TY - JOUR ID - 6621 TI - Evaluation of antioxidant effects of crocin on sperm quality in cyclophosphamide treated adult mice JO - Veterinary Research Forum JA - VRF LA - en SN - 2008-8140 AU - Bakhtiary, Zahra AU - Shahrooz, Rasoul AU - Ahmadi, Abbas AU - Zarei, Leila AD - Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran AD - Maternal and Childhood Obesity Research Center, Urmia University of Medical Sciences, Urmia, Iran Y1 - 2014 PY - 2014 VL - 5 IS - 3 SP - 213 EP - 218 KW - Crocin KW - Cyclophosphamide KW - Mice KW - Sperm quality DO - N2 - Cyclophosphamide (CP) is one of the anti-neoplastic drugs. Despite its numerous clinical applications, it has devastating effects on the testicles and declines the sperm quality in treated patients. This study was aimed to investigate the protective effect of crocin in improving the toxicity induced by CP in reproductive system. In this study, 24 male adult mice (6 to 8 weeks) were randomly divided into three groups, control group received normal saline (0.1 mL, IP, daily), the CP group received CP (15 mg kg-1, IP, weekly) and the CP + crocin group received CP along with crocin (200 mg kg-1, IP, daily). After 35 days of treatment, animals were sacrificed. The samples of epididymis in human tubal fluid medium incubated for 30 min in 5% CO2 for flotation of sperm. Sperm were obtained from caudal epididymis using dissecting method. Then, the parameters of sperm quality including sperm count, motility, viability, DNA damage, nuclear maturation, and sperm morphology were evaluated. In CP group, the sperm count, motility, viability, nuclear maturation and sperm morphology were significantly decreased compared to control group (p < 0.05) and in the CP + crocin group all of these parameters significantly increased compared to CP group (p < 0.05). The percentage of sperm with DNA damage in the CP group significantly increased compared to other groups (p < 0.05). The results of this study indicated that the crocin was able to suppress free radicals and enhance the quality of sperm in CP treated animals. UR - https://vrf.iranjournals.ir/article_6621.html L1 - https://vrf.iranjournals.ir/article_6621_9faea536b9bca65b75e31aebb2bd9258.pdf ER -