@article { author = {Mard, Seyyed Ali and Mojadami, Shahnaz and Farbood, Yaghoob and Gharib Naseri, Mohammad Kazem}, title = {The anti-inflammatory and anti-apoptotic effects of gallic acid against mucosal inflammation- and erosions-induced by gastric ischemia-reperfusion in rats}, journal = {Veterinary Research Forum}, volume = {6}, number = {4}, pages = {305-311}, year = {2015}, publisher = {Faculty of Veterinary Medicine, Urmia University}, issn = {2008-8140}, eissn = {2322-3618}, doi = {}, abstract = {The present study aimed to evaluate the protective effect of gallic acid on gastric mucosal lesions caused by ischemia-reperfusion (I/R) injury in rat. Forty male rats were randomly divided into sham, control (I/R injury) and three gallic acid-pretreated groups. To induce I/R lesions, the celiac artery was clamped for 30 min and then the clamp was removed to allow reperfusion for 6 hr. Pretreated rats received gallic acid (15, 30 or 60 mg kg-1, intraperitoneally) 30 min prior to the induction of I/R injury. Macroscopic and microscopic evaluations of the areas of ulceration were compared. Samples of gastric mucosa were collected to evaluate the protein expression of pro-apoptotic factor, caspase-3, and pro-inflammatory enzyme, inducible nitric oxide synthase (iNOS) using western blot. Pretreatment with gallic acid decreased the total area of gastric lesions. Gallic acid at 30 mg kg-1 decreased the levels of protein expression of caspase-3 and iNOS induced by I/R injury. Our findings showed the protective effect of gallic acid on gastric mucosa against ischemia-reperfusion injury. This effect of gallic acid was mainly mediated by reducing protein expression of iNOS and caspase-3.}, keywords = {Caspase-3,Gallic acid,Inducible nitric oxide synthase,Ischemia-reperfusion injury,Rat}, url = {https://vrf.iranjournals.ir/article_14945.html}, eprint = {https://vrf.iranjournals.ir/article_14945_5df45c7aec560c1a5c5598146147b030.pdf} }