TY - JOUR ID - 35902 TI - Differences between first and second ejaculations regarding seminal plasma calcium, magnesium and total antioxidant capacity in dual-purpose Fleckvieh bulls and their relationships with semen quality JO - Veterinary Research Forum JA - VRF LA - en SN - 2008-8140 AU - Khaki, Amir AU - Araghia, Atefeh AU - Lotfi, Mehdi AU - Nourian, Alireza AD - Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran AD - Iran Simmental Cattle Breeding Center, Amard-dam Company, Amol, Iran Y1 - 2019 PY - 2019 VL - 10 IS - 4 SP - 333 EP - 341 KW - Calcium KW - Magnesium KW - Repetitive ejaculations KW - Semen quality KW - Seminal plasma DO - 10.30466/vrf.2018.89223.2159 N2 - Artificial insemination is a well-established and widely used method for genetic improvement in cattle breeding industry. Recently, researchers have shown an increased interest in the cryoprotective effects of minerals and antioxidants on semen. Previous studies on calcium (Ca) and magnesium (Mg), two main macro-minerals, have mainly investigated their roles in mammalian spermatogenesis and fertility. In addition, the experimental data examining the semen content regarding these minerals and antioxidants from different animal species are rather controversial and there is no general agreement about their associations with semen quality. Therefore, this study was conducted to assess the seminal plasma concentrations of Ca, Mg and total antioxidant capacity (TAC) in first and second ejaculations of dual-purpose Fleckvieh bulls and to link them to the sperm characteristics of fresh and frozen-thawed semen. Sperm progressive motility after thawing was used to classify the data into three groups: < 40.00%, 40.00 to 50.00% and > 50.00%. The measurements of two minerals and TAC were carried out using spectrophotometry and enzyme-linked immunosorbent assays, respectively. The results showed that there were significant differences in several parameters of semen quality between first and second ejaculations. No significant differences were also found on Ca and Mg concentrations and Ca/Mg ratio. The TAC level was significantly higher in the first ejaculation than the second one. The findings of this study suggest that TAC is a potential marker for bull semen quality assessment in the frozen semen production industry. UR - https://vrf.iranjournals.ir/article_35902.html L1 - https://vrf.iranjournals.ir/article_35902_12f2eb9a14e5ae7f8116dae2f513f426.pdf ER -