%0 Journal Article %T Molecular characterization and phylogenetic analysis of foot and mouth disease virus isolates in Sulaimani province, Iraq %J Veterinary Research Forum %I Faculty of Veterinary Medicine, Urmia University %Z 2008-8140 %A Baba Sheikh, Mohammed %A Atta Rashid, Peshnyar %A Raheem, Zhino %A Salahadin Marouf, Ari %A Mohammed Amin, Kamaran %D 2021 %\ 06/15/2021 %V 12 %N 2 %P 247-251 %! Molecular characterization and phylogenetic analysis of foot and mouth disease virus isolates in Sulaimani province, Iraq %K Molecular genotyping %K Phylogenetic analysis %K viral evolution %K VP1 gene %R 10.30466/vrf.2019.101755.2424 %X Foot-and-mouth disease (FMD) is endemic in Iraq. The current study can be considered as the first molecular characterization of serotype O in Iraq. The present investigation reported the determination of FMDV serotype O from local farms in Sulaimani districts in 2016 outbreaks. Samples were collected from suspected cattle. The virus was primarily detected with RT-PCR directly from mouth epithelial samples. The direct sequencing and subsequent analysis of amplified PCR products for the VP1 gene indicated the circulation of serotype O of FMD in studied areas. Moreover, phylogenetic analysis revealed that the field isolated serotype O belonged to topotype ME-SA and lineage PanAsia II and clustered with Pakistan and Iran isolates (KU365843 and KY091283) with identity (96.00%, 95.00%) respectively. Furthermore, according to the phylogenic tree, the field isolates had different lineage with the three O/Manisa vaccine strains and Iraq/2000 strains. These findings highlighted the continuous circulation of serotype O of FMD in the region. %U https://vrf.iranjournals.ir/article_243060_79b48d4738e9c683719e6f858ffabf5e.pdf