@article { author = {Vargas Ibarra, Ana Karen and Carcoba Pérez, Samantha Anahi and Avalos Rodríguez, Alejandro and Rosales Torres, Ana María and Rodriguez-Hernandez, Fernanda and Camarillo Flores, Ricardo and Quintana López, José Antonio and Herrera Barragán, José Antonio}, title = {In vitro sperm storage with poultry oviductal secretions}, journal = {Veterinary Research Forum}, volume = {11}, number = {3}, pages = {207-211}, year = {2020}, publisher = {Faculty of Veterinary Medicine, Urmia University}, issn = {2008-8140}, eissn = {2322-3618}, doi = {10.30466/vrf.2019.95854.2300}, abstract = {In the hen oviduct, tubules have been identified that preserve the sperm, maintaining viability for up to 15 weeks. This study aimed to evaluate the physiological status of rooster sperm when preserved in vitro with uterus vaginal junction secretions (UVJS). Males and females of the Rhode Island breed were used. Sperm aliquots were prepared using Lake extender and Lake extender with UVJS (10.00%, 30.00%, 60.00%, and 90.00%). Subsequently, a basic sperm evaluation was performed and sperm physiological status was determined through the presence and distribution of Ca2+ and its acrosomal reaction capability via perivitelline layer (PVL) co-incubation. It was observed that motility was decreased in sperm preserved with UVJS at 6 and 24 hr) compared to 40 min and fresh semen. The sperm decapacitation percentage was increased when preserved with UVJS at 40 min, 6 and 24 hr compared to fresh semen. The acrosomal reaction was increased in sperm co-incubated with PVL, even when preserved with UVJS. It was concluded that UVJS induced physiological changes in sperm by inducing a decapacitation process, which increased sperm viability when preserved in vitro.}, keywords = {Acrosome,decapacitation,Hen,oviduct,Semen}, url = {https://vrf.iranjournals.ir/article_40415.html}, eprint = {https://vrf.iranjournals.ir/article_40415_e59dd78f0e15c09a2eaa4001467a679c.pdf} }