab')2 are useful tools in biomedical and biochemical researches and diagnostic kits.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
307
312
https://vrf.iranjournals.ir/article_28908_2540034cec973212f75a104813cc8c5c.pdf
Microbiological, biochemical and organoleptic properties of fermented-probiotic drink produced from camel milk
Saideh
Saljooghi
Graduated Student, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
Ladan
Mansouri-Najand
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
Hadi
Ebrahimnejad
Department of Food Hygiene and Public Health, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
Farideh
Doostan
Department of Nutrition, Faculty of Health, Kerman University of Medical Sciences, Kerman, Iran
author
Nasrin
Askari
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2017
eng
The microbiological and biochemical changes occurred during the fermentation of camel milk inoculated by three selected bacterial starter, were investigated as well as the sensory evaluation of the product. Milk samples were collected from camel herds of southeastern of Iran. Chr. Hansen ABT-10 starter including Lactobacillus acidophillus, Biphidobacterum biphidum and Sterptococcus thermophilusin ratio of 0.50 g per 100 mL of camel milk was added. This fermented product was examined at the 0, 3rd, 6th and 9th days for microbiological, biochemical and sensory evaluations. The results showed the number of starter bacteria was maintained at least 106 CFU mL-1 during nine test days. It was shown that it could be used as fermented-probiotic drink. The product did not show any microbial contamination. The acidity and protein amount of produced drink showed a significant (p < 0.05) increase in different test days. Fat, solids-not-fat and ash amount of the product showed significant differences at the ninths’ test day compared to the zero test day (p < 0.05). Organoleptic properties of product including flavor, color, odor, consistency, mouth feel and overall acceptance were significantly improved (p < 0.05). Therefore, the produced fermented–probiotic drink, in addition to keep maintenance and increased nutritional quantity value, was accepted by consumers in terms of organoleptic properties and it could be used as a healthy and functional drink.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
313
317
https://vrf.iranjournals.ir/article_28482_3b5d480145d02e22724833e9caf24a04.pdf
Effect of chlorpyrifos on sperm characteristics and testicular tissue changes in adult male rats
Mojtaba
Babazadeh
Graduate Student, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Gholamreza
Najafi
Department of Basic Sciences, Faculty of Veterinary Medicine,
Urmia University, Urmia, Iran
author
text
article
2017
eng
Chlorpyrifos (CPF) is a broad spectrum organophosphate pesticide used for agricultural health purposes. Its principal mechanism of toxicity is the inhibition of acetylcholinesterase. The purpose of present study was to investigate the effects of CPF on testicular tissue and sperm parameters in male rats. Thirty-two healthy male rats were divided into two groups: a CPF-exposed group and a control-sham group. Control-sham group received corn oil (0.20 mL per day). The CPF was administered orally to male rats at 37 mg kg-1 BW for 45 days to evaluate the reproductive toxicity. In all rats, sampling for histological and sperm analyses was performed on days 5, 15, 30 and 45. The CPF caused a significant (p < 0.05) decrease in sperm count, viability and motility and increased immature sperms and DNA damage in sperm cells. Light microscopic analyses revealed increased arrested spermatogenesis, negative tubular differentiation and repopulation indexes and decreased Leydig cells number. These findings indicate that CPF has adverse effects on sperm cells and reproductive system of male rats.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
319
326
https://vrf.iranjournals.ir/article_28479_ae3a192b72651d8a318d3548cec55f3f.pdf
Florfenicol pharmacokinetics following intravenous and oral administrations and its elimination after oral and bath administrations in common carp (Cyprinus carpio)
Abdolhossein
Jangaran Nejad
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Rahim
Peyghan
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Hossein
Najafzadeh Varzi
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
Ali
Shahriyari
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
author
text
article
2017
eng
The aim of this study was to evaluate pharmacokinetic profiles of florfenicol after a single dose of intravenous (5.00 mg kg-1 body weight) and oral (40.00 mg kg-1 body weight) administrations in common carp (Cyprinus carpio). The residue depletion of florfenicol was also investigated after oral administration (10.00 mg kg-1 body weight) and bath treatment (5.00 mg L-1) for 10 consecutive days. Pharmacokinetics of florfenicol in plasma after a single dose administration, at 10 time points (0.50, 1, 2, 4, 8, 12, 24, 72, 120 and 168 hr) and florfenicol concentrations in tissues (plasma, liver and muscle) at three time points (1, 7 and 14 days) after 10 consecutive days, were analyzed by high performance liquid chromatography. The peak concentration of florfenicol was 137.02 ng mL-1 and the time to reach peak concentration in plasma was two hr. The elimination half-lives, the volume of distribution at steady state and total body clearance were estimated as 21.40 hr, 0.30 and 0.03 L hr-1, respectively. After drug administration for 10 days, it's concentration in plasma and muscle in oral treatment was significantly more than bath treatment in all days. Drug concentrations in the liver after bath treatment were significantly higher for a shorter period than the concentration in the oral treatment, indicating that higher levels of florfenicol for a longer period can be achieved in the tissues after oral drug administration. According to pharmacokinetic results, florfenicol may be a suitable candidate for the treatment of common bacterial infections in common carp farming. The aim of this study was to evaluate pharmacokinetic profiles of florfenicol after a single dose of intravenous (5.00 mg kg-1 body weight) and oral (40.00 mg kg-1 body weight) administrations in common carp (Cyprinus carpio). The residue depletion of florfenicol was also investigated after oral administration (10.00 mg kg-1 body weight) and bath treatment (5.00 mg L-1) for 10 consecutive days. Pharmacokinetics of florfenicol in plasma after a single dose administration, at 10 time points (0.50, 1, 2, 4, 8, 12, 24, 72, 120 and 168 hr) and florfenicol concentrations in tissues (plasma, liver and muscle) at three time points (1, 7 and 14 days) after 10 consecutive days, were analyzed by high performance liquid chromatography. The peak concentration of florfenicol was 137.02 ng mL-1 and the time to reach peak concentration in plasma was two hr. The elimination half-lives, the volume of distribution at steady state and total body clearance were estimated as 21.40 hr, 0.30 and 0.03 L hr-1, respectively. After drug administration for 10 days, it's concentration in plasma and muscle in oral treatment was significantly more than bath treatment in all days. Drug concentrations in the liver after bath treatment were significantly higher for a shorter period than the concentration in the oral treatment, indicating that higher levels of florfenicol for a longer period can be achieved in the tissues after oral drug administration. According to pharmacokinetic results, florfenicol may be a suitable candidate for the treatment of common bacterial infections in common carp farming.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
327
331
https://vrf.iranjournals.ir/article_28929_2728044afae52c17c39f9ca59f7ec9c0.pdf
European pond turtle (Emys orbicularis persica) as a biomarker of environmental pollution in Golestan and Mazandaran provinces, Iran
Somayeh
Namroodi
Department of Environmental Science, Faculty of Environment and Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
author
Annalisa
Zaccaroni
Department of Veterinary Public Health and Animal Pathology, Faculty of Veterinary Medicine, University of Bologna, Bologna, Italy
author
Hassan
Rezaei
Department of Environmental Science, Faculty of Environment and Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
author
Seyyedeh Malihe
Hosseini
Msc student, Department of Environmental Science, Faculty of Environment and Fisheries, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
author
text
article
2017
eng
Anthropogenic environmental changes are hypothesized as main reasons for animal species population declines. Heavy metals contamination is one of the worst threats to animals among human-caused threats. As most of the heavy metals bioaccumulate in organisms, analyzing concentrations of heavy metals in long living animals, such as turtles, would be very useful for biomonitoring of environmental quality. The European pond turtle is classified as a Near Threatened in the red list of International Union for Conservation of Nature. The objective of this study was to obtain information on heavy metals contamination in this species, as a sentinels, to evaluate the overall health of both the European pond turtles and their ecosystem in Golestan and Mazandaran provinces. Biological samples of 10 living and 15 dead European pond turtles were analyzed by atomic absorption spectrophotometer for Zn, Pb, Cu, and Cd contaminations. Highest concentration of Zn (202.6 ± 58.5 μg g-1), Cd (4.4 ± 1.3 μg g-1) and Cu (3.8 ± 1.7 μg g-1) was detected in livers and the highest accumulation of Pb (45.6 ±16.3 μg g-1) occurred in kidneys. Positive correlations were detected among Zn, Pb and Cd tissue concentrations and carapaces curve length. Heavy metal levels were higher in males than females. Heavy metals contamination of sampled turtles stood in high degree. However, there is clearly a need to evaluate heavy metals physiologic effects on European pond turtles.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
333
339
https://vrf.iranjournals.ir/article_28481_89c46a1c1f49c655fada819a201e171f.pdf
Effects of bisphenol-S low concentrations on oxidative stress status and in vitro fertilization potential in mature female mice
Alireza
Nourian
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Ali
Soleimanzadeh
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Ali
Shalizar Jalali
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
Gholamreza
Najafi
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
author
text
article
2017
eng
Bisphenol-S (BPS) is a new bisphenol-A substitute widely used in many plastic products. Bisphenol-A as a main member of bisphenol family has been known as an endocrine system disrupter chemical compound. Like other members of bisphenol family, there is public health concern about the toxic effects of BPS on reproductive system, thus, we examined BPS effects on in vitro fertilization (IVF) potential and oxidative stress status in a murine model. Adult female mice (n = 70) were randomly divided into control and BPS-treated groups. Bisphenol-S was administered at doses of 0, 1, 5, 10, 50 and 100 µg kg-1 body weight per day intraperitoneally for 21 consecutive days. Twenty-Four hr after the last treatment, five mice in each group were super-ovulated and the oocytes were harvested for IVF. All ovaries were collected and used for biochemical factors analyses. Bisphenol-S exposure at doses more than 10 µg kg-1 induced developmental arrest of pre-implantation embryos. Further, lipid peroxidation measurement in ovaries indicated that all doses of BPS cause oxidative stress in female mice. In conclusion, BPS administration even in low doses can result in female reproductive toxicities and oxidative stress in mice.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
341
345
https://vrf.iranjournals.ir/article_28488_4209ec6a913ea8cbf87a78b98f67e6ad.pdf
Molecular detection of Ehrlichia spp. in blood samples of dogs in southern Iran using polymerase chain reaction
Noushin
Derakhshandeh
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Hassan
Sharifiyazdi
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
Mohammad
Abbaszadeh Hasiri
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
author
text
article
2017
eng
Ehrlichiosis is a zoonotic disease which has been reported from some regions of Iran. This study was aimed to determine the presence and prevalence of ehrlichiosis in suspected dogs referred to the Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran using polymerase chain reaction(PCR). Blood samples were collected from 98 suspected dogs with at least one of the five following findings: thrombocytopenia, anemia (hematocrit < 37.00%), gastrointestinal signs and respiratory and/or central nervous system diseases. Complete blood count was performed for each sample. After genomic DNA extraction, PCR assay was carried out using a commercial PCR kit. The results showed that only three out of 98 samples (3.06%) were positive for ehrlichiosis. There was no significant difference in hematological parameters between infected and non-infected cases. These results emphasize that ehrlichiosis has a low prevalence among examined cases in southern Iran. Further serological and molecular studies are needed to clarify the epidemiological feature of this infection in different areas of Iran.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
347
351
https://vrf.iranjournals.ir/article_28489_3b70295027b43496b275ccc26340e107.pdf
Effect of different levels of dietary vitamin E on reproductive and productive performances in Japanese quails (Coturnix coturnix japonica)
Parvin
Abedi
Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resource University of Khuzestan, Mollasani, Ahvaz, Iran
author
Saleh
Tabatabaei Vakili
Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resource University of Khuzestan, Mollasani, Ahvaz, Iran
author
Morteza
Mamouei
Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resource University of Khuzestan, Mollasani, Ahvaz, Iran
author
Ali
Aghaei
Department of Animal Science, Faculty of Animal Science and Food Technology, Ramin Agriculture and Natural Resource University of Khuzestan, Mollasani, Ahvaz, Iran
author
text
article
2017
eng
The aim of this study was to evaluate the effects of dietary vitamin E on reproductive and productive parameters in Japanese quails. A total number of 240 female and 80 male Japanese quail were divided into five treatments with four replications in a completely randomized design. Experimental diets were zero control, 30, 60, 120 and 240 mg kg-1 of vitamin E. Fertility and total hatchability were not affected by treatments. But, the lowest hatch of fertile eggs and the highest embryonic death were observed in control group (p < 0.05). Left testes weight in T2 and T4 was higher than control (p < 0.05). Right testes weight and sera FSH concentration in males were not affected by treatments. The highest testosterone concentration of males was observed in T5 (p < 0.05). Weight and length of oviduct as well as weight of ovary and FSH concentration in females did not affected by treatments. Estrogen concentration in T4 treatment was greater than control (p < 0.05). Most of the egg characteristics were not affected by treatments. However, higher egg mass and production rates were observed in T2 and T3groups than control group (p < 0.05). Feed intake and conversion ratio did not differ among treatments. In conclusion, dietary vitamin E improved hatch of fertile egg, embryonic viability, egg mass and production rates in Japanese quail. The effect of dietary vitamin E combined with selenium on these characteristics are recommended for future study in Japanese quail.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
353
359
https://vrf.iranjournals.ir/article_28484_f4dbf94b004af66dbb99c5e827831ca5.pdf
Congenital lumbar vertebrae agenesis in a lamb
Mohammad
Farajli Abbasi
Post Graduate Student of Veterinary Surgery, Shahid Bahonar University of Kerman, Kerman, Iran
author
Bahador
Shojaei
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
Omid
Azari
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
author
text
article
2017
eng
Congenital agenesis of lumbar vertebrae was diagnosed in a day-old female lamb based on radiology and clinical examinations. There was no neurological deficit in hindlimb and forelimb associated with standing disability. Radiography of the abdominal region revealed absence of lumbar vertebrae. Necropsy confirmed clinical and radiographic results. No other anomaly or agenesis was seen macroscopically in the abdominal and thoracic regions as well as vertebral column. Partial absence of vertebral column has been reported in human and different animal species, as an independent occurrence or associated with other organs anomalies. The latter has been designated as caudal regression syndrome. Vertebral agenesis may arise from irregularity in the differentiation of somites to the sclerotome or sclerotome to the vertebral primordium. Most of the previously reported cases of agenesis were related to the lumbosacral region, lonely or along with other visceral absences. This case was the first report of congenital agenesis of lumbar vertebrae in a lamb.
Veterinary Research Forum
Faculty of Veterinary Medicine, Urmia University
2008-8140
8
v.
4
no.
2017
361
363
https://vrf.iranjournals.ir/article_28490_ae6a6791bff6f2345e0a29f6b908f9cc.pdf