%0 Journal Article %T DNA damage in dental pulp mesenchymal stem cells: An in vitro study %J Veterinary Research Forum %I Faculty of Veterinary Medicine, Urmia University %Z 2008-8140 %A Aramburú Junior, Jaime Sardá %A Treichel, Tiago Luis Eilers %A Pinto Filho, Saulo Tadeu Lemos %A Gerhke, Sergio Alexandre %A Kolinski Machado, Alencar %A Carla Cadoná, Francine %A Mânica da Cruz, Ivana Beatrice %A Pippi, Ney Luis %D 2018 %\ 12/15/2018 %V 9 %N 4 %P 293-299 %! DNA damage in dental pulp mesenchymal stem cells: An in vitro study %K Dental pulp %K DNA damage %K Genotoxicity %K Mesenchymal stem cell %K Oxidative stress %R 10.30466/vrf.2018.33083 %X The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for clinical application of cellular therapy for this type of cell. The cell culture was maintained with Dulbecco’s modified Eagle’s medium supplemented with 10.00% fetal bovine serum for eight passages. During each passage, cell proliferation, oxidative stress and level of DNA fragmentation were assessed by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, testing 2,7 dichlorodihydro-fluorescein-diacetate and PicoGreen®, respectively. There were important differences among the first three DPSC passages compared to passages 4–8 and a large number of nuclei with some levels of DNA damage (30.00 to 40.00% in initial DPSC passages and > 50.00% in late passages), indicating in vitro DPSC genomic fragility. Within the limitations of this study, the results suggest these relatively simple and inexpensive approaches - comet and DNA fragmentation assays - could help sort stem cells with less DNA damage for use in research or therapies. %U https://vrf.iranjournals.ir/article_33083_c01bfc358eca9ab478a456a2c88d0ff7.pdf