TY - JOUR ID - 5395 TI - Effects of diluting medium and holding time on sperm motility analysis by CASA in ram JO - Veterinary Research Forum JA - VRF LA - en SN - 2008-8140 AU - Mostafapor, Somayeh AU - Farrokhi Ardebili, Farhad AD - Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran Y1 - 2014 PY - 2014 VL - 5 IS - 2 SP - 101 EP - 105 KW - CASA KW - Ram KW - Semen diluters KW - Sperm motility DO - N2 - The aim of this study was to evaluate the effects of dilution rate and holding time on various motility parameters using computer-assisted sperm analysis (CASA). The semen samples were collected from three Ghezel rams. Samples were diluted in seminal plasma (SP), phosphate-buffered saline (PBS) containing 1% bovine serum albumin (BSA) and Bioexcell. The motility parameters that computed and recorded by CASA include curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), straightness (STR), linearity (LIN), amplitude of lateral head displacement (ALH), and beat cross frequency (BCF). In all diluters, there was a decrease in the average of all three parameters of sperms movement velocity as the time passed, but density of this decrease was more intensive in SP. The average of ALH between diluters indicated a significant difference, as it was more in Bioexcell in comparison with the similar amount in SP and PBS. The average of LIN in the diluted sperms in Bioexcell was less than two other diluters in all three times. The motility parameters of the diluted sperms in Bioexcell and PBS indicated an important and considerable difference with the diluted sperms in SP. According to the gained results, the Bioexcell has greater ability in preserving motility of sperm in comparison with the other diluters but as SP is considered as physiological environment for sperm. It seems that the evaluation of the motility parameters in Bioexcell and PBS cannot be an accurate and comparable evaluation with SP. UR - https://vrf.iranjournals.ir/article_5395.html L1 - https://vrf.iranjournals.ir/article_5395_9f46ff4a033676ae72bcd3d5f0436849.pdf ER -