2024-03-29T06:28:41Z
https://vrf.iranjournals.ir/?_action=export&rf=summon&issue=2343
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Histomorphometric study of brachiocephalic artery of Japanese quail
Sarah
Shariati
Farhad
Rahmanifar
Amin
Tamadon
Brachiocephalic arteries in quails are large arteries which are arising separately from the aortic arch. The aim of the present study was to determine the histomorphometric aspects of brachiocephalic arteries in the Japanese quail. The different layers of the brachiocephalic artery were studied quantitatively in 10, 20 and 60 days-old Japanese quail; (n = 6) and both sexes. Luminal diameter, thickness of the intima, media and adventitia, the percentage of the intima, media and adventitia, as compared with the total wall thickness were determined. It was found that luminal diameter and whole artery thickness increased by age (p < 0.05). In addition, the tunica media was the thickest layer, then tunica intima and at last tunica adventitia (p < 0.05). The muscularity of the right brachiocephalic artery was more than that of the left one (p < 0.05). Histomorphometric study of brachiocephalic arteries of Japanese quails showed that increasing of age causes increase of internal and external diameters of the artery and this increase in females was more than males.
age
Brachiocephalic artery
Japanese quail
sex
Histomorphometry
2015
09
01
185
190
https://vrf.iranjournals.ir/article_14309_d136a14cbedbae5e890678642b7a156b.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Synbiotic enhances immune responses against infectious bronchitis, infectious bursal disease, Newcastle disease and avian influenza in broiler chickens
Alireza
Talebi
Amir
Amani
Masoud
Pourmahmod
Poya
Saghaei
Reza
Rezaie
Increased susceptibility of birds to avian pathogens in intensive husbandry system has emphasized on necessity of improvement of innate and specific immune responses of birds by the fast establishment of a beneficial microflora and immune stimulator factors to guarantee healthy and low-price products. During this study, 192 one-day-old broiler chicks (Ross-380) in four groups with three replicates per group were used to investigate effectiveness of synbiotic Biomin Imbo on immune responses of the chickens following routine vaccination against Newcastle disease (ND), avian influenza (AI), infectious bronchitis (IB) and infectious bursal disease (IBD). The results of this study indicated that supplementation of Biomin Imbo in diet enhanced humoral immune responses significantly in the case of ND, IB, IBD (p = 0.049, p = 0.020, p = 0.036, respectively), but insignificantly in the case of AI (p = 0.160) following vaccination of the chickens against these most common important viral poultry diseases. It was more effective following vaccination with live than killed vaccines. In conclusion, application of synbiotic Biomin Imbo, as a feed-additive adjuvant promotes acquired humoral immune responses of broiler chickens.
Avian influenza
Biomin Imbo
Infectious bronchitis
Infectious bursal disease
Newcastle disease
2015
09
01
191
197
https://vrf.iranjournals.ir/article_14313_e0df2f8758a015189e75d91f122d9ddd.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Detection of Arctic and European cluster of canine distemper virus in north and center of Iran
Somayeh
Namroodi
Amir
Rostami
Keyvan
Majidzadeh-Ardebili
Arash
Ghalyanchi Langroudi
Abbas
Morovvati
Canine distemper virus (CDV) creates a very contagious viral multi-systemic canine distemper (CD) disease that affects most species of Carnivora order. The virus is genetically heterogeneous, particularly in section of the hemagglutinin (H) gene. Sequence analysis of the H gene can be useful to investigate distinction of various lineages related to geographical distribution and CDV molecular epidemiology. Since vaccination program is conducted only in large cities of Iran, CD still remains as one of the major causes of death in dogs in this country. In order to monitor H gene, CDV has been detected in 14 out of 19 sampled dogs through the amplification of nucleoprotein (NP) gene in nested-PCR assay. In the next step 665 bp of H gene was amplified in 9 out of 14 NP-gene positive dogs. Phylogenetic analysis distinguished two distinct CDV genotypes in Iran. JN941238 has been embedded in European cluster and JN941239 has been embedded in Arctic cluster. Nucleic analysis has been shown high difference among both Iranian CDV lineages with CDV vaccine strains.
Canine distemper virus
Hemagglutinin gene
Nucleoprotein gene
Phylogenetic analysis
2015
09
01
199
204
https://vrf.iranjournals.ir/article_14306_f371d3cc5998f2cda6a7193c2346cd60.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Effects of tail fat on recovery times of anesthesia with isoflurane in fat-tailed Iranian Lori-Bakhtiyari lambs
Siavash
Sharifi
Abbas
Raisi Sarteshnizi
Farangis
Sharifi
Elham
Yousefian
In the present study, the effect of tail fat on recovery times in intact sheep and sheep with a ligated median sacral artery following similar anesthetic exposure with isoflurane was investigated. This study was performed using seven healthy fat-tailed Iranian Lori-Bakhtiyari ewe lambs. The lambs were anesthetized twice at two week intervals (the experiment was performed in two stages). After mask induction with isoflurane in 100% oxygen, sheep were intubated and anesthesia was maintained for 4 hr using a rebreathing system. Induction and extubation times and time to sternal recumbency and attempts to stand were recorded during anesthetic induction and recovery (Stage 1). Two weeks later, prior to the second anesthesia, the median sacral artery (MSA) was ligated under epidural anesthesia in sheep. All sheep were anesthetized as mentioned above (Stage 2). No significant differences were observed for the induction time between two stages (p > 0.05) but extubation, sternal recumbency and attempts to stand times were significantly longer in intact sheep (Stage 1) after 4 hr anesthesia with isoflurane (p < 0.05). Recovery time was decreased following MSA ligation in fat-tailed sheep, which suggested that body fat had a major role in the recovery time of isoflurane in sheep. We developed an animal model to investigate fat drug solubility of isoflurane gas. Therefore, using less-soluble in fat anesthetics is better than high-soluble anesthetics for prolonged anesthesia to decrease postoperative complication in obese patient.
Anesthesia
Fat-tail
Isoflurane
Lori-Bakhtiyari lamb
Recovery times
2015
09
01
205
209
https://vrf.iranjournals.ir/article_14308_dc843dfbd033e01e2869e98a87e32b3f.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Copper and zinc concentrations in uterine fluid and blood serum during the estrous cycle and pre-pubertal phase in water buffaloes
Sayed Mortaza
Alavi Shoushtari
Siamak
Asri Rezaie
Amir
Khaki
Abulfazl
Belbasi
Hamid
Tahmasebian
To investigate uterine fluid and serum copper (Cu) and zinc (Zn) variations during the estrous cycle in water buffaloes, 71 genital tracts and blood samples were collected from the abattoir in Urmia, Iran. The phase of the estrous cycle was determined by examining ovarian structures; 18, 15, 16 and 22 were pro-estrous, estrous, met-estrous and diestrous, respectively. The uterine fluid was collected by gentle scraping of the uterine mucosa with a curette. Blood serum and uterine fluid samples of 71 pre-pubertal buffalo calves were also collected and treated in similar manners. The mean (± SEM) total serum (77.10 ± 1.50 µg dL-1) and uterine fluid (296.40 ± 9.40 μg dL-1) Cu in cyclic cows was higher than the values of 54.00 ± 1.10 μg dL-1 and 133.40 ± 5.70 μg dL-1 in pre-pubertal calves, respectively. Blood serum (114.60 ± 3.20 μg dL-1) and the uterine fluid (349.90 ± 8.90 μg dL-1) Zn content in cyclic cows were also higher than those (98.80 ± 1.50 μg dL-1 and 246.6 ± 4.50 μg dL-1 respectively) in pre-pubertal calves. Serum Cu in pro-estrus and estrus were lower than those in other stages and also lower than those in the uterine fluid. The lowest serum Zn content was recorded in pro- and met-estrus, while in the uterine fluid it was observed in estrus. In all stages of estrous cycle the uterine fluid Zn content was significantly higher than those of the serum. These results suggested that during the estrous cycle in the buffalo cows, Cu and Zn were actively secreted in uterine lumen and were not dependent on blood serum. The values also increased after puberty.
Buffalo
Estrous cycle
Uterine fluid
copper
Zinc
2015
09
01
211
215
https://vrf.iranjournals.ir/article_14303_7707890f096893047e218fefe5958c30.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Mercury concentrations in common carp (Cyprinus carpio) tissues, sediment and water from fish farm along the Karoun River in Iran
Payvand
Maktabi
Mehran
Javaheri Baboli
Ali Reza
Jafarnejadi
Abolfazl
Askary Sary
The Karoun River is major source of water for warmwater fish culture industry in southwest of Iran. The aim of the present study was to investigate the distribution of mercury in tissues of marketable common carp and in bottom sediments of fish farms in Khouzestan province. This study was carried out on 45 fish farms that are located on the bank of the Karoun River in Khouzestan province, south-west Iran. Concentration of mercury (Hg) was determined using spectrophotometery in three tissues (muscles, liver and gills) of farmed common carp (Cyprinus carpio), water and bottom sediments of fish farms collected from three regions (North, center and south) of the Karoun River, in Khouzestan province, Iran. The concentrations of Hg in muscle tissue (2.71 mg kg-1 dry matter) of fish from the south were significantly higher (p < 0.05) than from the other two sites. In the center and south sampling zones, Hg concentration in muscle was found to be above the maximum tolerable values provided by Food and Drug Administration standards. The Hg concentration of fish farm sediment and water samples were ranged as 0.46 to 0.48 mg kg-1 dry matter and 3.10 to 4.11 μg Hg L-1, respectively. Finally, Hg concentrations at downstream site were higher than upstream site.
Cyprinus carpio
Mercury
Sediment
water
2015
09
01
217
221
https://vrf.iranjournals.ir/article_14305_e64548e68138f5765922748280fe3345.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Prevalence of Listeria monocytogenes in raw milk in Kerman, Iran
Ladan
Mansouri-Najand
Mehrnoush
Kianpour
Masoud
Sami
Maziar
Jajarmi
Listeria monocytogenes as one of the most important pathogen in public health concerns is transmitted through consumption of contaminated food. The pathogen has been considered as a potential source of contamination of raw milk and dairy products. This research was aimed to investigate prevalence of L. monocytogenes in raw milk in Kerman region. In the summer of 2011, a total number of one hundred raw milk samples were collected from bulk tanks of some dairy farms and tested foriap and actA genes using polymerase chain reaction. Among the 100 samples, five isolates (5.0%) were detected as L. monocytogenes based on phenotypic and genotypic characteristics. Considering the low frequency of L. monocytogenes in this study, raw milk cannot be omitted as a potential source of food contamination for the population of the region. To achieve more accurate isolation, identification and control of L. monocytogenes in raw milk, it is suggested that new standard laboratory methods be implemented as well as biosafety outreach programs, management techniques and education.
Food Safety
Kerman
Listeria monocytogenes
Raw Milk
Iran
2015
09
01
223
226
https://vrf.iranjournals.ir/article_14307_3ba7c56e51d4dd9363f294516dcadc68.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
The effect of the hexanic extracts of fig (Ficus carica) and olive (Olea europaea) fruit and nanoparticles of selenium on the immunogenicity of the inactivated avian influenza virus subtype H9N2
Amir Hossein
Asl Najjari
Zolfaghar
Rajabi
Mehdi
Vasfi Marandi
Gholamreza
Dehghan
Influenza is a contagious viral disease that is seen in avian, human and other mammals, so its control is important. Vaccination against influenza virus subtype H9N2 is one of the ways in controlling program, for this reason several vaccines has been produced. Recently, application of inactivated oil-emulsion vaccines in poultry for controlling low pathogenic avian influenza is increasing. At present, oils that are used as adjuvant in commercial vaccines are mineral oils, which not only lack immunizing effect, but also produce some detriments. The aim of this study is the evaluation the immunogenicity of vegetable oils, which are more metabolizable and safer than mineral oils. In this study the efficacy of hexanic extracts of fig (Ficus carica) and olive (Olea europaea) fruit and also nano-selenium on the immunogenicity of the inactivated avian influenza virus subtype H9N2 was evaluated in broiler chickens. The results indicated that the prepared emulsions could elicit a little degree of immunity, but they could not inhibit the anamnestic response and infection. With regard to the results, it seems that the intact mixture of fig and olive fruit hexanic extracts could not be administered as an immunoadjuvant in the vaccine, and about nano-selenium. In spite of positive effect on the immunogenicity of avian influenza virus subtype H9N2, it still needs more work.
Adjuvant
Avian influenza
Fig
Nano-selenium
Olive
2015
09
01
227
231
https://vrf.iranjournals.ir/article_14310_2894a2326b5b67fdb46758895380cc80.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Effects of essential oil of Satureja khuzestanica on the oxidative stress in experimental hyperthyroid male rat
Raheleh
Assaei
Zohreh
Mostafavi-Pour
Naser
Pajouhi
Gholam Hossein
Ranjbar Omrani
Masood
Sepehrimanesh
Fatemeh
Zal
This work analyzes the effects of Satureja khuzestanica essential oil (SKEO) on the thyroid and antioxidant system, assessed by measuring levels of tri-iodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), malondialdehyde (MDA), reduced glutathione (GSH), and glutathione peroxidase (GPx) activity. Forty adult male Sprague Dawley rats (225 ± 25 g) were divided into five equal groups: one control and four hyperthyroid groups that received placebo, 200 mg kg-1 body weight of vitamin (Vit.) E, 225 mg kg-1 body weight of SKEO, 200 and 225 mg kg-1 body weight of Vit. E and SKEO together, respectively. Hyperthyroidism was induced by administering of L-thyroxin in drinking water. After 30 days of L-thyroxin consumption, serum T3 and T4 levels, TSH, and oxidative stress indices were determined. Significant increase in serum T3, T4 and MDA concentrations with a simultaneous significant decrease in TSH, GSH level and GPx activity were observed in hyperthyroid group (p <0.05). In the treatment groups, SKEO and/or Vit. E can compensate serum MDA elevation and GPx activity reduction. Only, SKEO + Vit. E could compensate the decline of GSH levels in response to hyperthyroidism. Supplementation of SKEO, plus Vit. E as antioxidants is useful in attenuating lipid peroxidation and may potentially benefit hyperthyroid patients.
essential oil
Experimental hyperthyroidism
Glutathione
malondialdehyde
Satureja
2015
09
01
233
238
https://vrf.iranjournals.ir/article_14312_33a8ac4e822f8e49722ea0f8b158298d.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Effects of mannan oligosaccharide supplementation on growth, some immune responses and gut lactic acid bacteria of common carp (Cyprinus Carpio) fingerlings
Parvin
Momeni-Moghaddam
Saeed
Keyvanshokooh
Saeed
Ziaei-Nejad
Amir Parviz
Salati
Hossein
Pasha-Zanoosi
This study was conducted to determine the effects of mannan oligosaccharide (MOS) on growth, some immune responses and gut lactic acid bacteria of common carp. Four experimental diets containing 0%, 0.05%, 0.10% and 0.20% MOS were prepared. Each diet was randomly allocated to triplicate groups of fish with initial average weight of approximately 14 g. After eight weeks, survival rate was high in all treatments with no significant difference (p > 0.05). Growth performance including final weight, weight gain (WG) and specific growth rate (SGR) did not differ among the treatments. Feed conversion ratio (FCR) was better when the fish were fed 0.05 to 0.20% MOS diets. The alternative complement activity, lysozyme activity and serum total Ig were found to be significantly (p < 0.05) greater in fish fed 0.20% MOS diets. Although the total intestinal bacterial counts were not affected by dietary treatment (p > 0.05), the lactic acid bacteria levels were significantly elevated in fish fed MOS diets (p < 0.05). These results indicated that oral administration of MOS at 0.20% elevated the immune response, improved FCR and modulated intestinal microbiota of common carp.
Cyprinus carpio
growth
Innate immunity
Intestinal microbiota
Mannan oligosaccharide
2015
09
01
239
244
https://vrf.iranjournals.ir/article_14311_99e3a7670bf4dfd61e9a4a3c336d0bda.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Serological study of small ruminant lentiviruses in sheep population of Khorasan-e-Razavi province in Iran
Behnaz
Norouzi
Alireza
Taghavi Razavizadeh
Mohammad
Azizzadeh
Ashraf
Mayameei
Vahid Najar
Nezhad Mashhadi
Maedi-Visna (MV) virus and caprine arthritis encephalitis (CAE) virus known as small ruminant lentiviruses (SRLVs) cause chronic diseases in susceptible animals. The main reservoirs of these viral agents are sheep and goat. In sheep, MV virus causes a disease as the same name of the virus. This is the first seroprevalence survey of SRLVs in sheep population of Khorasan-e-Razavi province in Iran. Two hundred and twenty sheep from 30 flocks in 12 regions of the province were selected by random cluster sampling method. Serum samples were analyzed for the presence of antibodies against MV/CAE viruses. The seroprevalence in sheep was 34.5% (95.0% CI: 28.3 to 40.7%). Totally, the seroprevalence was in the range of 6.7 to 72.2 %. In 26 flocks of sheep (89.6%; 95.0%CI: 74.4 to 98.8%), at least one seropositive case was detected. The relationship between seropositivity and age, sex, flock size and breeds of sheep were statistically analyzed. In logistic regression model, only age was correlated with SRLV seroprevalence (p < 0.05). This study showed relatively high seroprevalence against SRLVs in sheep population in this area of the country. Due to difficulty in clinical diagnosis, chronic course of the disease, the absence of effective vaccine and treatment and huge economic loss, more epidemiological studies with regards to prevention and control of the disease are necessary.
ELISA
Iran
Khorasan-e-Razavi
Seroprevalence
Small ruminant lentiviruses
2015
09
01
245
249
https://vrf.iranjournals.ir/article_14304_db6852a1464c77dd4022df4cd280989b.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
In vitro histological investigation of interactions between rat decellularized large intestine scaffold and human adipose derived mesenchymal stem cells
Somayeh
Naderi
Malihe
Akbarzadeh Niaki
Nasser
Mahdavi Shahri
Maryam
Moghaddam Matin
Masoud
Fereidoni
Fatemeh
Naseri
The aim of this study was to investigate the interactions between rat intestine decellularized scaffold and human adipose derived mesenchymal stem cells. Rat large intestine was dissected in fragments and decellularized by physicochemical methods. The scaffolds were loaded by human adipose derived mesenchymal stem cells expressing green fluorescent protein. Microscopic sections were prepared from the scaffolds after two weeks of culture with stem cells and studied by histological methods. The interactions of scaffolds with MSCs were also studied by electron microscopy. Histological and electron microscopy studies revealed human mesenchymal stem cell adhesion, migration, division and maintenance during the 14 days of culture in vitro. According to the results, scaffolds prepared from rat intestine matrix could be a suitable scaffold for studying in vitro cell behaviors such as division, migration and attachment. These various behaviors of cultured cells might be due to inductive effects of the extracellular matrix derived scaffold. However, more investigations are required to discover the exact effects of this scaffold and its interactions with mesenchymal stem cells.
Extracellular matrix
Large Intestine
Mesenchymal stem cells
Rat
Scaffold
2015
09
01
251
255
https://vrf.iranjournals.ir/article_14316_6ea685a30d1dca2623f84c9005039762.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Congenital multi-organ malformations in a Holstein calf
Rahim
Hobbenaghi
Bahram
Dalir-Naghadeh
Ali
Nazarizadeh
A 5-day-old female Holstein calf was necropsied because of lethargy, recumbency and anorexia. At necropsy, multiple gross defects were evident in several organs, including unclosed sutures of skull bones, asymmetrical orbits, doming of the skull bones, hydrocephalus, hydranencephaly, cleft palate, brachygnathia, ventricular septal defect, mitral valve dysplasia and rudimentary lungs. On microscopic examination, pulmonary hypoplasia was characterized by reduced number of alveoli, replacement of peri-bronchiolar smooth muscles with connective tissue and small masses of undeveloped cartilage around the small airways. The present report is the first description of the congenital pulmonary hypoplasia accompanied by numerous malformations in Holstein breed.
Calf
Congenital malformation
Necropsy
Pulmonary hypoplasia
2015
09
01
257
260
https://vrf.iranjournals.ir/article_14314_a03198c9ea45264861abc9e0fe3a87ab.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2015
6
3
Abnormal bisubclavian trunk arising from the aortic arch determined by cadaver dissection of a native dog: A case report
Younes
Kamali
Mina Tadjalli
Tadjalli
Congenital anomalies of the great thoracic vessels have been reported in 20% of dogs and cats. In some cases, the vascular ring anomalies remain unrecognized throughout the lifetime of an animal. This report describes a case with an unusual vascular ring anomaly (VRA) that was detected during dissection on a cadaver of an approximately two-year-old male native mixed breed dog. No history of the animal’s life was available. But, good physical condition and age of the animal based on dentition indicated the anomaly was perhaps asymptomatic. Two main branches of the aorta were identified with the initial branch being a bicarotid trunk followed by a bisubclavian trunk. The left subclavian and aberrant right subclavian arteries formed a very short trunk and arose directly from the aortic arch. No dilatation cranial to the esophageal sulcus was found. To the authors' knowledge, our case is the first report of such anomalies perhaps without any clinical signs in a native dog in Iran.
Bisubclavian trunk
Dog
Vascular ring anomaly
2015
09
01
261
263
https://vrf.iranjournals.ir/article_14315_d8d0ef2b2b0d46bd7ee9a59ecbb57357.pdf