TY - JOUR ID - 1529 TI - Effects of Bunium persicum (Boiss.) Essential Oil on the Contractile Responses of Smooth Muscle (An in vitro Study) JO - Veterinary Research Forum JA - VRF LA - en SN - 2008-8140 AU - Jalilzadeh-Amin, Ghader AU - Maham, Massoud AU - Dalir-Naghadeh, Bahram AU - Kheiri, Farshad AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AD - Department of Medicinal and Industrial Plants, Institute of Biotechnology, Urmia University, Urmia, Iran Y1 - 2011 PY - 2011 VL - 2 IS - 2 SP - 87 EP - 96 KW - Bunium persicum KW - essential oil KW - Smooth muscle contraction KW - Rumen KW - Abomasum DO - N2 - Bunium persicum (Boiss.) is an economically important medicinal plant growing wild in arid regions in Iran. The essential oil of B. persicum (EOBP) was extracted using hydrodistillation. A total of eighteen compounds, representing 96.14 % of the oil was identified by gas chromatography/mass spectrometry (GC/MS).The main compounds were cuminaldehyde (23.04 %), gamma-terpinene (14.48 %), trans-3-Caren-2-ol (12.51 %), acetic acid (10.90 %) and 1,3,8 -p- menthatriene (7.89 %). The effects of 0.1 to 1000 g mL-1 EOBP on ruminal and abomasal smooth muscle of twenty-four healthy sheep and ileum preparations of six rats were assessed in vitro. Ruminal preparations showed relaxation (P < 0.05) when exposed to 100 - 1000 g mL-1 concentrations of EOBP. In the isolated abomasal preparations, EOBP (0.1 - 1 g mL-1) represented a weak spasmogenic effect followed by relaxation. The spontaneous contraction of abomasal smooth muscles was completely abolished with a high dose (1000 g mL-1) of EOBP. Five-minute incubation with EOBP, significantly (P < 0.05) inhibited Ach-induced contraction in higher doses on both tissues. In contrast, rat ileum only showed dose-dependent relaxation effect, and pre-incubated tissues with EOBP, decreased the Ach-induced contraction. The data indicated that the plant contained spasmogenic and spasmolitic constituents. The results also showed that the EOBP profoundly alters gastrointestinal smooth muscle contraction in a dose-dependent and tissue-specific manner. UR - https://vrf.iranjournals.ir/article_1529.html L1 - https://vrf.iranjournals.ir/article_1529_10e361b33c357ba41b898bede489653e.pdf ER -