TY - JOUR ID - 21610 TI - Prevalence and antibiotic resistance profile of thermophilic Campylobacter spp. of slaughtered cattle and sheep in Shiraz, Iran JO - Veterinary Research Forum JA - VRF LA - en SN - 2008-8140 AU - Khoshbakht, Rahem AU - Tabatabaei, Mohammad AU - Hoseinzadeh, Saeid AU - Raeisi, Mojtaba AU - Shirzad Aski, Hesamaddin AU - Berizi, Enayat AD - Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran AD - Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran AD - Department of Food Hygiene, School of Veterinary Medicine, Shiraz University, Shiraz, Iran AD - Department of Public Health, School of Health, Golestan University of Medical Sciences, Gorgan, Iran Y1 - 2016 PY - 2016 VL - 7 IS - 3 SP - 241 EP - 246 KW - Antibiotic resistance KW - Cattle KW - PCR KW - Sheep KW - Thermophilic Campylobacter spp DO - N2 - Althoughpoultry meat is considered as the main source for human Campylobacter infections,there is limited information about non-poultry sources. The present study was aimed to investigate the prevalence and the antibiotic resistance of thermophilic Campylobacter spp. in fecal samples of the cattle and sheep in Shiraz, Iran. A total of 302fecal samples were obtained from clinically healthy, slaughtered cattle and sheep from Shiraz slaughterhouse. The animals were clinically healthy before being slaughtered. The samples were cultured according to the specific cultivation method under thermophilic conditions. The susceptibility of Campylobacter isolates were determined for 13 antimicrobial agents. All enriched samples and cultured isolates were targeted for polymerase chain reaction (PCR) detection of 16S rRNA and multiplex PCR for determining their species. Among 302 fecal samples, 65 (21.5%) and 205 (67.8%) samples were positive for the presence of Campylobacter species with the cultivation and PCR techniques, respectively. All 65 distinct isolates were susceptible to neomycin and colistin and the isolates showed high resistance to cephalotin (83.0%) and ciprofloxacin (67.7%). After the multiplex PCR, 78.5% of total positive samples showed the simultaneous presence of Campylobacter jejuni and Campylobacter coli. In conclusion, the results emphasized that non-poultry farms are important as a possible source of Campylobacter infections. UR - https://vrf.iranjournals.ir/article_21610.html L1 - https://vrf.iranjournals.ir/article_21610_74b889db6e1db8bb92b8d58dc44df984.pdf ER -