2024-03-29T10:15:11Z
https://vrf.iranjournals.ir/?_action=export&rf=summon&issue=3152
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
The effects of pomegranate extract on normal adult rat kidney: A stereological study
Esrafil
Mansouri
John
Basgen
Sadegh
Saremy
Pomegranate (Punica granatum L.) has been used widely in the traditional medicine of various civilizations for more than 5000 years. The pomegranate tree has several parts; each part has useful medicinal effects. Previous studies have demonstrated the antibacterial, antioxidant, and anti-inflammatory properties of pomegranate. The aim of the present study was to determine whether administration of pomegranate extract could result in morphometric changes in the kidneys of rats. Eighteen male rats (180-200 g) were divided into three groups that received either: G1, distilled water; G2, 250 mg kg-1 pomegranate extract; and G3, 500 mg kg-1 pomegranate extract via oral gavages daily for eight weeks. At the end of eight weeks, the rats were euthanized and their kidneys were removed and processed for morphometric analyses. In rats received pomegranate extract, the kidney weight, kidney weight/body weight ratio, cortex volume and glomerular volume were increased (p < 0.05), while, medulla volume and the number of glomeruli per kidney did not change. No pathological lesions were observed in the kidney. Therefore, pomegranate hydro-alcoholic extract at doses of 250 and 500 (mg kg-1) increased the volume of some parts of the kidney; however, it did not cause any pathological changes in the kidney.
Kidney
Pomegranate
Rat
Stereology
2016
03
01
1
6
https://vrf.iranjournals.ir/article_18999_b2a80f714d3b2c936efe01fa3bdb343b.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Inhibitory effect of Zataria multiflora Boiss. essential oil, alone and in combination with monolaurin, on Listeria monocytogenes
Mojtaba
Raeisi
Hossein
Tajik
Seyed Mehdi
Razavi Rohani
Bektas
Tepe
Hossein
Kiani
Rahem
Khoshbakht
Hesamaddin
Shirzad Aski
Hamed
Tadrisi
Listeria monocytogenes is one of the major causes of infections in developing countries. In this study, chemical composition and anti-listerial effect of the essential oil of Zataria multiflora Boiss. alone and in combination with monolaurin were evaluated at different pH values (5, 6, and 7) and temperatures (5 ˚C and 30 ˚C). Chemical composition of Zataria multiflora Boiss. essential oil was evaluated by gas chromatography-mass spectrometry (GC-MS) analysis. Minimum inhibitory concentration (MIC) of the essential oil and monolaurin were determined using microbroth dilution method and the interactions of essential oil and monolaurinwere determined by the evaluation of fractional inhibitory concentrations (FIC) index.Carvacrol (63.20%) and thymol (15.10%) were found as the main components of the essential oil. The MIC values of the oil and monolaurin at pH 7 and 30 ˚C were measured as 312.50 µg mL-1 and 125.00 µg mL-1, respectively. Combination of monolaurin and Z. multiflora essential oil were found to act synergistically (FIC index < 0.5) against L. monocytogenes under different pH and temperature conditions. Decrease in the pH and temperature values have increased the anti-listerial activity of monolaurin and the essential oil. The lowest MIC value of monolaurin and essential oil was observed at pH 5 and 5 ˚C. According to our results, the oil alone or in combination with monolaurin at low pH and temperature conditions showed a promising inhibitory effect on L. monocytogenes.
essential oil
Listeria monocytogenes
Minimum inhibitory concentration
Monolaurin
Zataria multiflora Boiss
2016
03
01
7
11
https://vrf.iranjournals.ir/article_19005_66fafbe65851bbf59a105e54211758ab.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Effects of propolis, royal jelly, honey and bee pollen on growth performance and immune system of Japanese quails
Sakine
Babaei
Shaeban
Rahimi
Mohammad Amir
Karimi Torshizi
Golamhosein
Tahmasebi
Seyed Naser
Khaleghi Miran
Effect of ethanolic extract of propolis, royal jelly, honey and bee pollen in comparison with virginiamycin (as growth promoter antibiotic) as regards the performance and immune system of Japanese quail were assessed. We used 256 mixed-sex quail chicks in a completely randomized design by eight treatments, including control, ethanolic extract of propolis 1000 (PE1) and 5000 (PE2) mg kg-1, pollen powder 1000 (PO1) and 5000 (PO2) mg kg-1, royal jelly 100 mg kg-1 (RJ), honey 22 g L-1 of drinking water (H) and virginiamycin 150 mg kg-1 (V), and four replicates of eight birds in each replication for 42 days. There was significant differences for weight gain (WG), feed intakes (FI) and feed conversion ratio (FCR) between experimental groups. In addition, WG in V treatment (18.82%), H and PO2 treatments (16.87%) and RJ treatment (12.00%) were significantly higher than to control group (p < 0.01). Significantly higher values of FCR were recorded in control group while group PE2 exhibited a significant decrease for 1 to 42 day (p < 0.05). The results of effect of bee products on antibodies titer showed an increase in theNewcastle disease (ND) titer when compared with control and virginiamycin groups (p < 0.01). There was significant difference in antibody production against avian influenza (AI) and sheep red blood cells (p < 0.01). Significant differences were observed in heterophils to lymphocytes ratio among PE1, PE2, PO1 and PO2 with V and control groups (p < 0.01).
Cell mediated immunity
Feed conversion
Honey bees products
Humoral immunity
2016
03
01
13
20
https://vrf.iranjournals.ir/article_19009_3cfe58f0e4c2d359a012f671e8656c23.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Evaluation of tendon healing using fibroblast like synoviocytes in rabbits: A biomechanical study
Mahboobeh
Azad-Tirgan
Farshid
Sarrafzadeh-Rezaei
Hassan
Malekinejad
Rahim
Hobbenaghi
Behnam
Heshmatian
Tendon never restores the complete biological and mechanical properties after healing. Several techniques are available for tissue-engineered biological augmentation for tendon healing like stem cells. Recently, synovium has been investigated as a source of cells for tissue engineering. In the present study, we investigated potentials of fibroblast like synoviocytes (FLSs) in tendon healing. Sixteen rabbits were divided randomly into control and treatment groups. One rabbit was used as a donor of synovial membrane (synovium). The injury model was unilateral complete transection through the middle one third of deep digital flexor tendon (DDFT). Subsequently, the tendon stumps were sutured with 3/0 nylon. In treatment group, 0.1 mL phosphate-buffered saline (PBS) solution containing 1 × 106 nucleated cells of FLSs was injected intratendinously at both tendon stumps just next to incision line. In control group, 0.1 mL PBS without FLSs was used for intratendinous injection. Model animals were euthanized at eight weeks, DDFTs were harvested and prepared for biomechanical study. Results of study showed that, there was no significant differences in biomechanical parameters values between FLSs treated and control groups. In conclusion, intratendinous injection of FLSs did not improve biomechanical properties during eight weeks in rabbit.
Biomechanical properties
Fibroblast like synoviocytes
Rabbit
Tendon
2016
03
01
21
26
https://vrf.iranjournals.ir/article_19010_b25b0ababdff3f06eb4cce6f36ce21f7.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Peroxisome proliferator-activated receptors (PPARα, PPARγ and PPARβ/δ) gene expression profile on ram spermatozoa and their relation to the sperm motility
Ali
Kadivar
Heidar
Heidari Khoei
Hossein
Hassanpour
Hamid
Ghanaei
Arefeh
Golestanfar
Hossein
Mehraban
Najmeh
Davoodian
Roohollah
Dehghani Tafti
Peroxisome proliferator-activated receptors (PPARs) are a member of nuclear receptors superfamily, which mainly regulate the expression of target genes involved in lipid and energy metabolism. These receptors are divided to three isotypes: PPARα, PPARγ and PPARβ/δ. Each isotype has a distinct tissue distribution relating to the distinct functions. In this study, the mRNA abundance for PPARα, PPARγ and PPARβ/δ was evaluated and compared with high and low motile ram spermatozoa. Semen samples from 6 adult rams were fractionated on a two layer discontinuous Percoll gradient to high and low motile sperm and quantitative parameters of sperm motility were determined by CASA. Total RNA was extracted and the mRNA abundance for each gene was measured by relative quantification technique with Real time PCR. The levels of three isotypes of PPAR transcripts were significantly higher in high motile semen samples using quantitative RT-PCR. Some of sperm motility indices were also significantly correlated with PPARα and PPARγ relative expression. This study revealed the novel association of PPAR gene isotypes with sperm motility. Data from our study suggested PPARs are one of the possible factors that can be studied in male infertility.
Gene expression
Peroxisome proliferator-activated receptors
Ram
Sperm motility
2016
03
01
27
34
https://vrf.iranjournals.ir/article_19011_0d55d91283b4e979c16fb1d8a7dd8bfe.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Measure of energy related biochemical metabolites changes during peri-partum period in Makouei breed sheep
Vahid
Mohammadi
Ehsan
Anassori
Shoja
Jafari
Makouei sheep is one of the famous breeds in Iran which is reared in Azerbaijan province for their meat, milk and wool. Fifty clinically healthy Makouei ewes were selected to study the variations in energy-related blood metabolites during peri-partum period. Blood was collected from Jugular vein from each sheep on day 7 before the expected lambing time, day of parturition and also day 7 postpartum to determine total protein, albumin, urea, cholesterol, glucose, triglyceride, β-hydroxybutyrate (BHB) and non- esterified fatty acid (NEFA) levels. Serum total protein and albumin concentrations were gradually decreased during pre-partum period and reached the lowest level after parturition (p > 0.05). Blood urea concentration was significantly decreased to the lowest level at parturition (p < 0.05). Serum cholesterol and triglycerides were gradually decreased and reached low levels after lambing (p < 0.05). Serum glucose concentrations were significantly lower at pre-partum period than post-partum (p < 0.05). The serum NEFA and BHB concentrations were higher before lambing and thereafter decreased (p < 0.05). Current findings regarding the blood parameters may expand our knowledge for the diagnosis and prognosis of reproductive and metabolic diseases in Makouei sheep during these phases.
Beta-hydroxybutyrate
Blood metabolites
Makouei sheep
Non- esterified fatty acids
2016
03
01
35
39
https://vrf.iranjournals.ir/article_19036_3265fd4e8b7ef3486b5a83a7cc2e4d9b.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Developmental study of rectum in broiler chicken: A stereological and morphometrical study
Rahmat-Allah
Fatahian Dehkordi
Poria
Ghahremani
The objective of this study was to investigate development of the weight and the morphological development of the rectum in broiler chickens. Twenty broiler chickens (Ross 308) were used in this experiment and they were 12, 20, 35 and 44 days of age. Samples from the rectum of chicks were sectioned in an unbiased manner and examined quantitatively using stereology. In addition, the weight of both body (BW) and rectum and also rectum weight as a proportion of BW, height and width of the villi and thickness of rectum wall were measured. The results revealed that the body and rectum weight were increased with age. The greatest rectum weight as a proportion of BW was observed on day 20. An increase in height and width of the villi during the study period were obtained. The increase was more significant on days 35 and 44 than on days 12 and 20 (p < 0.05). There were significant difference in thickness of tunica mucosa, submucosa and muscular layer of the rectum on day 20 compared to day 35 (p < 0.05). The increase in the volume density of the wall, tunica mucosa and tunica muscular was greater on day 20 than on day 35. However, significant differences were observed in volume density of this layers between days 35 and 44 compared to days 12 and 20 (p < 0.05).
Broiler chicken
Developmental period
Morphometrical study
Rectum
2016
03
01
41
45
https://vrf.iranjournals.ir/article_19037_aa9e907db4d1a4abf9f78dfd62f9ad17.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Effects of histidine and vitamin C on isoproterenol-induced acute myocardial infarction in rats
Masoumeh
Moradi-Arzeloo
Amir Abbas
Farshid
Esmaeal
Tamaddonfard
Siamak
Asri-Rezaei
In the present study, we investigated the effects of histidine and vitamin C (alone or in combination) treatments against isoproterenol (a β-adrenergic receptor agonist)-induced acute myocardial infarction in rats. We used propranolol (a β-adrenergic receptor blocker) to compare the results. Rats were given intraperitoneal injections of histidine (40 mg kg-1) and vitamin C (40 mg kg-1) alone and combined daily for 21 days. Propranolol (10 mg kg-1) was orally administered daily for 10 days (from day 11 to day 21). Myocardial infarction was induced by subcutaneous injections of 150 mg kg-1 of isoproterenol at an interval of 24 hr on days 20 and 21. Blood and tissue samples were taken for histopathological and biochemical evaluations following electrocardiography recording on day 21. Isoproterenol elevated ST segment, increased heart weight, heart rate, serum activities of aspartate transaminase, lactate dehydrogenase, creatine kinase-MB and heart tissue content of malondialdehyde, and decreased R wave amplitude and superoxide dismutase and catalase activities of heart tissue. Necrosis, edema and inflammatory cells infiltration were observed in myocardial tissue sections. Our results indicated that histidine and vitamin C alone, and especially in combination prevent isoproterenol-induced cardiotoxicity and have similar protective effects with propranolol. Cardioprotective effects of histidine and vitamin C may be associated with their ability to reduce free radical-induced toxic effects.
Histidine
Isoproterenol
Myocardial infarction
Propranolol
Vitamin C
2016
03
01
47
54
https://vrf.iranjournals.ir/article_19038_8f0cc873bcb6b9d7a42f5c6014addfca.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
A study of visceral leishmaniasis in owned dogs with dermal lesions in Mashhad area, Khorasan Razavi province
Sadaf
Sabzevari
Gholamreza
Razmi
Abolghasem
Naghibi
Javad
Khoshnegah
Dog is the main reservoir host of visceral leishmaniasis in Iran. The present study was carried out to investigate visceral leishmaniasis in owned dogs with dermal lesions in Mashhad, Khorasan Razavi province. Thirty- nine owned dogs with dermal lesions were selected. During study, four of dogs were euthanized. The dermal smears, blood and tissue samples were collected and examined using parasitological, serological and molecular methods. A total of 39 examined dogs, leishman bodies were microscopically detected in 33.30% (13/39) of dermal smears. The sera samples were tested by indirect immunofluorescent antibody test (IFAT). Antibody against Leishmania infantum was detected in 26.00% (10/39) dogs. According to semi-nested PCR, DNA of Leishmania infantum was detected in 2.50% (1/39) of blood samples and in 75.00 % (3/4) of different tissues of euthanized dogs. BLAST analysis of the sequenced samples indicated a 99.00% similarity with kDNA of Leishmania infantum. Based on the results, it is concluded that visceral leishmaniasis due to L. infantum is distributed among household dogs of this area and it needs more surveillance to control the disease by public health and veterinary authorities.
Dog
Iran
Leishmania infantum
Mashhad
2016
03
01
55
61
https://vrf.iranjournals.ir/article_19039_dffd492d3e263d1c6d7e09d8b718e186.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Protective effect of ethyl pyruvate on mice sperm parameters in phenylhydrazine induced hemolytic anemia
Ali Akbar
Mozafari
Rasoul
Shahrooz
Abbas
Ahmadi
Hassan
Malekinjad
Karim
Mardani
The aim of the present study was to assess the protective effect of ethyl pyruvate (EP) on sperm quality parameters, testosterone level and malondialdehyde (MDA) in phenylhydrazine (PHZ) treated mice. For this purpose, 32 NMRI mice with the age range of 8 to 10 weeks, weight average 26.0 ± 2.0 g, were randomly divided into four equal groups. The control group (1) received normal saline (0. 1 mL per day) by intraperitoneal injection (IP). Group 2 (PHZ group) was treated with initial dose of PHZ (8 mg 100 g-1, IP) followed by 6 mg 100 g-1 , IP every 48 hr. Group 3, (Group PHZ+EP) received PHZ (according to the previous prescription) with EP (40 mg kg-1, daily, IP). Ethyl pyruvate group (4) received only EP (40 mg kg-1, daily, IP). Treatment period was 35 days. After euthanasia, sperms from caudal region of epididymis were collected and the total mean sperm count, sperm viability, motility and morphology were determined. Testis tissue MDA and serum testosterone levels of all experimental groups were also evaluated. A considerable reduction in mean percentage of number, natural morphology of sperm, sperm motility and viability and serum testosterone concentration besides DNA injury increment among mice treating with PHZ in comparison with control group were observed. However, in PHZ+EP group the above mentioned parameters were improved. This study showed that PHZ caused induction of toxicity on sperm parameters and reduction of testosterone as well as the increment of MDA level and EP as an antioxidant could reduce destructive effects of PHZ on sperm parameters, testosterone level and lipid peroxidation.
Ethyl pyruvate
Mouse
Phenylhydrazine
Sperm parameters
Stress oxidative
2016
03
01
63
68
https://vrf.iranjournals.ir/article_19040_476a3161c1d0915b5712771e042392be.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Molecular identification and phylogenic analysis of Bartonella henselae isolated from Iranian cats based on gltA gene
Ramin
Mazaheri Nezhad Fard
Seyed Milad
Vahedi
Iraj
Ashrafi
Faranak
Alipour
Golnaz
Sharafi
Hesam
Akbarein
Seyed Javid
Aldavood
One of the most important species of the Bartonella genus is B. henselae that causes a zoonotic infection, cat scratch disease (CSD). The main source of the bacteria is cat and the carrier is Ctenocephalides felis flea. One hundred and forty nail and saliva samples were collected from 70 domestic cats. Positive samples for B. henselae were characterized by polymerase chain reaction (PCR) and sequencing. Sequences of gltA gene were trimmed using BioEdit software and then compared with the sequences of the same gene from B. henselae isolated from cats and humans in GenBank database. Phylogenic tree was constructed using CLC Sequence Viewer software and unweighted pair group method with arithmetic mean (UPGMA) method. Molecular assessments showed that five samples out of 70 nail samples (7.14%) and one sample out of 70 saliva samples (1.42%) were genetically positive for B. henselae. At least an 87.00% similarity was seen between the gene sequences from the current study and the reference sequences from the GenBank database. Phylogenic analysis has shown that strains isolated in this study were grouped in a different haplo group, compared to other strains.Among the Asian countries, the prevalence of the bacteria in Iran was close to that in Japan and Turkey. In conclusion, findings of this study showed the prevalence of B. henselae in Iranian cats which is important due to its public health issues, especially for the immunocompromised pet owners.
Bartonella henselae
Cat
Iran
Zoonosis
2016
03
01
69
72
https://vrf.iranjournals.ir/article_19041_456e3c640c4bcc04230860f43705f4e9.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Parasitic infection in various stages life of cultured Acipenser persicus
Milad
Adel
Reza
Safari
Zahra
Yaghoubzadeh
Hassan
Fazli
Elham
Khalili
The present study was conducted to evaluate the status of the parasite fauna in Acipenser persicus at different development stages, in order to find prevention protocols for parasitic diseases in this valuable species. For this purpose, sampling from each sex breeder, 10 egg samples, 5-day-old larvae (n = 20), 20-day-old larvae (n = 80) and fingerling of A. persicus (n = 60) released in earthen ponds were done. After the bioassay and preparing wet mount from the internal and external organs, identification was done according to the keys. According to the results, no fauna parasites were isolated from egg samples and 5-day-old larvae; but Trichodina spp. was isolated from 20-day-old larvae. Also, the same protozoan was isolated from fingerling released in earthen ponds, the mean intensity, prevalence and range of contamination by fingerling were higher with compared to 20-day-old larvae. Trichodina sp. and Diplostomum spathaceum were isolated from skin and eyes of females, respectively. However, Trichodina sp. and Ichthyophthirius multifiliis were isolated from skin of male breeders. In this study, no parasites were isolated from internal organs of larves and fingerling but four intestinal parasites included: Cucullanus sphaerocephlaus, Anisakis sp., Skyrjabinopsilus semiarmatus, and Lepto-rhynchoides plagicephalu were isolated from internal organs of breeder. Based on a wide range of parasitic infection observed in various life stages of A. persicus, it seems necessary to consider hygienic and management measures.
Acipenser persicus
Iran
parasite
2016
03
01
73
77
https://vrf.iranjournals.ir/article_19044_715bdfd05bb0e038de103f6af342a40f.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Large B-cell lymphoma in a dog: A cyto-histopathological evaluation and Immunophenotyping according to WHO classification for canine lymphomas
Zahra
Nikousefat
Mohammad
Hashemnia
Moosa
Javdani
In the present study, we described cyto-histopathological features and immunophenotyping of the large B-cell lymphoma in an 8-year-old mixed breed dog with applying the World Health Organization (WHO) system of classification of canine lymphomas. In fine-needle aspiration (FNA), lymph nodes were involved by neoplastic cells of intermediate to large size with deep blue cytoplasm; consist of centroblasts, immunoblast and medium-sized cells. Histopathologically, the follicles and sinuses of lymph nodes were replaced by sheets of numerous immunoblasts (less than 90.0% of total cells) and centroblasts. Numerous mitotic figures were also observed. Immunohistochemical analysis presented that the neoplastic cells express B-cell phenotype CD20 and CD79a, but do not stain for T phenotype CD3. On the basis of cytology, histopathology and immunohistochemical findings, the present tumor was diagnosed as diffuse large B-cell lymphoma, high-grade centroblastic type (DLBCL-CB) according to WHO histological classification. Applying this classification system for diagnosis of canine lymphomas is very useful and has a high accuracy and consistency. However, further co-operative studies between clinicians and pathologists should be performed, in order to improve the effectiveness of this classification.
Canine lymphomas
Cyto-histopathological features
Immunohistochemistry
2016
03
01
79
83
https://vrf.iranjournals.ir/article_19049_a829fe45b171c7b1f4380aee26ab1dbe.pdf
Veterinary Research Forum
Vet Res Forum
2008-8140
2008-8140
2016
7
1
Patent ductus arteriosus in a lamb: A case report
Afshin
Jafari Dehkordi
Farzaneh
Hoseini
Patent ductus arteriosus (PDA) is a persistent patency of a vessel normally present in the fetus that connects the pulmonary arterial system to the aorta. The ductus arteriosus fails to close at birth when breathing commences and placental blood circulation is removed. Closure of the ductus arteriosus arises in response to decline pulmonary vascular resistance and increased systemic vascular resistance. This report describes a case of PDA in a two-month-old male lamb with clinical signs of machinery murmur, tachycardia, increase respiratory rate, weakness and ill thrift. Echocardiographic examination and necropsy finding confirmed PDA.
Echocardiography
Lamb
Necropsy
Patent ductus arteriosus
2016
03
01
85
88
https://vrf.iranjournals.ir/article_19051_4ce59e23746c8c250906d8653147fed3.pdf