ORIGINAL_ARTICLE
Tongue structure in the long-eared hedgehog (Hemiechinus auritus): A scanning electron microscopic study
The aim of this study was to investigate the dorsal surface of the long-eared hedgehog tongue using scanning electron microscopy. The tongue of the long-eared hedgehog is rectangular-shaped with an elongated body and a widened root and a marked deep median groove can be seen on its dorsal surface. A characteristic feature of the lingual root is presence of two huge laterally situated folds. These anatomical structures have never been described with regard to other small mammals studied so far. According to their anatomical appearances, the lingual papillae can be distinguished as filiform, conical, fungiform, circumvallate and foliate papillae. The filiform papillae covering the body of the tongue are longer and wider than those on its apex and have an apparent fork-like appearance. Fungiform papillae are evenly distributed on the dorsal surface of the apex and body of the tongue. The triangular area of the lingual root contains small caudally directed conical papillae with single processes. Foliate papillae are situated on both lateral surfaces of the lingual root medial to the huge lateral lingual folds as three large obliquely situated parallel folds. There are three circumvallate papillae, two of which are obliquely situated on both sides of the rostral part of the lingual root, while the third one is situated in the midline of the caudal part of the lingual root. The results show that the tongue structure of the long-eared hedgehog is more complex in comparison with other mammals which is related to its phylogeny and feeding habits.
https://vrf.iranjournals.ir/article_32078_09c939cd1c61c0a60bee2702cc1f0481.pdf
2018-09-01
205
209
10.30466/vrf.2018.32078
Lingual papillae
Long-eared hedgehog
Scanning electron microscope
Tongue
Ali
Parchami
parchami@vet.sku.ac.ir
1
Department of Basic Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
LEAD_AUTHOR
Mahdi
Salimi
salimi412@yahoo.com
2
Graduate Student of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
AUTHOR
Mehrdad
Khosravi
3
DVSc Graduated of Avian Diseases, Young Researchers and Elite Club, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
AUTHOR
Iwasaki S. Evolution of the structure and function of the vertebrate tongue. J Anat 2002; 201(1): 1-13.
1
Emura S, Okumura T, Chen H. Scanning electron microscopic study of the tongue in the peregrine falcon and common kestrel. Okajimas Folia Anat Jpn 2008; 85(1): 11-15.
2
Emura S, Okumura T, Chen H. Scanning electron microscopic study of the tongue in the Japanese pygmy woodpecker (Dendrocopos kizuki). Okajimas Folia Anat Jpn 2009; 86(1): 31-35.
3
Sonntag CF. The comparative anatomy of the tongues of the mammalia. X. Rodentia. Proc Zool Soc London 1924; 95(2): 725-748.
4
Fawcett DW. Textbook of histology. Philadelphia, USA: WB Saunders 1986:112-117.
5
Jabbar AI. Anatomical and histological study of tongue in the hedgehog (Hemiechinus auritus). Int J Recent Sci Res 2014; 5(4): 760-763.
6
Taha AM. Comparative anatomical, histological and histochemical study of tongue in two species of insectivorous vertebrates. Aust J Basic Appl Sci 2013; 7(1): 401-410.
7
Grandi D, Arcari ML, Azalli G. Ultrastructural aspects of the lingual papillae in the gerbil (Meriones unguiculatus). Ital J Anat Embryol 1994; 99: 201-217.
8
Iwasaki S, Yoshizawa H, Kawahara I. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the mouse. Anat Rec 1996; 157(1): 41-52.
9
Iwasaki S, Yoshizawa H, Kawahara I. Study by scanning electron microscopy of the morphogenesis of three types of lingual papilla in the rat. Anat Rec 1997; 247(4): 528-541.
10
Kobayashi K. Three-dimensional architecture of the connective tissue core of the lingual papillae in the guinea pig. Anat Embryol 1990; 182: 205-213.
11
Abumandour MMA, El-Bakary RMA. Morphological and scanning electron microscopic studies of the tongue of the Egyptian fruit bat (Rousettus aegyptiacus) and their lingual adaptation for its feeding habits. Vet Res Commun 2013; 37(3): 229-238.
12
Thome H. Oral cavity and pharynx. In: Nickel R, Schummer A, Seiferle E (Eds). Textbook of the anatomy of pets. Berlin, Germany: Parey Buchverlag 1971; 121-153.
13
Abumandour MMA, EL-Bakary RMA. Anatomical reference for morphological and scanning electron microscopic studies of the New Zealand white rabbits tongue (Orycotolagus cuniculus) and their lingual adaptation for feeding habits. J Morphol Sci 2013; 30(4): 254-365.
14
Iino T, Kobayashi K. Morphological studies on the lingual papillae and their connective tissue papillae of rats. Shigaku 1988; 75(6): 1039-1060.
15
Jackowiak H, Godynicki S. The distribution and structure of the lingual papillae on the tongue of the bank vole Clethrinomys glareolus. Folia Morphol (Warsz) 2005; 64(4): 326-333.
16
Wolczuk K. Dorsal surface of the tongue of the hazel dormouse (Muscardinus Avellanarius): Scanning electron and light microscopic study. Zoolog Pol 2014; 59(1-4): 35-47.
17
Emura S, Tamada A, Hayakawa D, et al. SEM study on the dorsal lingual surface of the flying squirrel, Petaurista leucogenys. Ann Anat 1999; 181(5): 495-498.
18
Dinc G, Girgin A, Yilmaz S. Prenatal and postnatal development of papilla fungiformis in rats [Turkish]. F Ü Saðlik Bil Derg 1995; 9: 161-163.
19
Emura S, Tamada A, Hayakawa D, et al. SEM study on the dorsal lingual surface of the nutria, Myocastor coypus. Kaibokagu Zasshi 2001; 76(2): 233-238.
20
Kobayashi K, Miyata K, Takahashi K, et al. Three-dimensional architecture of the connective tissue papillae of the mouse tongue as viewed by scanning electron microscopy. Kaibogaku Zasshi 1989; 64(6): 523-538.
21
Emura S, Okumura T, Chen H, et al. Morphology of the lingual papillae in the raccoon dog and fox. Okajimas Folia Anat Jpn 2006; 83(3): 73-76.
22
Emura S, Okumura T, Chen H. Morphology of the lingual papillae in the Japanese marten. Okajimas Folia Anat Jpn 2004; 84(2): 77-82.
23
Pastor J, Moro J, Verona J, et al. Morphological study by scanning electron microscopy of the lingual papillae in the common European bat (Pipistrellus pipistrellus). Arch Oral Biol 1993; 38(7): 597-599.
24
Shindo J, Yoshimura K, Kobayashi K. Comparative morphological study on the stereo-structure of the lingual papillae and their connective tissue cores of the American beaver (Castor canadensis). Okayima Folia Anat Jpn 2006; 82(4): 127-138.
25
ORIGINAL_ARTICLE
ECOR phylotyping and determination of virulence genes in Escherichia coli isolates from pathological conditions of broiler chickens in poultry slaughter-houses of southeast of Iran
Avian pathogenic Escherichia coli (APEC) are responsible for wide ranges of extra-intestinal diseases in poultry including colibacillosis, cellulitis, coligranuloma and yolk sac infection. Numbers of virulence are considered important in the pathogenicity of these diseases. The aims of the present study were phylogenetic typing and virulence genes detection in Escherichia coli isolates from colibacillosis and cellulitis of broiler chickens in poultry slaughterhouses of Shahrbabak region, Kerman, Iran. A total number of eighty three E. coli isolates were taken from broiler chickens with colibacillosis and thirty four isolates were taken from carcasses with cellulitis in the industrial slaughterhouses. Biochemically confirmed E. coli isolates were subjected to polymerase chain reaction assay to determine phylogenetic groups and presence of pap C, sfa/focDE, iucD, afaIB-C, hlyA, fimH and crl virulence genes. Colibacillosis isolates were belonged to A (54.21%), B1 (7.22%), B2 (6.03%) and D (32.53%) phylogroups. Whereas, the isolates from cellulitis cases were belonged to three main phylogroups; A (55.88%), B1 (5.88%) and D (38.24%). Statistical analysis showed a specific association between the presence of crl virulence gene and phylogroups of A and D in colibacillosis isolates. The results showed that the isolates from both diseases in broiler chickens could be assigned to various phylogenetic groups (mainly A(. Also, the virulence genes profile of cellulitis E. coli is completely different from that of colibacillosis in this region.
https://vrf.iranjournals.ir/article_30827_8a17f3b0772a5f06c4549f266516d054.pdf
2018-09-01
211
216
10.30466/vrf.2018.30827
Cellulitis
Colibacillosis
Escherichia coli
Phylogenetic group
Virulence genes
Akbar
Asadi
mnasernazem@gmail.com
1
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Taghi
Zahraei Salehi
tzahraei@vetmed.ut.ac.ir
2
Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Mahmoud
Jamshidian
jamshidianm24@yahoo.com
3
Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
AUTHOR
Reza
Ghanbarpour
ghanbar@uk.ac.ir
4
Molecular Microbiology Research Group, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
LEAD_AUTHOR
Clermont O, Bonacorsi S, Bingen E. Rapid and simple determination of the Escherichia coli phylogenetic group. Appl Environ Microbiol 2010; 66: 4555-4558.
1
Saif YM, Fadly AM, Glisson JR, et al. Diseases of poultry. 12th ed. London, UK: Blackwell 2008;121-123.
2
Johnson JR, Murray AC, Gajewski A, et al. Isolation and molecular characterization of nalidixic acid-resistant extra-intestinal pathogenic Escherichia coli from retail chicken products. Antimicrob Agents Chemother 2003; 47: 2161-2168.
3
Roy P, Purushothaman V, Koteeswaran A, et al. Isolation, characterization, and antimicrobial drug resistance pattern of Escherichia coli isolated from Japanese quail and their environment. J Appl Poultry Res 2006; 15(3): 442-446.
4
de Brito BG, Gaziri LC, Vidotto MC. Virulence factors and clonal relationships among Escherichia coli isolates isolated from broiler chickens with cellulitis. Infect Immun 2003; 71:4175-4177.
5
Ghanbarpour R, Salehi M, Oswald E. Virulence genotyping of Escherichia coli isolates from avian cellulitis in relation to phylogeny. Comp Clin Pathol 2010; 19:147-153.
6
de Campos TA, Stehling EG, Ferreira A, et al. Adhesion properties, fimbrial expression and PCR detection of adhesin-related genes of avian Escherichia coli isolates. Vet Microbiol 2005; 106(3): 275-285.
7
Kawano M, Yaguchi K, Osawa R. Genotypic analyses of Escherichia coli isolated from chickens with colibacillosis and apparently healthy chickens in Japan. Microbiol Immunol 2006; 50: 961-966.
8
Jafari RM, Motamedi H, Maleki E, et al. Phylogenetic typing and detection of extended-spectrum β-lactamases in Escherichia coli isolates from broiler chickens in Ahvaz, Iran. Vet Res Forum 2016; 7(3): 227-233.
9
Mohsenifard E, Asasi K, Sharifiyazdi H, et al. Phylotyping and ColV plasmid-associated virulence genotyping of E. coli isolated from broiler chickens with colibacillosis in Iran. Com Clin Patho 2016; 25: 1035-1042.
10
Ghanbarpour R, Askari N, Ghorbanpour M, et al. Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran. Trop Anim Health Prod 2017; 49(3):591-597.
11
Alizadeh H, Ghanbarpour R, Jajarmi M, et al. Phylogenetic typing and molecular detection of virulence factors of avian pathogenic Escherichia coli isolated from colibacillosis cases in Japanese quail. Vet Res Forum 2017; 8(1):55-58.
12
Yamamoto S, Terai A, Yuri K, et al. Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunol Med Microbiol 1995; 12(2): 85-90.
13
Van Bost S, Jacquemin E, Oswald E, et al. Multiplex PCRs for identification of necrotoxigenic Escherichia coli. J Clin Microbiol 2003; 41:4480-4482.
14
Dissanayake DR, Wijeuardana TG, Gunawardena G, et al. Distribution of lipopolysaccharide core type among avian pathogenic E. coli in relation to the major phylogenetic groups. Vet Microbiol 2008; 132(3-4):355-363.
15
Ewers C, Li G, Wilking H, et al. Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: How closely related are they? Int J Med Microbiol 2007; 297: 163-176.
16
Mellata M. Human and avian extra-intestinal pathogenic Escherichia coli: Infections, zoonotic risks, and antibiotic resistance trends. Foodborne Pathog Dis 2013; 10(11): 916-932.
17
Mora A, Viso S, López C, et al. Poultry as reservoir for extra-intestinal pathogenic Escherichia coli O45:K1:H7-B2-ST95 in humans. Vet Microbiol 2013; 167(3-4): 506-512.
18
Li G, Laturnus C, Ewers C, et al. Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun 2005; 73: 2818-2827.
19
La Ragione RM, Woodward MJ. Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia. Res Vet Sci 2002; 73: 27-35.
20
Mokady D, Gophna U, Ron EZ. Virulence factors of septicemic Escherichia coli isolates. Int J Med Microbiol 2005; 295(6-7); 455-462.
21
Delicato ER, de Brito BG, Gaziri LC, et al. Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis. Vet Microbiol 2003; 94: 97-103.
22
Dozois CM, Fairbrother JM, Harel J, et al. pap- and pil-related DNA sequences and other virulence determinants associated with Escherichia coli isolated from septicemic chickens and turkeys. Infect Immun 1992; 60: 2648-2656.
23
Jeffrey JS, Nolan LK, Tonooka KH, et al. Virulence factors of Escherichia coli from cellulitis or colisepticemia lesions in chickens. Avian Dis 2002; 46(1): 48-52.
24
ORIGINAL_ARTICLE
Comparative study of the protective effects of chicken embryo amniotic fluid, vitamin C and coenzyme Q10 on cyclophosphamide-induced oxidative stress in mice ovaries
Cyclophosphamide is a chemotherapy drug for the treatment of cancer. Chicken embryo amniotic fluid, vitamin C and coenzyme Q10 have anti-oxidant properties. Total of 70 adult female mice were selected and divided into seven groups. The first group that received 2 ml kg-1 of inactivated amniotic fluid subcutaneously. The second group treated with 75 mg kg-1of cyclophosphamide by intraperitoneal injection. Third to fifth groups received 1, 2, and 4 ml kg-1 of chicken embryo amniotic fluid, respectively. The sixth group received vitamin C at a dose of 0.2 mg g-1 of body weight by oral gavages. Seventh group received 10 mg kg-1 coenzyme Q10 intraperitoneally. All cyclophosphamide treated groups (3-7) received 75 mg kg-1 of cyclophosphamide intraperitoneal on day 22. The mice were euthanized on day 29 and ovarian tissue antioxidant enzymes including glutathione peroxidase, superoxide dismutase and catalase activities and malondialdehyde (MDA) were evaluated. Activities of above mentioned enzymes in treatment groups (3-7) was significantly higher than patient control group (2). The results also revealed that MDA levels were higher in the control group in comparison to other treatment groups. Therefore, it is concluded that the chick embryo amniotic fluid and coenzyme Q10 can compete with compounds like vitamin C in increasing the anti-oxidant level in ovarian tissue.
https://vrf.iranjournals.ir/article_32085_49d87f3a8e24162481e6158ee8344629.pdf
2018-09-01
217
224
10.30466/vrf.2018.32085
Chicken embryo amniotic fluid
Coenzyme Q10
Cyclophosphamide
Oxidative stress
Vitamin C
Alireza
Kabirian
1
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Rooz Ali
Batavani
alibatavani@yahoo.com
2
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
LEAD_AUTHOR
Siamak
Asri-Rezaei
siamak.asri@gmail.com
3
Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Ali
Soleimanzadeh
4
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Hoyer PB, Sipes IG. Assessment of follicle destruction in chemical-induced ovarian toxicity. Annu Rev Pharmacol Toxicol 1996; 36: 307-331.
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Jarrell JF, Bodo L, YoungLai EV, et al. The short-term reproductive toxicity of cyclophosphamide in the female rat. Reprod Toxicol 1991; 5(6): 481-485.
2
Tomao F, Spinelli GP, Panici PB, et al. Ovarian function, reproduction and strategies for fertility preservation after breast cancer. Crit Rev Oncol Hematol 2010; 76(1): 1-12.
3
Jiang Y, Zhao J, Qi H, et al. Accelerated ovarian aging in mice by treatment of busulfan and cyclophosphamide. J Zhejiang Univ Sci B 2013; 14(4): 318-324.
4
Plowchalk M, Mattison DR. Reproductive toxicity of cyclophosphamide in the C57BL/6N mouse: 1. Effects on ovarian structure and function. Reprod Toxicol 1992; 6(5): 411-421.
5
Eman A, Kishk F, Mona HM. Effect of a gonadotropin-releasing hormone analogue on cyclophosphamide -induced ovarian toxicity in adult mice. Arch Gynecol Obstet 2013; 287(5): 1023-1029.
6
Tsai-Turton M, Luong BT, Tan Y, et al. Cyclo-phosphamide-induced apoptosis in COV434 human granulosa cells involves oxidative stress and glutathione depletion. Toxicol Sci 2007; 98(1): 216-230.
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Fujii J, Iuchi Y, Okada F. Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system. Reprod Biol Endocrinol 2005; 3: 43.
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Tilly JL, Tilly KL. Inhibitors of oxidative stress mimic the ability of follicle-stimulating hormone to suppress apoptosis in cultured rat ovarian follicles. Endocrinology 1995; 136(1): 242-252.
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Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3(28): 1-29.
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Larsen WJ. Human embryology. 3rd ed. Philadelphia, USA: Churchill Livingstone 2001; 490.
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Burdett P, Lizana J, Eneroth P, et al. Proteins of human amniotic fluid. II. Mapping by two-dimensional electrophoresis. Clin Chem 1982; 28:935-940.
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Batavani RA, Ghasemzadeh M, Kheradmand A. Changes of enzyme activities in ovine fetal fluids and maternal blood serum with gestational age. Comp Clin Pathol 2008; 17: 105-109.
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Kaya M, Ozkan A, Eroz R, et al. Effect of combined anti-inflammatory and antioxidant therapy on ischemia-reperfusion injury in rat ovary: An experimental study. Int J Clin Exp Med 2016; 9(3): 6246-6254.
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Mashayekhi F, Dianati E, Masomi Moghadam L. Quantitative analysis of nerve growth factor in the amniotic fluid during chick embryonic development. Saudi J Biol Sci 2011; 18(2): 209-212.
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Mirzajani E, Dejhagah S, Mashayekhi F, et al. Amniotic fluid TGF-β1 concentration during chick embryonic development. Ann Biol Res 2011; 2(5): 185-190.
16
Mashayekhi F, Zahiri S, Mirzajani E, et al. Developmental changes in amniotic fluid vascular endothelial growth factor levels of chick embryos. Ann Biol Res 2011; 2(1): 94-99.
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Karcher DM, McMurtry JP, Applegate TJ. Developmental changes in amniotic and allantoic fluid insulin-like growth factor (IGF)-I and -II concentrations of avian embryos. Comp Biochem Physiol A Mol Integr Physiol 2005;142(4):404-409.
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Busch M, Milakofsky L, Hare T, et al. Regulation of substances in allantoic and amniotic fluid of the chicken embryo. Comp Biochem Physiol 1997; 116(2): 131-136.
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Pressman EK, Thornburg LL, Glantz JC, et al. Inflammatory cytokines and antioxidants in midtrimester amniotic fluid: Correlation with pregnancy outcome. Am J Obstet Gynecol 2011; 204(2): 155.e1-7.
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Bartosz G. Total antioxidant capacity. Adv Clin Chem 2003; 37: 219-292.
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Sarala P, Suneetha S, Parthasarathy PR. Detection of superoxide dismutase isozymes in normal and X-irradiated chick amniotic fluid by using isoelectric focusing. Indian J Exp Biol 1996; 34(9): 901-904.
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May JM. How does ascorbic acid prevent endothelial dysfunction? Free Radic Biol Med 2000; 28: 1421-1429.
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Gasparetto C, Malinverno A, Gulacciati D, et al. Antioxidant vitamins reduce oxidative stress and ventricular remodeling in patients with acute myocardial infarction. Int J Immunopathol Pharmacol 2005; 18(3): 487-496.
24
Mohamed R, Dharmappa KK, Tarannum S, et al. Chemical modification of ascorbic acid and evaluation of its lipophilic derivatives as inhibitors of secretory phospholipase A2 with anti-inflammatory activity. Mol Cell Biochem 2010; 345(1-2): 69-76.
25
Hosseinzadeh E, Zavareh S, Lashkarboluki T. Coenzyme Q10 improves developmental competence of mice pre-antral follicle derived from vitrified ovary. J Paramed Sci 2015; 6(2): 65-71.
26
Turi A, Giannubilo SR, Brugè F, et al. Coenzyme Q10 content in follicular fluid and its relationship with oocyte fertilization and embryo grading. Arch Gynecol Obstet 2012; 285(4): 1173-1176.
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Bonakdar RA, Guarneri E. Coenzyme Q10. Am Fam Physician 2005; 72(6):1065-1070.
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Farjah GH, Fazli F. The effect of chick embryo amniotic fluid on sciatic nerve regeneration of rats. Iran J Vet Res 2015; 16(2): 167-171.
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Hamburger V, Hamilton HL. A series of normal stages in the development of the chick embryo. 1951.Dev Dyn 1992; 195(4): 231-272
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Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: The FRAP assay. Anal Biochem 1996; 239(1): 70-76.
31
Jalali AS, Najafi G, Hosseinchi M, et al. Royal jelly alleviates sperm toxicity and improves in vitro fertilization outcome in stanozolol-treated mice. Iran J Reprod Med 2015; 13(1): 15-22.
32
Yapca OE ,Turan MI, Borekci B, et al. Bilateral ovarian ischemia/reperfusion injury and treatment options in rats with an induced model of diabetes. Iran J of Basic Med Sci 2014; 17(4): 294-302.
33
Hadwan MH, Abed HN. Data supporting the spectrophotometric method for the estimation of catalase activity. Data Brief 2016; 6: 194-199.
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Surai PF. Antioxidant systems in poultry biology: Superoxide dismutase. J Anim Res Nutr 2015; 1:8. doi: 10.21767/2572-5459.100008.
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Senthilkumar S, Yogeeta SK, Subashini R, et al. Attenuation of cyclophosphamide induced toxicity by squalene in experimental rats. Chem-bioloint 2006; 160(3): 252-260.
36
Mohammadi T, Khaksary Mahabadi M, Najaf-ZadeVarzi H, et al. Protective effect of concurrent administration of mesna and galbanum oil on histological structure of rat placenta against cyclophosphamide toxicity. J Ardabil Uni Med Sci 2016; 16(2): 211-222.
37
Yener NA, Sinanoglu O, Ilter E, et al. Effects of spirulina on cyclophosphamide-induced ovarian toxicity in rats: Biochemical and histomorphometric evaluation of the ovary. Biochem Res Int 2013; 2013: http://dx.doi.org/ 10.1155/2013/764262.
38
Laili AN, Ananingsih L, Wiyasa WA, et al. Protective effect of combined vitamin C and E against ovarian and endometrial toxicity in rats that receiving oral rhodamine B. Biomarkers Genomic Med 2015; 7(4): 154-158.
39
Ismiyati A, Wiyasa WA, Hidayati DYN. Protective effect of vitamins C and E on depot-medroxyprogesterone acetate-induced ovarian oxidative stress in vivo. J Toxicol 2016; doi: 10.1155/2016/3134105.
40
Tuncer AA, Bozkurt MF, Koken T, et al. The protective effects of alpha-lipoic acid and coenzyme Q10 combination on ovarian ischemia-reperfusion injury: An experimental study. Adv Med. 2016;2016: doi: 10.1155/2016/3415046.
41
Ozler A, Turgut A, Görük NY, et al. Evaluation of the protective effects of CoQ₁₀ on ovarian I/R injury: An experimental study. Obstet Gynecol Sci Investigation 2013; 76(2): 100-106.
42
Dai XX, Duan X, Cui X-S, et al. Melamine induces oxidative stress in mouse ovary. J PLoS One 2015; 10(11):1-9.
43
Sekhon L, Gupta S, Kim Y, et al. Female infertility and antioxidants. Curr Womens Health Rev 2010; 6(2): 85-95.
44
Laloraya M, Kumar GP, Laloraya MM. Histochemical study of superoxide dismutase in the ovary of the rat during the oestrous cycle. J Reprod Fertil 1989; 86(2): 583-587.
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Matzuk MM, Dionne L, Guo Q, et al. Ovarian function in superoxide dismutase 1 and 2 knockout mice. Endocrinology 1998; 139(9): 4008-4011.
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Enders AC, Nelson DM. Pinocytotic activity of the uterus of the rat. Dev Dyn J 1973; 138(3): 277-299.
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Skrajnowska D, Bobrowska-Korczak B, Tokarz A, et al. Copper and resveratrol attenuates serum catalase, glutathione peroxidase, and element values in rats with DMBA-induced mammary carcinogenesis. Biol Trace Elem Res 2013; 156(1-3): 271-278.
49
ORIGINAL_ARTICLE
Chemical disbudding of goat kids with subcutaneous administration of synthetic eugenol: Histopathology and morphometry
This study was conducted to evaluate the effect of subcutaneous administration of synthetic eugenol (EG) for disbudding of goat kids, as a new chemical method. Thirty apparently healthy Raieni (Cashmere) goat kids (five-day-old) were divided randomly into six groups (n = 5). In the pathology (P) groups (P3, P8 and P60 according to the sample collection day after injection) an amount of 0.10 mL of EG was subcutaneously administrated in both horn buds. In the disbudding 1 and 2 (DB1, DB2) groups, 0.10 mL of EG, and in the control (C) group 0.10 mL normal saline was subcutaneously injected in the right horn buds, respectively. Eugenol injection in DB2 group was done in twelve-day-old goat kids. The left horn buds of DB1, DB2 and C groups were considered as control of horn outgrowing. The horn buds, kidneys, liver, lung, brain and heart, tissue specimens were collected from P3 and P8 groups, and bud skin samples were collected from P60 group. The results showed that the EG was able to stop the horn growth in the first week of goat life. There was no significant difference between left and right horn size in the C group. Histopathological study revealed complete necrosis of bud tissue in dermal and epidermal layers, in P3 animals. Healing and re-epithelialization were seen in the samples taken from P8 group. Subcutaneous injection of the synthetic EG can be considered as a new method for goat kids disbudding.
https://vrf.iranjournals.ir/article_32084_6ceaf8337f2cf877505d4b3302c81c7a.pdf
2018-09-15
225
230
10.30466/vrf.2018.32084
Disbudding
Goat kid
Histopathology
Radiology
Synthetic eugenol
Mohammad
Farajli Abbasi
mohammad.f.abbasi@vet.uk.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
LEAD_AUTHOR
Mohammad Mahdi
Molaei
molaei_mm@uk.ac.ir
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Reza
Kheirandish
3
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Ali
Mostafavi
amostafavi@uk.ac.ir
4
Department of Chemistry, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, Iran
AUTHOR
Dyce KM, Sack WO, Wensing CJG. Textbook of Veterinary Anatomy-E-Book. Edinburgh, UK: Elsevier Health Sciences 2009;355-360.
1
Al-Sobayil FA. A new simple device for dehorning in small ruminants. Small Rumin Res 2007; 67(2-3):232-234.
2
Oehme FW. Textbook of large animal surgery. Baltimore, USA: Williams and Wilkins 1988;180-185.
3
Fubini SL, Ducharme NG. Textbook of farm animal surgery: St. Louis, USA: Saunders; 2004;511-515.
4
Koger LM. Dehorning by injection of calcium chloride. Vet Med Small Anim Clin 1976; 71(6):824-825.
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Guidotti TL. Critique of available studies on the toxicology of kretek smoke and its constituents by routes of entry involving the respiratory tract. Arch Toxicol 1989; 63(1):7-12.
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Kihurani D, Mbiuki S, Ngatia T. Healing of dehorning wounds. Brit Vet J 1989; 145(6):580-585.
7
Petrie N, Mellor D, Stafford K, et al. Cortisol responses of calves to two methods of disbudding used with or without local anesthetic. New Zeal Vet J 1996; 44(1):9-14.
8
Bengtsson B, Menzel A, Holtenius P, et al. Cryosurgical dehorning of calves: a preliminary study. Vet Rec 1996; 138(10):234-237.
9
Molaei MM, Mostafavi A, Kheirandish R, et al. Study of disbudding goat kids following injection of clove oil essence in horn bud region. Vet Res Forum 2015; 6(1):17-22.
10
Pavithra B. Eugenol-A Review. Indian J Med Res. 1981; 73:443-451.
11
Carrasco A, Espinoza C, Cardile V, et al. Eugenol and its synthetic analogues inhibit cell growth of human cancer cells (Part I). J Brazil Chem Soc 2008;19(3):543-548.
12
Alma MH, Ertas M, Nitz S, et al. Chemical composition and content of essential oil from the bud of cultivated Turkish clove (Syzygium aromaticum L.). Bioresources 2007;2(2):265-269.
13
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14
Guan W, Li S, Yan R, et al. Comparison of essential oils of clove buds extracted with supercritical carbon dioxide and other three traditional extraction methods. Food Chem 2007; 101(4):1558-1564.
15
Hernández-Sánchez P, López-Miranda S, Lucas-Abellán C, et al. Complexation of eugenol (EG), as main component of clove oil and as pure compound, with β-and HP-β-CDs. Food Nut Sci 2012; 3(6):716-723.
16
Ohkubo T, Shibata M. The selective capsaicin antagonist capsazepine abolishes the antinociceptive action of eugenol and guaiacol. J Dent Res 1997; 76(4):848-851.
17
Jaganathan SK, Supriyanto E. Antiproliferative and molecular mechanism of eugenol-induced apoptosis in cancer cells. Molecules 2012; 17(6):6290-6304.
18
Pisano M, Pagnan G, Loi M, et al. Antiproliferative and pro-apoptotic activity of eugenol-related biphenyls on malignant melanoma cells. Mol Cancer 2007; 6(1):8. doi:10.1186/1476-4598-6-8.
19
Kim GC, Choi DS, Lim JS, et al. Caspases-dependent apoptosis in human melanoma cell by eugenol. Korean J Anat 2006; 39(3):245-253.
20
Pal D, Banerjee S, Mukherjee S, et al. Eugenol restricts DMBA croton oil induced skin carcinogenesis in mice: Downregulation of c-Myc and H-ras, and activation of p53 dependent apoptotic pathway. J Dermatol Sci 2010; 59(1):31-39.
21
Kurian R, Arulmozhi DK, Veeranjaneyulu A, et al. Effect of eugenol on animal models of nociception. Indian J Pharmacol 2006; 38(5):341-345.
22
Daniel AN, Sartoretto SM, Schmidt G, et al. Anti-inflammatory and antinociceptive activities A of eugenol essential oil in experimental animal models. Rev Bras Farmacogn 2009; 19(1B):212-217.
23
Raghavenra H, Diwakr B, Lokesh B, et al. Eugenol:The active principle from cloves inhibits 5-lipoxygenase activity and leukotriene-C4 in human PMNL cells. Prostaglandins Leukot Essent Fatty Acids 2006; 74(1):23-27.
24
Robbers JE, Tyler VE. Tyler's herbs of choice: The therapeutic use of phytomedicinals. Binghampton, USA: Haworth Press Inc 199;92-98.
25
Thompson K, Bateman R, Morris P. Cerebral infarction and meningoencephalitis following hot-iron disbudding of goat kids. New Zeal Vet J 2005; 53(5):368-370.
26
Wright H, Adams D, Trigo F. Meningoencephalitis after hot-iron disbudding of goat kids. Vet Med Small Anim Clin. 1983; 78:599-601.
27
Sanford SE. Meningoencephalitis caused by thermal disbudding in goat kids. Can Vet J 1989; 30(10):832.
28
Hull BL. Dehorning the adult goat. Vet Clin N Am Food A 1995; 11(1):183-185.
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Buttle H, Mowlem A, Mews A. Disbudding and dehorning of goats. In Pract 1986; 8(2):63-65.
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Hague Ba, Hooper R. Cosmetic dehorning in goats. Vet Surg 1997; 26(4):332-334.
31
Alvarez L, Gutierrez J. A first description of the physiological and behavioural responses to disbudding in goat kids. Anim Welfare 2010; 19(1):55-59.
32
Hendrickson DA, Baird AN. Text book of Turner and McIlwraith's techniques in large animal surgery. Baltimore, USA: John Wiley & Sons; 2013;293-296.
33
ORIGINAL_ARTICLE
Royal jelly protects male mice against nicotine-induced reproductive failure
This study evaluated the possible protective effect of royal jelly (RJ) on sperm parameters and sperm malondialdehyde (MDA) concentration and in vitro fertilizing potential in nicotine (NIC) exposed male mice. Thrtiy-six male BALB/c mice were randomly divided into six groups (n = 6). Group 1 received normal saline, group 2 received 100 mg kg-1 per day RJ, groups 3 and 4 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively and groups 5 and 6 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively plus RJ. Caudal epididymal sperm characteristics, lipid peroxidation and in vitro fertilizing capacity and embryo development were evaluated after 35 days. The NIC treatment caused a significant decrease in sperm motility and viability and fertilization rate along with poor blastocyst formation and increased sperm DNA damage and MDA levels. Moreover, the incidences of chromatin abnormality in spermatozoa were significantly higher in NIC-exposed mice than those of control. Nevertheless, RJ treatment improved sperm parameters and in vitro fertilization outcome as well as sperm lipid peroxidation level. Data from the current study suggest that RJ has a potential repro-protective action against NIC-induced sperm abnormalities and embryotoxicity in mice.
https://vrf.iranjournals.ir/article_32088_d9bcf6259819a151521c59b15fa9dd6c.pdf
2018-09-15
231
238
10.30466/vrf.2018.32088
malondialdehyde
Mice
Nicotine
Royal jelly
Sperm
Farnam
Azad
st_f.azad@urmia.ac.ir
1
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
AUTHOR
Vahid
Nejati
2
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
AUTHOR
Ali
Shalizar-Jalali
3
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Gholamreza
Najafi
g.najafi@mail.urmia.ac.ir
4
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Fatemeh
Rahmani
5
Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
AUTHOR
McLachlan RI, de Kretser DM. Male infertility: The case for continued research. Med J Aust 2001; 174(3): 116-117.
1
Oyeyipo IP, Raji Y, Emikpe BO, et al. Effects of nicotine on sperm characteristics and fertility profile in adult male rats: A possible role of cessation. J Reprod Infertil 2011; 12(3): 201-207.
2
Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: Possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxicol Sci 2010; 116(2): 647-659.
3
Nesseim W, Haroun H, Mostafa E, et al. Effect of nicotine on spermatogenesis in adult albino rats. Andrologia 2011; 43(6): 398-404.
4
Kavitharaj N, Vijayammal P. Nicotine administration induced changes in the gonadal functions in male rats. Pharmacology 1999; 58(1): 2-7.
5
Aydos K, Güven M, Can B, et al. Nicotine toxicity to the ultrastructure of the testis in rats. BJU Int 2001; 88(6): 622-626.
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Yildiz D, Ercal N, Armstrong DW. Nicotine enantiomers and oxidative stress. Toxicology 1998; 130(2):155-165.
7
Arabi M. Nicotinic infertility: Assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia 2004; 36(5): 305-310.
8
Han B, Li C, Zhang L, et al. Novel royal jelly proteins identified by gel-based and gel-free proteomics. J Agric Food Chem. 2011; 59(18):10346-10355.
9
Hattori N, Nomoto H, Fukumitsu H, et al. Royal jelly and its unique fatty acid, 10-hydroxy-trans-2-decenoic acid, promote neurogenesis by neural stem/progenitor cells in vitro. Biomed Res 2007; 28(5): 261-266.
10
Abdelhafiz AT, Muhamad JA. Midcycle pericoital intravaginal bee honey and royal jelly for male factor infertility. Int J Gynecol Obstet 2008; 101(2):146-149.
11
Edwards R, Steptoe P, Purdy J. Fertilization and cleavage in vitro of preovulator human oocytes. Nature 1970; 227(5265): 1307-1309.
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Shalizar Jalali A, Najafi G, Hosseinchi M, et al. Royal jelly alleviates sperm toxicity and improves in vitro fertilization outcome in Stanozolol-treated mice. Iran J Reprod Med 2015; 13(1):15-22.
14
Khosh AH, Hasanzadeh S, Shalizar Jalali A. Ameliorative effects of Achillea millefolium inflorescences alcoholic extract on nicotine-induced reproductive toxicity in male rat: Apoptotic and biochemical evidences. Vet Res Forum 2017; 8(2): 97-104.
15
Jin L, Xue HY, Jin LJ, et al. Antioxidant and pancreas-protective effect of aucubin on rats with streptozotocin-induced diabetes. Eur J Pharmacol 2008; 582(1): 162-167.
16
Wyrobek AJ, Gordon LA, Burkhart JG, et al. An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals: A report of the US environmental protection agency gene-tox program. Mutat Res 1983; 115(1): 1-72.
17
Jalali AS, Najafi G, Rahimzadeh P. Summer savory (Satureja hortensis) can reduce spermatotoxic effects of doxorubicin in rats. Caspian J Reprod Med 2015; 1(1): 2-7.
18
Aziz N, Said T, Paasch U, et al. The relationship between human sperm apoptosis, morphology and the sperm deformity index. Hum Reprod 2007; 22(5): 1413-1419.
19
Talebi AR, Ghasemzadeh J, Khalili MA, et al. Sperm chromatin quality and DNA integrity in partial versus total globozoospermia. Andrologia 2018;50(1). doi: 10.1111/and.12823. Epub 2017 May 18.
20
Kazerooni T, Asadi N, Jadid L. Evaluation of sperm's chromatin quality with acridin orange test, Chromomycine A3 and aniline blue staining in couple with recurrent abortion. Fertil Steril 2009; 92(3): S206.
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Erenpreiss J, Bars J, Lipatnikova V, et al. Comprative study of cytochemical tests for sperm chromatin integrity. J Androl 2001; 22; 45-53.
22
Janero DR. Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury. Free Radic Biol Med 1990; 9(6): 515-540.
23
Byers SL, Payson SJ, Taft RA. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology 2006; 65(9): 1716-1726.
24
González R, Ruiz-León Y, Gomendio M, et al. The effect of glucocorticoids on mouse oocyte in vitro maturation and subsequent fertilization and embryo development. Toxicol In Vitro 2010; 24(1): 108-115.
25
Wang X, Falcone T, Attaran M, et al. Vitamin C and vitamin E supplementation reduce oxidative stress- induced embryo toxicity and improve the blastocyst development rate. Fertil Steril 2002; 78(6): 1272-1277.
26
Silici S, Ekmekcioglu O, Eraslan G, et al. Antioxidative effect of royal jelly in cisplatin-induced testes damage. Urology 2009; 74(3): 545-551.
27
Guo H, Ekusa A, Iwai K, et al. Royal jelly peptides inhibit lipid peroxidation in vitro and in vivo. J Nutr Sci Vitaminol (Tokyo) 2008; 54(3): 191-195.
28
Elnagar SA. Royal jelly counteracts bucks’ “summer infertility”. Anim Reprod Sci 2010; 121(1): 174-180.
29
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30
Sankako MK, Garcia PC, Piffer RC, et al. Semen and reproductive parameters during some abstinence periods after cigarette smoke exposure in male rats. Braz Arch Biol Technol 2013; 56(1): 93-100.
31
Jorsaraei SGA, Shibahara H. The in-vitro effects of nicotine, cotinine and leptin on sperm parameters analyzed by CASA system. Iran J Reprod Med 2008; 6(3): 157-165.
32
Barrios F, Filipponi D, Pellegrini M, et al. Opposing effects of retinoic acid and FGF9 on Nanos2 expression and meiotic entry of mouse germ cells. J Cell Sci 2010; 123(6): 871-880.
33
Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev 1995; 7(4): 659-668.
34
Sikka SC. Andrology lab corner: Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J androl 2004; 25(1): 5-18.
35
Pacifici R, Altieri I, Gandini L, et al. Environmental tobacco-smoke-nicotine and cotinine concentration in semen. Environ Res 1995; 68(1):69-72.
36
Goss D, Oyeyipo IP, Skosana BT, et al. Ameliorative potentials of quercetin against cotinine-induced toxic effects on human spermatozoa. Asian Pac J Reprod 2016; 5(3):193-197.
37
Shen HM, Ong CN. Detection of oxidative DNA damage in human sperm and its association with sperm function and male infertility. Free Radic Biol Med 2000; 28(4): 529-536.
38
Moazamian R, Polhemus A, Connaughton H, et al. Oxidative stress and human spermatozoa: Diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation. Mol Hum Reprod 2015; 21(6): 502-515.
39
Saleh RA, Agarwal A, Sharma RK, et al. Effect of cigarette smoking on levels of seminal oxidative stress in infertile men: A prospective study. Fertil Steril 2002; 78(3): 491-499.
40
Schulte RT, Ohl DA, Sigman M, et al. Sperm DNA damage in male infertility: Etiologies, assays, and outcomes. J Assist Reprod Genet 2010; 27(1): 3-12.
41
La Maestra S, de Flora S, Micale RT. Effect of cigarette smoke on DNA damage, oxidative stress, and morphological alterations in mouse testis and spermatozoa. Int J Hyg Environ Health 2015; 218(1):117-122.
42
Sadeghi MR, Hodjat M, Lakpour N, et al. Effects of sperm chromatin integrity on fertilization rate and embryo quality following intracytoplasmic sperm injection. Avicenna J Med Biotechnol 2009; 1(3): 173-180.
43
Cho C, Jung-Ha H, Willis WD, et al. Protamine 2 deficiency leads to sperm DNA damage and embryo death in mice. Biol Reprod 2003; 69(1): 211-217.
44
Sharma R, Said T, Agarwal A. Sperm DNA damage and its clinical relevance in assessing reproductive outcome. Asian J Androl 2004; 6(2):139-148.
45
Yamamoto Y, Isoyama E, Sofikitis N, et al. Effects of smoking on testicular function and fertilizing potential in rats. Urol Res 1998; 26(1): 45-48.
46
Wang J, Wilcken DE, Wang XL. Cigarette smoke activates caspase-3 to induce apoptosis of human umbilical venous endothelial cells. Mol Genet Metab 2001; 72(1): 82-88.
47
Ghanbari E, Nejati V, Khazaei M. Antioxidant and protective effects of royal jelly on histopathological changes in testis of diabetic rats. Int J Reprod Biomed 2016; 14(8): 519-526.
48
Pourmoradian S, Mahdavi R, Mobasseri M, et al. Effects of royal jelly supplementation on glycemic control and oxidative stress factors in type 2 diabetic female: A randomized clinical trial. Chin J Integr Med 2014; 20(5): 347-352.
49
Shahzad Q, Mehmood MU, Khan H, et al. Royal jelly supplementation in semen extender enhances post-thaw quality and fertility of Nili-Ravi buffalo bull sperm. Anim Reprod Sci 2016; 167:83-88.
50
ORIGINAL_ARTICLE
Evaluation of intravitreal injection of pentoxifylline in experimental endotoxin-induced uveitis in rabbits
The objective of the present study was to investigate the clinical and histopathological effects of intravitreal injection of pentoxifylline (PTX) the management of an experimental model of uveitis. Fifty-two rabbits were divided randomly into six intravitreal treated groups as below: 1) Balanced salt solution (BSS), 2) Salmonella typhimurium lipopolysaccharide endotoxin (LPS) + BSS, 3) LPS + PTX 100 μg, 4) LPS + PTX 500 μg, 5) BSS + PTX 100 μg and 6) BSS + PTX 500 μg. Inflammation was evaluated by clinical examinations using slit lamp on days 1, 3, 5 and 7 post injections and histopathological examinations were also performed at the end of the study. Clinical examinations demonstrated a statistically significant difference between group 1 and group 2 on day 5 and day 7. Moreover, the comparison of clinical severity scores of group 1 with groups 3, 4, 5 and 6, on third, fifth and seventh post-injection days showed statistically significant differences. The mean histopathological inflammation intensity score in groups 5 and 6 was significantly higher than group 1. The mean histopathological inflammation intensity score in groups 3, 4, 5 and 6 was significantly higher than group 2. Intravitreal injection of PTX in an experimental model of uveitis in rabbits not only does not reduce inflammation but also leads to inflammation when used alone or in combination with LPS.
https://vrf.iranjournals.ir/article_32083_ade87ecb7370b86797af78821664df92.pdf
2018-09-15
239
244
10.30466/vrf.2018.32083
Endotoxin-induced uveitis
Intravitreal injection
Pentoxifylline
Tumor necrosis factor-α
Mohammad Reza
Khalili
1
Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Amin Hossein
Amini
a.h.amini@shirazu.ac.ir
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
Mohammad
Abbaszadeh Hasiri
abbaszadeh@shirazu.ac.ir
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Effat
Baghaei Moghaddam
4
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Masoomeh
Eghtedari
eghtedarim@gmail.com
5
Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Mohammad
Azizzadeh
m_azizzadeh@um.ac.ir
6
Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
AUTHOR
Mousa
Zare
7
Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Masood
Yasemi
masoodyasemi@yahoo.com
8
Poostchi Ophthalmology Research Center, Department of Ophthalmology, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Siddique SS, Shah R, Suelves AM, et al. Road to remission: A comprehensive review of therapy in uveitis. Expert Opin Investig Drugs 2011; 20(11): 1497-1515.
1
Durrani O, Tehrani N, Marr J, et al. Degree, duration, and causes of visual loss in uveitis. BrJ Ophthalmol 2004; 88(9): 1159-1162.
2
Franks WA, Limb GA, Stanford MR, et al. Cytokines in human intraocular inflammation. Curr Eye Res 1992; 11: 187-191.
3
de Boer JH, Van Haren MAC, de Vries-Knoppert W, et al. Analysis of IL-6 levels in human vitreous fluid obtained from uveitis patients, patients with proliferative intraocular disorders and eye bank eyes. Curr Eye Res 1992; 11: 181-186.
4
Hofman FM, Hinton DR. Tumor necrosis factor-alpha in the retina in acquired immune deficiency syndrome. Investig Ophthalmol Vis Sci 1992; 33(6): 1829-1835.
5
De Vos AF, Hoekzema R, Kijlstra A. Cytokines and uveitis, a review. Curr Eye Res 1992; 11(6): 581-597
6
Demir T, Gödekmerdan A, Balbaba M, et al. The effect of infliximab, cyclosporine A and recombinant IL-10 on vitreous cytokine levels in experimental autoimmune uveitis. Indian J Ophthalmol 2006; 54(4): 241-245.
7
Lindstedt EW, Baarsma GS, Kuijpers RW, et al. Anti-TNF-α therapy for sight threatening uveitis. Br J Ophthalmol 2005; 89(5): 533-536.
8
Yoshida M, Yoshimura N, Hangai M, et al. Interleukin-1 alpha, interleukin-1 beta, and tumor necrosis factor gene expression in endotoxin-induced uveitis. Invest Ophthalmol Vis Sci 1994; 35 (3): 1107-1113.
9
Salhiyyah K, Senanayake E, Abdel‐Hadi M, et al. Pentoxifylline for intermittent claudication. Cochrane Database Syst Rev 2012; 18:1:CD005262. doi: 10.1002/14651858.CD005262.pub2.
10
George J, Abel PW. Pentoxifylline. In: xPharm: The comprehensive pharmacology reference. New York, USA: Elsevier 2011; 1-18.
11
Salyer JL, Bohnsack J, Knape WA, et al. Mechanisms of tumor necrosis factor-alpha alteration of PMN adhesion and migration. Am J Pathol 1990; 136(4): 831-841.
12
Bessler H, Gilgal R, Djaldetti M, et al. Effect of pentoxifylline on the phagocytic activity, cAMP levels, and superoxide anion production by monocytes and polymorphonuclear cells. J Leukoc Biol 1986; 40(6): 747-754.
13
Leimenstoll G, Zabel P, Schroeder P, et al. Suppression of OKT3-induced tumor necrosis factor alpha formation by pentoxifylline in renal transplant recipients. Transplant Proc 1993; 25(1): 561-563.
14
Fong Y, Tracey KJ, Moldawer L, et al. Antibodies to cachectin/tumor necrosis factor reduce interleukin 1 beta and interleukin 6 appearance during lethal bacteremia. J Exp Med 1989; 170: 1627-1633.
15
Moreira A, Sampaio EP, Zmuidzinas A, et al. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J Exp Med 1993; 177: 1675-1680.
16
Neuner P, Klosner G, Schauer E, et al. Pentoxifylline in vivo down-regulates the release of IL-1 beta, IL-6, IL-8 and tumour necrosis factor-alpha by human peripheral blood mononuclear cells. Immunology 1994; 83(2): 262-267.
17
Appenzeller S, Hazel E. Pentoxifylline for the treatment of anterior uveitis in Behcet’s disease: Possible alternative for TNF blockers. Rheumatol Int 2011; 31(11): 1511-1513.
18
Yasui K, Ohta K, Kobayashi M, et al. Successful treatment of Behçet disease with pentoxifylline. Ann Intern Med 1996; 124(10): 891-893.
19
Avunduk AM, Avunduk MC, Oztekin E, et al. Characterization of T lymphocyte subtypes in endotoxin-induced uveitis and effect of pentoxifylline treatment. Curr Eye Res 2002; 24(2): 92-98.
20
Erşanli D, Karadayi K, Toyran S, et al. The efficacy of hyperbaric oxygen for the treatment of experimental uveitis induced in rabbits. Ocul Immunol Inflamm 2005; 13(5): 383-388.
21
Verma MJ, Mukaida N, Vollmer-Conna U, et al. Endotoxin-induced uveitis is partially inhibited by anti–IL-8 antibody treatment. Invest Ophthalmol Vis Sci 1999; 40: 2465-2470.
22
Kanai K, Ito Y, Nagai N, et al. Effects of instillation of eye drops containing disulfiram and hydroxypropyl-β-cyclodextrin inclusion complex on endotoxin-induced uveitis in rats. Curr Eye Res 2012; 37: 124-131.
23
Koizumi K, Poulaki V, Doehmen S, et al. Contribution of TNF-α to leukocyte adhesion, vascular leakage, and apoptotic cell death in endotoxin-induced uveitis in vivo. Invest Ophthalmol Vis Sci 2003; 44(5): 2184-2191.
24
Santos Lacomba M, Marcos Martin C, Gallardo Galera J, et al. Aqueous humor and serum tumor necrosis factor-α in clinical uveitis. Ophthalmic Res 2001; 33(5): 251-255.
25
De Vos AF, Van Haren MAC, Verhagen C, et al. Systemic anti-tumor necrosis factor antibody treatment exacerbates endotoxin-induced uveitis in the rat. Exp Eye Res 1995; 61(6): 667-675.
26
Van Furth A, Verhard‐Seijmonsbergen E, Van Furth R, et al. Effect of lisofylline and pentoxifylline on the bacterial‐stimulated production of TNF‐α, IL‐1β and IL‐10 by human leucocytes. Immunology 1997; 91(2): 193-196.
27
Chou PI, Chang JS, Chen JT, et al. The short-term effect of pentoxifylline on rabbit choroidal blood flow. J Ocul Pharmacol Ther 2000; 16(5): 455-462.
28
Schmetterer L, Kemmler D, Breiteneder H, et al. A randomized, placebo-controlled, double-blind cross-over study of the effect of pentoxifylline on ocular fundus pulsations. Am J Ophthalmol 1996; 121(2): 169-176.
29
Yasukawa T, Ogura Y, Sakurai E, et al. Intraocular sustained drug delivery using implantable polymeric devices. Adv Drug Deliv Rev 2005; 57(14): 2033-2046.
30
Sen HA, Campochiaro PA. Stimulation of cyclic adenosine monophosphate accumulation causes breakdown of the blood-retinal barrier. Invest Ophthalmol Vis Sci 1991; 32(7): 2006-2010.
31
Krause PJ, Kristie J, Wang W, et al. Pentoxifylline enhancement of defective neutrophil function and host defense in neonatal mice. Am J Pathol 1987; 129(2): 217-222.
32
London NJS, Garg SJ, Moorthy RS, et al. Drug-induced uveitis. J Ophthalmic Inflamm Infect 2013; 3: 43. doi.org/10.1186/1869-5760-3-43.
33
Cunningham Jr ET, Pasadhika S, Suhler EB, et al. Drug-induced inflammation in patients on TNFα inhibitors. Ocul Immunol Inflamm 2012; 20(1): 2-5.
34
Viguier M, Richette P, Bachelez H, et al. Paradoxical adverse effects of anti-TNF-alpha treatment: Onset or exacerbation of cutaneous disorders. Expert Rev Clin Immunol 2009; 5(4): 421-431.
35
ORIGINAL_ARTICLE
Effects of feeding with broiler litter in pellet-form diet on Qizil fattening lambs’ performance, nutrient digestibility, blood metabolites and husbandry economics
The aim of this study was to evaluate the feeding of Qizil fattening lambs with different levels of broiler litter (BL) on their weight gain, dry matter intake (DMI), nutrient digestibility, selected blood metabolites and husbandry economics. During an eight-weeks experimental period, 28 male lambs (an average of 42.21 ± 5.63 kg and ages of 7-8 months) were allocated randomly to one of four dietary treatments, including; control diet with no litter (NL, n = 7), diet containing 5% BL (LL, n = 7), diet with 10% BL (ML, n = 7) and diet containing 15.00% BL (HL, n = 7) as dry matter (DM) basis. The lambs were kept in individual pens and had free access to feed and water (ad libitum) throughout the study. In this research, DMI and feed conversion ratio were not influenced significantly by the dietary treatments, while average daily gain (ADG) was found to be significant. The lambs which consumed LL diet (5.00% BL) had the greatest ADG. The DM digestibility significantly influenced by dietary treatments, as the HL diet (15.00% BL) had the least DM digestibility. There were also significant differences in the crude protein and acid detergent fiber digestibility among the treatments. Blood urea nitrogen and glucose levels were significantly affected by the treatments. Low litter treatment had the least FCR and the highest economical advantage. In conclusion, feeding male Qizil lambs with BL can reduce production cost without any negative effects on performance.
https://vrf.iranjournals.ir/article_32081_e046d53c0f928eeac273bb6d843e92cd.pdf
2018-09-15
245
251
10.30466/vrf.2018.32081
Broiler litter
Economics
Growth performance
Qizil breed
Mohammad Reza
Rahimi
1
Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
Younes
Alijoo
y.alijoo@urmia.ac.ir
2
Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
LEAD_AUTHOR
Rasoul
Pirmohammadi
r.pirmohammadi@urmia.ac.ir
3
Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
Masoud
Alimirzaei
m.mirzayi1362@yahoo.com
4
Department of Animal Sciences, Faculty of Agriculture, Urmia University, Urmia, Iran
AUTHOR
El-Sabban FF, Bratzler Jw, long TA, et al. Value of processed poultry waste as a feed for ruminants. J Anim Sci 1970; 31: 107-111.
1
Nadeem MA, Ali A, Azim A, et al. Effect of feeding broiler litter on growth and nutrient utilization by Barbari goat. Asian-Australas J Anim Sci 1993; 6:73-77.
2
Boda K. Non-conventional Feedstuffs in the nutrition of farm animals. New York, USA: Elsevier 1999; 13-15.
3
Eliasi G. Evaluating beta- lactoglobulin gene polymorphism in the five sheep breeds through PCR-RELP. MSc Thesis. Agricultural Faculty of Tabriz University, Tabriz: 2002.
4
Daniel J, Olson KC. Feeding poultry litter to beef cattle, MU Guide. MU Extension, University of Missouri-Columbia, 2005: G2077.
5
Goetsch AL, Aiken GE. Broiler litter in ruminant diets implications for use as a low-cost by-product feedstuff for goats. In: Merkel RC, Adebe G, Goetach AL (Eds). The opportunity and challenged of enhancing goat production in East Africa. Langston, OK: Langston University Press 2000; 58-69.
6
Fontenot JP, Hancock JW. Utilization of poultry litter as feed for beef cattle. Department of Animal and Poultry Sciences Virginia Polytechnic Institute and State University Blacksburg, Virginia, USA: 2001.
7
Areghore EM. Chemical composition and nutritive value of some tropical by products feedstuffs for small ruminant’s in vivo and in vitro digestibility. Anim Feed Sci Technol 2000; 85:99-109.
8
Jordon DJ, Klopfenstein TJ, Adams DC. Dried poultry waste for cows grazing low-quality winter forage. J Anim Sci 2002; 80(3):818-824.
9
Animut G, Merkel RC, Abede G, et al. Effects of level of broiler litter in diets containing wheat straw on performance of Alpine doelings. Small Ruminant Res 2002; 44: 125-142.
10
NRC. Nutrient requirements of small ruminants: Sheep, goats, cervide, and new world camelids. National Academy of Science. Washington, USA. 2007;1-384.
11
Johansson MS. Chewing behaviour of growing cattle. Swedish University of Agricultural Sciences. Department of Animal Environment and Health Section of Production Systems. Skara, Sweden; 2011. Student report 279.
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Church DC. Taste, appetite and regulation of energy balance and control of food intake. I. Appetite, taste and palatability. In: Church DC (Ed). Digestive physiology and nutrition of ruminants. Oregon, USA: Oxford Press 1979; 281-290.
13
Pond WG, Church DC, Pond KR, et al. Basic animal nutrition and feeding. 5th ed. New York, USA: Wiley 2005; 23-25.
14
AOAC: Official methods of analysis. 15th ed. Washington, USA: Association of Official Analytical Chemists 1990.
15
Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J Dairy Sci 1991; 74(10):3583-3597.
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Van Keulen J, Young BA. Evaluation of acid-insoluble ash as a natural marker in ruminant digestibility studies. J Anim Sci 1977; 44, 282-287.
17
Obeidat BS, Awawdeh MS, Abdullah AY, et al. Effect of feeding broiler litter on performance of Awassi lambs fed finishing diets. Anim. Feed Sci Technol 2011; 165, 15-22.
18
Knowlton KF, Wilkerson VA, Casper DP, et al. Manure nutrient excretion by Jersey and Holstein cows. J Dairy Sci 2010;93(1):407-412.
19
Fontenot JP. Feeding poultry wastes to cattle. Department of Animal and Poultry Sciences Virginia Polytechnic Institute and State University Blacksburg, Virginia, USA: 1996; 38.
20
Mavimbela DT, van Ryssena JBJ, Last R. The effect of high broiler litter diets as survival ration on the health of sheep. J S Afr Vet Assoc 1997; 68 (4):121-124.
21
Cullison AE. Use of poultry manures in steer finishing rations. J Anim Sci 1976; 42(1):219-228.
22
Elemam MB, Fadelelseed AM, Salih AM. Growth performance, digestibility, N-balance, and rumen fermentation of lambs fed different levels of deep-stack broiler litter. Res J Anim Vet Sci 2009; 4: 9-16.
23
Talib NH, Ahmed FA. Digestibility, degradability and dry matter intake of deep-stacked poultry litter by sheep and goats. J Anim Vet Adv 2008; 7(11): 1474-1479.
24
Negesse T, Patra AK, Dawson LJ, et al. Performance of Spanish and Boer × Spanish doelings consuming diets with different levels of broiler litter. Small Rumin 2007; 69:187-197.
25
Van Soest PJ, Mason VC. The influence of the Maillard reaction upon the nutritive value of fibrous feeds. Anim Feed Sci Technol 1991; 32(1-3): 45-53.
26
Chaudhry SM, Naseer Z. Silages of citrus pulp-poultry litter-corn forage for sheep. Pak J Agri Sci 2006; 43: 173-179.
27
Corrêa DS, Magalhães RT, Siqueira DCB. Ruminal dry matter and fiber fraction degradability from two stylos cultivars. Arq Bras Med Vet Zootec 2014; 66: 1155-1162.
28
Mertens DR. Creating a system for meeting the fiber requirements of dairy cows. J Dairy Sci 1997; 80:1463-1481.
29
National Research Council (NRC). Nutrient requirements of dairy cattle. Washington DC, USA: The National Academies Press 2001; 72-74.
30
D’Mello JPF. Amino acids in animal nutrition. Edinburgh, UK: CABI Publishing 2003; 265-291.
31
Paul BN, Gubta BS, Srivastava JP. Influence of feeding unconventional cakes and poultry manure mixture on growth and feed efficiency in crossbred calves. Ind J Anim Nutr 1993; 10: 169-171.
32
ORIGINAL_ARTICLE
Goat paratuberculosis in Shiraz: Histopathological and molecular approaches
In the present study, Mycobacterium avium subsp. paratuberculosis (MAP) was investigated in goats slaughtered in Shiraz abattoir using histopathological examinations and polymerase chain reaction (PCR). Ilium and mesenteric lymph node samples from 66 suspected goat carcasses to Johne’s disease were collected. Among 66 examined slaughtered goats, nine (13.63%) goats were positive for MAP in both histopathological and PCR examinations. Eight goats were positive in PCR method while no lesion related to Johne’s disease was observed in their histopathological sections. All positive goats in histopathological examination were also positive in PCR. Based on the results of PCR, the detection rate of MAP in Shiraz abattoir was 25.80% (17 goats). According to the present findings, although both histopathological and PCR methods are appropriate for detecting Johne’s disease, PCR is more sensitive than histopathological examination.
https://vrf.iranjournals.ir/article_32080_06bc401eb4841ad87197722a6eddbf82.pdf
2018-09-15
253
257
10.30466/vrf.2018.32080
Goat
Histopathology
Johne’s disease
Mycobacterium avium subsp. paratuberculosis
PCR
Abdollah
Derakhshandeh
drkhshnd77@gmail.com
1
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Fatemeh
Namazi
fnamazi@shirazu.ac.ir
2
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
LEAD_AUTHOR
Elmira
Khatamsaz
ekhatamsaz@yahoo.com
3
DVM Graduate, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Vida
Eraghi
v.eraghi@gmail.com
4
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Zahra
Hemati
z.hemati28@gmail.com
5
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
AUTHOR
Haghkhah M, Ansari-Lari M, Novin-Baheran A, et al. Herd-level prevalence of Mycobacterium avium paratuberculosis by bulk-tank milk PCR in Fars province (southern Iran) dairy herds. Prev Vet Med 2008; 86:8-13.
1
Sechi LA, Dow CT. Mycobacterium avium ss. paratuberculosis zoonosis – The hundred year war – beyond Crohn’s disease. Front Immunol 2015; 6:1-8.
2
Pithua P, Kollias NS. Estimated prevalence of caprine paratuberculosis in boer goat herds in Missouri, USA. Vet Med Int 2012; 674085. doi:10.1155/2012/ 674085.
3
Fawzy A, Prince A, Hassan AA, et al. Epidemiological studies on Johne’s disease in ruminants and Crohn’s disease in humans in Egypt. Int J Vet Sci Med 2013; 1:79-86.
4
Erume J, Spergser J, Rosengarten R. Rapid detection of Mycobacterium avium subsp. paratuberculosis from cattle and zoo animals by nested PCR. Afr Health Sci 2001; 1(2):83-89.
5
Tiwari A, VanLeeuwen JA, McKenna SLB, et al. Johne’s disease in Canada Part I: Clinical symptoms, pathophysiology, diagnosis, and prevalence in dairy herds. Can Vet J 2006; 47(9):874-882.
6
Stabel JR. Johne’s disease: a hidden threat. J Dairy Sci 1998; 81:283-288.
7
Whitlock RH, Wells SJ, Sweeney RW, et al. ELISA and fecal culture for paratuberculosis (Johne’s disease): Sensitivity and specificity of each method. Vet Microbiol 2000; 77:387-398.
8
Behr MA, Collins DM. Paratuberculosis organism, disease, control. Wallingford, UK: CABI Publishing 2010;169-179.
9
Delgado F, Aguilar D, Garbaccio S, et al. Detection of Mycobacterium avium subsp. paratuberculosis by a direct in situ PCR method. Vet Med Int 2011; 2011: 267102. doi: 10.4061/2011/267102.
10
Slana I, Kralik P, Kralova A, et al. On-farm spread of Mycobacterium avium subsp. paratuberculosis in raw milk studied by IS900 and F57 competitive real time quantitative PCR and culture examination. Int J Food Microbiol 2008; 128:250-257.
11
Green EP, Tizard ML, Moss MT, et al. Sequence and characteristics of IS900, an insertion element identified in human Crohne’s disease isolate of Mycobacterium paratuberculosis. Nucleic Acids Res 1989;
12
17:9063-9073.
13
Bull TJ, Hermon-Taylor J, Pavlik I, et al. Characterization of IS900 loci in Mycobacterium avium subsp. paratuberculosis and development of multiplex PCR typing. Microbiol 2000; 146:2185-2197.
14
Bancroft JD, Stevens A. Theory and practice of histological techniques. London, UK: Churchill Livingston1990; 21-119.
15
Prophet EB, Mills B, Arrington JB, Sobin LH. Laboratory methods in histotechnology. Washington DC, USA: American Registry of Pathology 1994; 219-222.
16
Sambrook J, Russell RW. Molecular cloning: A laboratory manual. 3rd ed. New York, USA: Cold spring harbor laboratory press 2001;47-53.
17
Corti S, Stephan R. Detection of Mycobacterium avium subspecies paratuberculosis specific IS900 insertion sequences in bulk-tank milk samples obtained from different regions throughout Switzerland. BMC Microbiol 2002; 2(1): 15. doi: 10.1186/1471-2180-2-15.
18
Haji Hajikolaei MR, Ghorbanpoor M, Solaymani M. The prevalence of Mycobacterium paratuberculosis infection in ileocecal valve of cattle slaughtered in Ahvaz abattoir, southern Iran. Iran J Vet Res2006; 7:2-15.
19
Djonnea B, Jensena MR, Grantb IR, et al. Detection by immunomagnetic PCR of Mycobacterium avium subsp paratuberculosis in milk from dairy goats in Norway. Vet Microbiol 2002; 92:135-143.
20
Kruze J, Salgado M, Paredes E, et al. Goat paratuberculosis in Chile: First isolation and confirmation of Mycobacterium avium subspecies paratuberculosis infection in a dairy goat. J Vet Diagn Invest 2006; 18:476-479.
21
Corpa JM, Garrido J, García- Marín JF, e al. Classification of lesions observed in natural cases of paratuberculosis in goats. J Comp Pathol 2000; 122:55-65.
22
Kheirandish R, Khodakaram Tafti A, Hosseini A. Classification of lesions and comparison of immunohistochemical and acid fast staining in diagnosis of naturally occurring paratuberculosis in goats. Small Ruminant Res 2009; 87:81-85.
23
ORIGINAL_ARTICLE
Distribution of Nosema Spp. in climatic regions of Iran
Nosemosis is one of the most prevalent bee diseases in the world causing significant economic losses in the global bee-keeping industry. This cross-sectional study was conducted during April-September, 2016 to investigate the prevalence of nosemosis in different climatic regions of Iran. A total of 183 apiaries were selected based on cluster sampling and the climate of apiaries under study was classified using Domarten method. In each apiary, five percent of the colonies were randomly sampled. A total of 183 adult bee samples were taken and examined by microscopic and polymerase chain reaction (PCR) methods for the presence of Nosema infections. According to the results, infection caused by Nosema ceranae was observed in all regions under study. The prevalence of N. ceranae was 46.40% (42.70–50.10). However, infection with Nosema apis was not observed in the samples in either pure form or as associated infection. Based on the results of PCR, the prevalenceof N. ceranae was 53.80% (46.60– 61.00) in humid, 71.00% (53.70–77.50) in semi-humid, 68.10% (61.40–74.80) in very humid, 29.40% (22.70–36.10) in arid, 34.30% (27.40–41.20) in semi-arid and 24.00% (17.90–30.00) in Mediterranean climates. The prevalence of infection in different climatic zones of the country was found to have significant differences (p < 0.001). According to the findings, N. ceranae was the only Nosema species in honeybees with a broad geographical dispersion in Iran. It seems that climate can influence the prevalence of mentioned parasite.
https://vrf.iranjournals.ir/article_32082_f26d66930b9e8141862e94c8f43eac6b.pdf
2018-09-15
259
263
10.30466/vrf.2018.32082
Climate
Honeybee
Iran
Nosemosis
Prevalence
Baharak
Mohammadian
bk.mohammadian@gmail.com
1
Department of Animal Science Research, Kurdistan Agricultural and Natural Resources Research and Education Center, (AREEO), Sanandaj, Iran
AUTHOR
Saied
Bokaie
sbokaie@ut.ac.ir
2
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
LEAD_AUTHOR
Mojtaba
Moharrami
moharrami@yahoo.com
3
Department of Honeybee, Silk Worm and wildlife Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
AUTHOR
Sedighe
Nabian
4
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Mohammad
Forsi
mforsi@gmail.com
5
Directorate of Health and Control of Bird, Honeybee and Silk Worm, Veterinary Organization, Tehran, Iran
AUTHOR
Webster T, Pomper K, Hunt G, et al. Nosema apis infection in worker and queen Apis mellifera. Apidologie 2004; 35(1): 49-54.
1
Fries I. Nosema apis - a parasite in the honeybee colony. Bee World 1993; 74: 5-19.
2
Oranlar E, Lotfi A, Aghdam Shahryar H. Seasonal incidence of some economic bee diseases (varroosis, nosemosis and American foulbrood) in honeybee colonies of northwestern Iran. Uludag Bee J 2011; 11: 25.
3
Paxton R., Klee J, Korpela S, et al. Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 2007; 38: 558-565.
4
Chen YP, Evans JD, Murphy C, et al. Morphological, molecular and phylogenetic characterization of Nosema ceranae, a microsporidian parasite isolated from the European honey bee Apis mellifera. J Eukar Microbiol 2009; 56: 142-147.
5
Weiss LM, Vossbrinck CR. Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the microsporidia. In: Wittner M, Weiss LM (Eds). The microsporidia and microsporidiosis. Washington DC, USA: American society for microbiology 1999; 129-171.
6
Martín-Hernández R, Meana A, Prieto L, et al. Outcome of colonization of Apis mellifera by Nosema ceranae. Appl Environ Microbiol 2007; 73(20): 6331-6338.
7
Higes M, Martín-Hernández R, Meana A. Nosema ceranae in Europe: An emergent type C Nosemosis. Apidologie 2010; 41: 375-392.
8
Klee J, Besana AM, Genersch E, et al. Widespread dispersal of the microsporidian Nosema ceranae, an emergent pathogen of the western honey bee, Apis mellifera. J Invertebr Pathol 2007; 96(1): 1-10.
9
Higes M, Martín-Hernández R, Meana A. Nosema ceranae, a new microsporidian parasite in honeybees in Europe. J Invertebr Pathol 2006; 92: 81-83.
10
Higes M, Martin-Hernandez R, Garrido-Bailon E, et al. Honeybee colony collapse due to Nosema ceranae in professional apiaries. Environ Microbiol Rep 2009; 1: 110-113.
11
Nabian S, Ahmadi K, Shirazi MN, et al. First detection of Nosema ceranae, a microsporidian protozoa of European honeybees (Apis mellifera) in Iran. Iran J Parasitol 2011; 6(3): 89-95.
12
Razmaraii N, Sadegh-Eteghad S, Babaei H, et al. Molecular identification of Nosema species in East Azerbaijan province, Iran. Arch Razi Inst 2013; 68(1): 23-27.
13
Modirrousta H, Moharrami M, Mansouri MA. Retrospective study of the Nosema ceranae infection of honey bee colonies in Iran (2004-2013). Arch Razi Inst 2014; 69(2): 197-200.
14
Aroee F, Azizi H, Shiran B, et al. Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran). Asian Pac J Trop Biomed 2017; 7(1): 10-13.
15
Kamyabi S, Farahani S. Effective climatic factors in mountainous regions of Iran for urban planning: A case study in Rudbar Qasran in the central Alborz. e-Bangi: J Soc Sci Humanit 2011; 6(1): 17-28.
16
Office international des epizooties, manual of diagnostic tests and vaccines for terrestrial animals. 2008; Chap. 2.2.4., Nosemosis of honey bees. http:// www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.02.04_nosemosis.pdf. Accessed June 25, 2017.
17
Fries I. Nosema ceranae in European honey bees (Apis mellifera). J Invertebr Pathol 2010; 103(S1): S73-S79.
18
Lotfi A, Jamshidi R, Aghdam Shahryar H, et al. The prevalence of nosemosis in honey bee colonies in Arasbaran region (northwestern Iran). American-Eurasian J Agric Environ Sci 2009; 5(2): 255-257.
19
Razmaraii N, Karimi H. A survey of nosema disease of honey bee (Apis mellifera) in East Azarbaijan province of Iran. J Anim Vet Adv 2010; 9(5): 879-882.
20
Davoudi J, Naderi A, Mohammadpour F, et al. Study of infection rate of suburb bee hives to parasites Nosema apis, Varroa spp. and Acarapis woodi in Miyaneh, Iran. J New Agric Sc 2009; 4(13): 39-43.
21
Tavassoli M, Eiganinejad S, Alizadeh Asl S. A survey on Nosema apis infection in apiaries of Urmia, north-west of Iran. Iran J Vet Sci Technol 2009; 1(1): 35
22
Moshaverinia A, Abedi V, Safaei H. A survey of Nosema apis infection in apiaries of North Khorasan province, Iran. Iran J Vet Sci Technol 2012; 4(2): 25-30.
23
Oǧuz B, Karapinar Z, Dinerç E, et al. Molecular detection of Nosema spp. and black queen-cell virus in honeybees in Van province, Turkey. Turk J Vet Anim Sci 2017; 41: 221-227.
24
Gajger IT, Vugrek O, Grilec D, et al. Prevalence and distribution of Nosema ceranae in Croatian honeybee colonies. Vet Med 2010; 55: 457-462.
25
Chen YW, Chung WP, Wang CH, et al. Nosema ceranae infection intensity highly correlates with temperature. J Invertebr Pathol 2012; 111; 264-267.
26
Invernizzia C, Abuda C, Tomascoa I, et al. Presence of Nosema ceranae in honeybees (Apismellifera) in Uruguay. J Invertebr Pathol 2009; 101: 150-153.
27
Chen Y, Evans JD, Smith IB, et al. Nosema ceranae is a long-present and wide-spread microsporidean infection of the European honey bee (Apis mellifera) in the United States. J Invertebr Pathol 2008; 97: 186-188.
28
Özgör E, Güzerin E, Keskin N. Determination and comparison of Nosema apis and Nosema ceranae in terms of geographic and climatic factors. Hacettepe J Biol Chem 2015; 43(1): 9-15.
29
de La Rocque S, Rioux JA, Slingenbergh J. Climate change: Effects on animal disease systems and implications for surveillance and control. Rev Sci Tech Off Int Epizoot 2008; 27(2): 339-354.
30
Fenoy S, Rueda C, Higes M, et al. High-level resistance of Nosema ceranae, a parasite of the honeybee, to temperature and desiccation. Appl Environ Microbiol 2009; 75: 6886-6889.
31
Stevanovic J, Simeunovic P, Gajic B, et al. Characteristics of Nosema ceranae infection in Serbian honey bee colonies. Apidologie 2013; 44: 522-536.
32
Cepero A, Martín-Hernández R, Bartolomé C, et al. Passive laboratory surveillance in Spain: Pathogens as risk factors for honey bee colony collapse. J Apic Res 2015; 54: 525-531.
33
Cornman RS, Chen YP, Schatz MC, et al. Genomic analyses of the microsporidian Nosema ceranae, an emergent pathogen of honey bees. PLoS Pathogens 2009; 5(6): 1-14.
34
Cox-Foster DL, Conlan S, Holmes EC, et al. A metagenomic survey of microbes in honey bee colony collapse disorder. Science 2007; 318: 283-287.
35
Higes M, Meana A, Bartolomé C, et al. Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen. Environ Microbiol Rep 2013; 5: 17-29.
36
ORIGINAL_ARTICLE
Ameliorative effect of Allium sativum extract on busulfan-induced oxidative stress in mice sperm
Busulfan is known to cause several adverse effects including reproductive toxicity in humans. Garlic (Allium sativum), a widely distributed medicinal plant, is highly regarded for its medicinal activities including antioxidant property.This study was conducted to assess whether garlic extract could serve as protective agents against testicular toxicity during busulfan treatment in a mice model.Seventy-two adult male mice were randomly divided into nine groups. In groups 1,2 and 3, distilled water, busulfan, and dimethyl sulfoxide and in the treatment groups hydro-alcoholic extract of garlic was administered orally at different doses per day (groups 4, 5 and 6; 200, 400, 800 mg kg-1 respectively). Groups 7, 8 and 9 were treated with the extract (200, 400 and 800 mg kg-1, respectively) plus busulfan. Following euthanasia, blood samples and epididymal sperm were collected.The busulfan-treated group showed significant decreases in sperm qualityparameters, and serum levels of testosterone, LH and FSH was observed in the busulfan-treated mice. In addition, the TAC levels and antioxidant enzymes activities were reduced and malondialdehyde (MDA) levels were increased in the busulfan-treated mice. Notably, garlic extract co-administration caused a considerable recovery in sperm qualityparameters, TAC levels, antioxidant enzymes activities, hormonal changes and MDA level. Based on our results, garlic has antioxidant effects against busulfan-induced testicular damages in mice.
https://vrf.iranjournals.ir/article_32079_414eb23fd3835b39617a203527a1aa55.pdf
2018-09-15
265
271
10.30466/vrf.2018.32079
Allium sativum
Busulfan
Mice
Reproduction
Spermatozoa
Ali
Soleimanzadeh
a.soleimanzadeh@urmia.ac.ir
1
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
LEAD_AUTHOR
Leila
Mohammadnejad
2
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Abbas
Ahmadi
3
Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
AUTHOR
Suriapraba E, Mani PR, Kumar RA, et al. Protective effect of NAC on busulfan induced clastogenesis in human peripheral blood lymphocytes. Int J Inst Pharm Life Sci 2012; 2(1): 172-177.
1
Sweetman SC. Martindale. London, UK: Pharmaceutical Press 2002; 33.
2
McClive PJ, Sinclair AH. Type II and type IX collagen transcript isoforms are expressed during mouse testis development. Biol Reprod 2003; 68(5): 1742-1747.
3
Kanatsu-Shinohara M, Toyokuni S, Morimoto T, et al. Functional assessment of self-renewal activity of male germline stem cells following cytotoxic damage and serial transplantation. Biol Reprod 2003; 68: 1801-1807.
4
Probin V, Wang Y, Zhou D. Busulfan-induced senescence is dependent on ROS production upstream of the MAPK pathway. Free Radic Biol Med 2007; 42(12):1858-1865.
5
Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Nutrition 2002; 18(10): 872-879.
6
Maymon BB, Yogev L, Marks A, et al. Sertoli cell inactivation by cytotoxic damage to the human testis after cancer chemotherapy. Fertil steril 2004; 81(5): 1391-1394.
7
Valko M, Leibfritz D, Moncol J, et al. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39(1): 44-84.
8
Saxena R, Garg P, Jain DK. In vitro anti-oxidant effect of vitamin E on oxidative stress induced due to pesticides in rat erythrocytes. Toxicol Int 2011; 18(1): 73-76.
9
Bozin B, Mimica-Dukic N, Samojlik I, et al. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem 2008; 111(4): 925-929.
10
Hammami I, Nahdi A, Mauduit C, et al. The inhibitory effects on adult male reproductive functions of crude garlic (Allium sativum) feeding. Asian J Androl 2008; 10(4): 593–601.
11
Abid-Essefi S, Zaied C, Bouaziz C, et al. Protective effect of aqueous extract of Allium sativum against zearalenone toxicity mediated by oxidative stress. Exp Toxicol Pathol 2012; 64(7): 689-695.
12
Song K, Milner JA. The influence of heating on the anticancer properties of garlic. J Nutr 2001; 131(3s): 1054S-1057S.
13
Santos J, Almajano MP, Carbo R. Antimicrobial, and antioxidants activity of crude onion (Allium cepa L.) extracts. Int J Food Sci Technol 2010; 45(2): 403-409.
14
Hosseini N. Khaki A. Effect of aqueous extract of garlic (Allium sativum) on sperms morphology, motility, concentration and its antioxidant activity in rats. Afinidad 2014; 80(566): 201-204.
15
Ghalehkandi JG. Garlic (Allium sativum) juice protects from semen oxidative stress in male rats exposed to chromium chloride. Anim Reprod 2014; 11(4): 526-532.
16
Erdemoglu N, Kupeli E, Yesilada E. Anti-inflammatory and anti-nociceptive activity assessment of plants used as remedy in Turkish folk medicine. J Ethnopharmacol 2003; 89(1): 123-129.
17
Homafar MA, Soleimanirad J, Ghanbari AA. A morphologic and morphometric study of adult mouse testis following different doses of busulfan administration. J Reprod Infertil 2006; 7(1):25-36.
18
Ahmadi A, Bamohabat Chafjiri S, Sadrkhanlou R. Effect of Satureja khuzestanica essential oil against fertility disorders induced by busulfan in female mice. Vet Res Forum 2017; 8(4): 281-286.
19
Mirfardi M, Johari H. The effect of hydro-alcoholic Allium sativum extracts on sexual hormones in mature male rats under chemotherapy with cyclo-phosphamide. Zahedan J Res Med Sci 2015; 15: 29-33.
20
Zobeiri F, Sadrkhanlou RA, Salami S, et al. The effect of ciprofloxacin on sperm DNA damage, fertility potential and early embryonic development in NMRI mice. Vet Res Forum 2012; 3: 131-135.
21
World Health Organization. WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction. 3rd ed. Cambridge, UK: Cambridge University Press 1992; 45-100.
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Wyrobek AJ, Gordon LA, Burkhart JG, et al. An evaluation of the mouse sperm morphology test and other sperm tests in nonhuman mammals. Mutat Res 1983; 115: 1-72.
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Narayana K, D'Souza UJ, Seetharama Rao KP. Ribvirin induced sperm shape abnormalities in Wistar rat. Mutal Res 2002; 513(1): 193-196.
24
Bungum M, Humaidan P, Spano M, et al. Predictive value of sperm chromatin structure assay (SCSA) Para-meters for the outcome of intrauterine insemination, IVF and ICSI. Hum Reprod 2004; 19(6): 1401-1408.
25
Hodjat M, Akhondi MA, Amirjanati N, et al. The comparison of four different sperm chromatin assays and their correlation with semen parameters. Tehran Uni Med J 2008; 65(Supl3): 33-40.
26
Meistrich ML, Brock WA, Grimes SR, et al. Nuclear protein transitions during spermatogenesis. Fed Porc 1978; 37(11): 2522-2525.
27
Talebi AR, Sarcheshmeh AA, Khalili MA, et al. Effect of ethanol consumption on chromatin condensation and DNA integrity of epididymal spermatozoa in rat. Alcohol 2011; 45(4): 403-409.
28
Deiss WP. Hormone assays and their clinical application. J Am Med Assoc 1967; 200(2):184-185.
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Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999; 299: 15-27.
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Rajesh MG, Latha MS. Protective activity of Glycyrrhiza glabra Linn. on carbon tetrachloride-induced peroxidative damage. Indian J Pharmacol 2004; 36(5): 284-287.
34
Hosseinzadeh H, Sadeghnia HR. Safranal, a constituent of Crocus sativus (saffron), attenuated cerebral ischemia induced oxidative damage in rat hippocampus. J Pharm Pharm Sci 2005; 8(3): 394-399.
35
Mohammad-Ghasemi F, Soleimanirad J, Ghanbari AA. An ultrastructural study on the apoptotic features of spermatogenic cells following busulfan treatment in adult mice. Reprod Infertil 2008; 8(4): 319-329.
36
Nasimi P, Tabandeh MR, Vahdati A, et al. Busulfan induces oxidative stress-and Bcl-2 family gene-related apoptosis in epididymal sperm and testis of adult male mice. Physiol Pharmacol 2015; 19(3): 208-215.
37
Dehghani F, Hassanpour A, Poost-Pasand A, et al. Protective effects of L-carnitine and homogenized testis tissue on the testis and sperm parameters of busulfan-induced infertile male rats. Iran J Reprod Med 2013; 11(9): 693-704.
38
Ghosh D, Das UB, Ghosh S, et al. Testicular gametogenic and steroidogenic activities in cyclophosphamide treated rat: A correlative study with testicular oxidative stress. Drug Chem Toxicol 2002; 25: 281-292.
39
Bordbar H, Esmaeilpour T, Dehghani F, et al. Stereological study of the effect of ginger's alcoholic extract on the testis in busulfan-induced infertility in rats. Iran J Reprod Med 2013; 11(6): 467-472.
40
Gerl A, Mühlbayer D, Hansmann G, et al. The impact of chemotherapy on Leydig cell function in long term survivors of germ cell tumors. Cancer 2001; 91(7): 1297-1303.
41
Ilbey YO, Ozbek E, Simsek A, et al. Potential chemoprotective effect of melatonin in cyclophosphamide-and cisplatin-induced testicular damage in rats. Fertil Steril 2009; 92(3): 1124-1132.
42
Banerjee SK, Mukherjee PK, Maulik SK. Garlic as an antioxidant: The good, the bad and the ugly. Phytother Res 2003; 17(2): 97-106.
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Charles DJ. Antioxidant properties of spices, herbs and other sources. New York, USA: Springer Science & Business Media 2012; 612-613.
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Ruwanpura SM, McLachlan RI, Meachem SJ. Hormonal regulation of male germ cell development. J Endocrinol 2010; 205(2): 117-131.
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Hajiuon B, Elahizadeh H. Effects of garlic (Allium sativum L.) hydro alcoholic extract on estrogen, progesterone and testosterone levels in rats exposed to cell phone radiation. Armaghane Danesh 2014; 19(5): 390-400.
46
Zarei L, Shahrooz R, Sadrkhanlou R, et al. Protective effects of cornus mas extract on in vitro fertilization potential in methotrexate treated male mice. Vet Res Forum 2015; 6(1): 55-61.
47
Asadpour R, Azari M, Hejazi M, et al. Protective effects of garlic aqueous extract (Allium sativum), vitamin E, and N-acetylcysteine on reproductive quality of male rats exposed to lead. Vet Res Forum 2013; 4(4): 251-257.
48
Mirfard M, Johari H, Mokhtari M, et al. The effect of hydro-alcoholic garlic extract on testis weight and spermatogenesis in mature male rats under chemotherapy with cyclophosphamide. J Fasa Uni Med Sci 2011; 1(3):123-130.
49
Oi Y, Imafuku M, Shishido C, et al. Garlic supplementation increases testicular testosterone and decreases plasma corticosterone in rats fed a high protein diet. J Nutr 2001; 131(8): 2150-2156.
50
ORIGINAL_ARTICLE
Molecular detection of Nosema ceranae in the apiaries of Kurdistan province, Iran
Nosema disease is one of the most important diseases of adult honey bees worldwide. It is known as silent killer because there are no characteristic symptoms. The aim of the present study was to determine prevalence of Nosema species in various towns of Kurdistan province in Iran. A multiplex polymerase chain reaction (multiplex-PCR) was performed for identification of Nosema species infecting European honeybee, Apis mellifera. A total of 100 samples were collected from apiaries (870 hives) in 10 counties of Kurdistan province, located in the west of Iran. Samples were examined using light microscope and PCR. The light microscope was used to determine the presence of Nosema spores in all of the collected samples. Multiplex-PCR based on 16S ribosomal RNA was used to differentiate N. apis from N. ceranae. Overall prevalence of the microscopic evaluation and PCR method were 29.00% and 32.00%, respectively. The analysis of Nosema isolates from interrogation of DNA databank entries of Kurdistan apiaries (based on rRNA sequence data) indicated that only N. ceranae was widespread in these apiaries, and it had already been found in high percentages (50.00%) in Marivan and Kamiaran counties of Kurdistan province. It was shown that only N. ceranae was found by PCR assay in the region.
https://vrf.iranjournals.ir/article_32086_1f12e2862ba6e18d849350dcf66fee58.pdf
2018-09-15
273
278
10.30466/vrf.2018.32086
Iran
Kurdistan
Molecular identification
Nosema ceranae
Nosemosis
Mohammad
Khezri
m.khezri@areo.ir
1
Department of Veterinary Research, Kurdistan Agricultural and Natural Resources Research Center, AREEO, Sanandaj, Iran
LEAD_AUTHOR
Mojtaba
Moharrami
moharrami@yahoo.com
2
Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
AUTHOR
Hossain
Modirrousta
3
Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
AUTHOR
Maryam
Torkaman
m.torkaman2013@gmail.com
4
Department of Honey Bee, Silk Worm and Wildlife Research Diseases, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
AUTHOR
Saleh
Salehi
salehi1361@yahoo.com
5
Department of Veterinary Research, Kurdistan Agricultural and Natural Resources Research Center, AREEO, Sanandaj, Iran
AUTHOR
Babak
Rokhzad
babakrokhzad1396@yahoo.com
6
Department of Veterinary Research, Kurdistan Agricultural and Natural Resources Research Center, AREEO, Sanandaj, Iran
AUTHOR
Homan
Khanbabai
7
Department of Veterinary Research, Kurdistan Agricultural and Natural Resources Research Center, AREEO, Sanandaj, Iran
AUTHOR
Fries I. Nosema ceranae in European honey bees (Apis mellifera). J Invertebr Pathol 2010;103 (S1): S73-79.
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Fries I, Feng F, da Silva A, et al. Nosema ceranae n. sp. (Microspora, Nosematidae), morphological and molecular characterization of a microsporidian parasite of the Asian honey bee Apis ceranae (Hymenoptera, Apidae). Eur J Protistol 1996;32(3):356-365.
4
Huang W-F, Jiang J-H, Chen Y-W, et al. A Nosema ceranae isolate from the honeybee Apis mellifera. Apidologie 2007;38(1):30-37.
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Paxton RJ, Klee J, Korpela S, et al. Nosema ceranae has infected Apis mellifera in Europe since at least 1998 and may be more virulent than Nosema apis. Apidologie 2007;38(6):558-565.
6
Chen Y, Evans JD, Smith IB, et al. Nosema ceranae is a long-present and wide-spread microsporidian infection of the European honey bee (Apis mellifera) in the United States. J Invertebr Pathol 2008;97(2):186-188.
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Chen YP, Huang ZY. Nosema ceranae, a newly identified pathogen of Apis mellifera in the USA and Asia. Apidologie 2010;41(3):364-374.
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Fries I, Martín R, Meana A, et al. Natural infections of Nosema ceranae in European honey bees. J Apic Res 2006;47(3):230-233.
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Tapaszti Z, Forgách P, Kövágó C, et al. First detection and dominance of Nosema ceranae in Hungarian honeybee colonies. Acta Vet Hung 2009;57:383-388.
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Bourgeois LA, Rinderer TE, Beaman LD, et al. Genetic detection and quantification of Nosema apis and N. ceranae in the honey bee. J Invertebr Pathol 2010;103(1):53-58.
14
Cox-Foster DL, Conlan S, Holmes EC, et al. A metagenomic survey of microbes in honey bee colony collapse disorder. Science 2007;318(5848):283-287.
15
Martín-Hernández R, Meana A, Prieto L, et al. Outcome of colonization of Apis mellifera by Nosema ceranae. Appl Environ Microbiol 2007;73(20):6331-6338.
16
Bokaie S, Sharifi L, Mehrabadi M. Prevalence and epizootical aspects of varroasis in Golestan province, northern Iran. J Arthropod Borne Dis 2014;8(1):102-107.
17
Fries I, Chauzat MP, Chen YP, et al. Standard methods for Nosema research. J Apic Res 2013;52(1):1-28.
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Hall T. BioEdit: An important software for molecular biology. GERF Bull Biosci 2011;2(1):60-61.
19
Lotfi AR, Jamshidi R, Aghdam Shahryar H, et al. The Prevalence of nosemosis in honey bee colonies in Arasbaran Region (Northwestern Iran). Am Eurasian J Agric Environ Sci 2009;5(2):255-257.
20
Modirrousta H, Moharrami M, Mansouri MA. Retrospective study of the Nosema ceranae infection of honey bee colonies in Iran (2004-2013). Arc Razi Ins 2014;69(2):197-200.
21
Nabian S, Ahmadi K, Nazem Shirazi M, et al. First detection of Nosema ceranae, a microsporidian protozoa of European honeybees (Apis mellifera) in Iran. Iran J Parasitol 2011;6(3):89-95.
22
Tavassoli M, Eiganinejad S, Alizadeh-Asl S. A survey on Nosema apis infection in apiaries of Urmia, North-West of Iran. Iran J Vet Sci Technol 2010;1(1):35-40.
23
Razmaraii N, Karimi H. A survey of Nosema disease of honey bee (Apis mellifera) in East Azarbaijan province of Iran. J Anim Vet Adv 2010;9(5):879-882.
24
Aroee F, Azizi H, Shiran B, et al. Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran). Asian Pac J Trop Biomed 2017;7(1):10-13.
25
Davoudi J, Naderi A, Mohammadpour F, et al. Study of infection rate of suburb bee hives to parasites nosema apis, varroa spp. and acarapis woodi in Miyaneh, Iran. J Now Agric Sci 2009;4(13):39-43.
26
Botias C, Martin-Hernandez R, Garrido-Bailon E, et al. The growing prevalence of Nosema ceranae in honey bees in Spain, an emerging problem for the last decade. Res Vet Sci 2012;93(1):150-155.
27
Williams GR, Shafer AB, Rogers RE, et al. First detection of Nosema ceranae, a microsporidian parasite of European honey bees (Apis mellifera), in Canada and central USA. J Invertebr Pathol 2008;97(2):189-192.
28
COLOSS. Nosema disease: lack of knowledge and work standardization. COST Action FA0803 - Prevention of honeybee colony losses. Guadalajava, Spain 2009:1-39.
29
Higes M, Martín Hernández R, García Palencia P, et al. Horizontal transmission of Nosema ceranae (Micro-sporidia) from worker honeybees to queens (Apis mellifera). Environ Microbiol Rep 2009;1(6):495-498.
30
Higes M, Meana A, Bartolomé C, et al. Nosema ceranae (Microsporidia), a controversial 21st century honey bee pathogen. Environ Microbiol Rep 2013;5(1):17-29.
31
Higes M, García-Palencia P, Martín-Hernández R, et al. Experimental infection of Apis mellifera honeybees with Nosema ceranae (Microsporidia). J Invertebr Pathol 2007;94(3):211-217.
32
Martín Hernández R, Botías C, Bailón EG, et al. Microsporidia infecting Apis mellifera: coexistence or competition. Is Nosema ceranae replacing Nosema apis? Environ Microbiol 2012;14(8):2127-2138.
33
Martínez J, Leal G, Conget P. Nosema ceranae an emergent pathogen of Apis mellifera in Chile. Parasitol Res 2012;111(2):601-607.
34
Budge G, Powell M, Roberts K, et al. What has Nosema got to do with losses? Monitoring both Nosema species in the UK. In: Kence M (Ed). 4th European Conference of Apidology. Ankara, Turkey 2010:47.
35
Gisder S, Hedtke K, Mo¨ckel N, et al. Five-year cohort study of Nosema spp. in Germany: Does climate shape virulence and assertiveness of Nosema ceranae? Appl Environ Microbiol 2010;76(9):3032-3038.
36
Bermejo FJO, Fernández PG. Nosema disease in the honey bee (Apis mellifera L) infested with varroa mites in southern Spain. Apidologie 1997;28:105-112.
37
Pajuelo AG, Torres C, Bermejo FJO. Colony losses: A double blind trial on the influence of supplementary protein nutrition and preventative treatment with fumagillin against Nosema ceranae. J Apic Res 2008;47:84-86.
38
Runckel C, Flenniken ML, Engel JC, et al. Temporal analysis of the honey bee microbiome reveals four novel viruses and seasonal prevalence of known viruses, Nosema, and Crithidia. PLoS One 2011;6(6):e20656.
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Bailey L. The epidemiology and control of nosema disease of the honeybee. Ann Appl Biol 1955; 43:379-389.
40
Martín-Hernández R, Meana A, García-Palencia P, et al. Effect of temperature on the biotic potential of honeybee microsporidia. Appl Environ Microbiol 2009;75(8):2554-2557.
41
Martín-Hernández R, Meana A, García-Palencia P, et al. Effect of temperature on the biotic potential of honeybee microsporidia. Appl Environ Microbiol 2009;75(8):2554-2557.
42
ORIGINAL_ARTICLE
Molecular detection and phylogenetic properties of isolated infectious bronchitis viruses from broilers in Ahvaz, southwest Iran, based on partial sequences of spike gene
Infectious bronchitis (IB) is a highly contagious disease involving mostly upper respiratory tract in chickens, leading to significant economic losses in the poultry industry worldwide. One of the major concerns regarding to IB is the emergence of new types of infectious bronchitis viruses (IBVs). The purpose of this study was to identify the IBVs isolated from Iranian broiler chickens with respiratory symptoms. Twenty-five broiler flocks around Ahwaz (southwest of Iran) were examined for IBV. The specimens including trachea, lung, liver, kidney, and ceacal tonsil, were collected from diseased birds and inoculated into chicken embryonated eggs. Harvested allantoic fluids were subjected to reverse transcription polymerase chain reaction (RT-PCR) using primers in order to amplify spike 1 (S1) gene of IBV. The RT-PCR products of four IBV isolates were sequenced. The results showed that from 25 examined flocks with respiratory disease, 12 flocks (48.00%) were positive for IBV. In phylogenetic analysis, our isolates were closely related to the QX-like viruses such as PCRLab/06/2012 (Iran), QX, HC9, HC10, CK/CH/GX/NN11-1, CK/CH/JS/YC11-1, CK/CH/JS/2010/13, CK/CH/JS/2011/2 (China), QX/SGK-21, QX/SGK-11 (Iraq) with nucleotide homology up to 99.00%. This study indicates the role of IBVs in the respiratory disorders of broiler flocks located in southwest Iran, and also the existence of a variant of IBV, which is distinguishable from the other Iranian variants.
https://vrf.iranjournals.ir/article_32089_c9e1272856055b6407b2413d2c9965e7.pdf
2018-09-15
279
283
10.30466/vrf.2018.32089
Infectious bronchitis virus
Iran
QX-like viruses
S1 gene
zahra
boroomand
z.boroomand@scu.ac.ir
1
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
LEAD_AUTHOR
Ramezan Ali
Jafari
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Mansour
Mayahi
m_mayahi@yahoo.com
3
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Jackwood MW, de Wit S. Infectious bronchitis. In: Swayne DE, Glisson, JR, McDougald LR, et al. (Eds). Diseases of poultry. 13th ed. Ames, USA: Blackwell Publishing, 2013; 139-159.
1
Cavanagh D. Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. Avian Pathol 2003; 32(6): 567-582.
2
Aghakhan SM, Abshar N, Fereidouni SR, et al. Studies on avian viral infections in Iran. Arch Razi Ins 1994; 44: 1-5.
3
Haqshenas G, Asasi K, Akrami H. Isolation and molecular characterization of infectious bronchitis virus, isolate Shiraz 3. IBV, by RT-PCR and restriction enzyme analysis. Iranian J Vet Res 2005; 6: 9- 15.
4
Boroomand Z, Razeghian I, Asasi K, et al. Isolation and identification of a new isolate of avian infectious bronchitis virus IRFIBV32 and study of its pathogenicity. Online J Vet Res 2011; 15 (4): 366-380.
5
Ahmed Z, Naeem K, Hameed A. Detection and seroprevalence of infectious bronchitis virus strains in commercial poultry in Pakistan. Poultry Sci 2007; 86: 1329-1335.
6
Swayne DE, Glisson JR, Jackwood MW, et al. A Laboratory manual for the isolation and identification of avian pathogens. 4th ed. Texas, USA: American Association of Avian Pathologists. 1998; 169-174.
7
Liu S, Kong X. A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China. Avian Pathol 2004; 33: 321-327.
8
Adzhar A, Gough RE, Haydon D, et al. Molecular analysis of the 793/B serotype of infectious bronchitis virus in Great Britain. Avian Pathol 1997; 26(3): 625-640.
9
DeWit JJ, Cook JK. Factors influencing the outcome of infectious bronchitis vaccination and challenge experiments. Avian Pathol 2014; 43: 485-497.
10
Yu L, Wang Z, Jiang Y, et al. Molecular epidemiology of infectious bronchitis virus isolates from China and Southeast Asia. Avian Dis 2001; 45: 201-209.
11
Seyfi Abad Shapouri MR, Mayahi M, Assasi K, et al. A survey of the prevalence of infectious bronchitis virus type 4/91 in Iran. Acta Vet Hung 2004; 52(2): 163-166.
12
Hosseini, H, Fard MH, Charkhkar S, et al. Epidemiology of avian infectious bronchitis virus genotypes in Iran (2010-2014). Avian Dis 2015; 59(3):431-435.
13
Modiri Hamadan A, Ghalyanchi Langroudi A, Hashemzadeh M, et al. Genotyping of Avian infectious bronchitis viruses in Iran (2015-2017) reveals domination of IS-1494 like virus. Virus Res 2017; 15(240): 101-106.
14
Najafi H, Ghalyanchi Langroudi A, Hashemzadeh M, et al. Molecular characterization of infectious bronchitis viruses isolated from broiler chicken farms in Iran, 2014-2015. Arch Virol 2016; 161(1):53-62.
15
Sasipreeyajan TPJ. The pathogenesis of a new variant genotype and QX-like infectious bronchitis virus isolated from chickens in Thailand. Thai J Vet Med 2012; 42: 51-57.
16
Amin OG, Valastro V, Salviato A, et al. Circulation of QX-like infectious bronchitis virus in the Middle East. Vet Rec 2012; 171:530. doi: 10.1136/vr.100896
17
Seger W, Ghalyanchi Langeroudi A, Karimi V, et al. Genotyping of infectious bronchitis viruses from broiler farms in Iraq during 2014-2015. Arch Virol 2016; 161(5): 1229-1237.
18
Bozorgmehri-Fard M, Charkhkar S, Hosseini H. Detection of the Chinese genotype of infectious bronchitis virus (QX-type) in Iran. Iran J Virol 2014; 7: 21-24.
19
Mahmood ZH, Sleman RR, Uthman AU. Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol 2011; 150: 21-27.
20
ORIGINAL_ARTICLE
Multicentric lymphoma in a Rottweiler dog with bilateral ocular involvement: A case report
A 10-year-old, male Rottweilerdog was presented to Small Animal Hospital of Tehran University with a history of lethargy, anorexia, weight loss, vomiting, polyuria, polydipsia and blindness. The dog showed symptoms of depression, high body temperature (39.2 ˚C), tachypnea (40 breaths min-1) and cachexia. In ophthalmic examination, bilateral hyphema (hemmorrahge in anterior chamber of the eye) and blindness were detected. The pulse of the animal was normal (90 beats min-1) and obvious general lymphadenopathy was determined. Lymph nodes were firm, freely movable, and painless on palpation. Initial differential diagnosis of lymphoma included lymphoma, metastatic neoplasia, lymphoid hyperplasia, and lymphadenitis. In hematology and cytology tests, this case was suspected to lymphoma. Immunohistochemical staining of neoplastic lymph node revealed that nearly 20.00 – 25.00% of neoplastic cells were strongly positive for anti CD3, whereas they were negative for both CD20 and CD79a. According to the microscopic and immunohistochemical findings, a diagnosis of T cell lymphoma was made. The present report is the first case of multicentric lymphoma with ocular metastasis in a dog in Iran.
https://vrf.iranjournals.ir/article_32090_540fad5ae2c0f2b6b57198f9685ae074.pdf
2018-09-15
285
288
10.30466/vrf.2018.32090
Hyphema
Lymphoma
Multicentric
Rottweiler dog
Hamed
Mansour Lakooraj
1
Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
AUTHOR
Mahmood
Ahmadi-hamedani
ahmadi.hamedani@semnan.ac.ir
2
Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran
LEAD_AUTHOR
Omid
Dezfoulian
dezfoulian.o@lu.ac.ir
3
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
Masoud
Selk Ghaffari
selkghaffari@gmail.com
4
Department of Clinical Sciences, College of Veterinary Medicine, Karaj Branch, Islamic Azad University, Alborz, Iran
AUTHOR
Rallis T, Koutinas A, Lekkas S, et al. Lymphoma (malignant lymphoma, lymphosarcoma) in the dog. J Small Anim Pract. 1992; 33(12): 590-596.
1
Vail DM, MacEwen EG, Young KM. Canine lymphoma and lymphoid leukemias. In: Withrow SJ, MacEwen EG (Eds). Small animal clinical oncology. 3rd ed. Philadelphia, USA: WB Saunders 2001; 558-580.
2
Sequeira JL, Franco M, Bandarra EP, et al. Anatomoclinical characteristics of canine lymphomas in the region of Botucatu, São Paulo [Portuguese]. Arq Bras Med Vet Zootec 1999; 51(3): 245- 250.
3
Nelson RW, Couto CG. Small animal internal medicine. 2nd ed. St. Louis, USA: Mosby 1998; 1123-1133.
4
Starrak GS, Berry CR, Page RL, et al. Correlation between thoracic changes and remission/survival duration in 270 dogs with lymphosarcoma. Vet Radiol Utrasound. 1997; 38(6): 411-418.
5
Teske E, Heerde PV, Rutteman GR, et al. Prognostic factors for treatment of malignant lymphoma in dogs. J Am Vet Med Assoc. 1994; 205(12): 1722-1728.
6
Milner RJ, Pearson J, Nesbit JW, et al. Immuno-phenotypic classification of canine malignant lymphoma on formalin-fixed paraffin wax-embedded tissue by means of CD3 and CD79a cell markers. J Vet Res 1996; 63(4): 309-313.
7
Fournel-Fleury C, Ponce F, Felman P, et al. Canine T-cell lymphomas: A morphological, immunological, and clinical study of 46 new cases. Vet Pathol 2002; 39(1): 92-109.
8
Hosoya K, Kisseberth WC, Lord LK, et al. Comparison of COAP and UW‐19 protocols for dogs with multicentric lymphoma. J Vet Intern Med. 2007; 21(6): 1355-1363.
9
Nelson RW, Couto CG. Small animal internal medicine 5th ed. St. Louis, USA: Mosby 2013; 1160-1174.
10
ORIGINAL_ARTICLE
Metastatic giant cell osteosarcoma in a cat
A four-year-old male Persian cat was referred with three weeks history of progressive lameness due to a rigid osseous mass with 3.50×2.50×2.00 cm in dimensions in his left arm. In the histopathological evaluation of bone biopsy, two distinct populations of cells including multinucleated giant cells and oval cells which embedded in a fibro-osseous stroma and surrounded by lamellar bone trabeculae were observed. At necropsy, multiple metastatic nodules with different sizes unveiled in the liver and spleen. Microscopically, those tumor cells which already described in the bone lesion were also infiltrated to the liver and spleen. The neoplastic cells had no immunoreaction to CD68, desmin, alpha smooth muscle actin, S100, CD20, CD3 and pancytokeratin, but only a few cells had reaction to vimentin were similar to fibroblasts.
https://vrf.iranjournals.ir/article_32087_ed2f446ff05c191c82f283f597c6c365.pdf
2018-09-15
289
292
10.30466/vrf.2018.32087
Giant cell osteosarcoma
Immunohistochemistry
Metastasis
Persian cat
Ghasem
Farjanikish
farjanikish.gh@lu.ac.ir
1
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
LEAD_AUTHOR
Omid
Dezfoulian
dezfoulian.o@lu.ac.ir
2
Department of Pathobiology, Faculty of Veterinary Medicine, Lorestan University, Khorramabad, Iran
AUTHOR
Hossein
Mohammadi
mohammadi.h@gmail.com
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Department of Pathology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
AUTHOR
Fletcher CDM, Unni KK, Mertens F. World health organization classification of tumours. Pathology and genetics of tumours of soft tissue and bone. Lyon, France: International Agency for Research on Cancer (IARC) 2002; 264-286.
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Verma RK, Gupta G, Bal A, et al. Primary giant cell rich osteosarcoma of maxilla: An unusual case report. J Oral Maxillofac Surg 2011; 10: 159-162.
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Holmberg BJ, Farese JP, Taylor D, et al., Osteosarcoma of the humeral head associated with osteocondritis dissecans in a dog. J Am Anim Hosp Assoc 2004; 40(3): 246-249.
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Oryan A, Sadoughifar R, Shirian S, et al. Giant cell-rich osteosarcoma of tibia in a dog: a pathological and immunohistochemical study. Comp Clin Pathol 2015; 24: 177-179.
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Cristo TG, Vargas CB, Biezus G, et al. Metastatic osteosarcoma as a cause of hemorrhagic stroke in a dog. Braz J Vet Pathol 2017; 10(3): 105-110
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Kirpensteijn J, Van den Bos R, Endenburg N. Adaption of dogs to the amputation of a limb and their owners’ satisfaction with the procedure. Vet Rec 1999; 144: 115-118.
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Brodey, RS; Riser, WH. Canine osteosarcoma: A clinicopathologic study of 194 cases. Clinical Orthopedics 1969; 62(4): 26-32.
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Mueller F, Fuchs B, Kaser-Hotz B. Comparative biology of human and canine osteosarcoma. Anticancer Res 2007; 27(1A):155-164.
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Cerilli L, Wick M. Immunohistology of soft tissue and osseous neoplasms. In: Dabbs DJ (Ed). Diagnostic immunohistochemistry. New York, USA: Chruchill Livingstone 2002; 72-73.
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