Detection and molecular characterization of infectious bronchitis virus from ‎recent outbreaks in broiler flocks in Sulaimani Governorate

Document Type : Short Communication

Authors

1 Department of Clinic and Internal Medicine, College of Veterinary Medicine, University of Sulaimani, Sulaymaniyah, Iraq

2 Veterinary Laboratory, PCR Unit, Directorate of Veterinary, Sulaimani, Sulaymaniyah, Iraq‎

Abstract

Infectious Bronchitis (IB) is one of the most important viral diseases of poultry. It causes major financial losses in the poultry industry. It is caused by the infectious bronchitis virus (IBV). This study investigated the genetic origin and diversity of IBV by analyzing the partial S1 sequence derived from local broiler farms in different regions of Sulaimani Governorate. Detection was performed using a newly designed primer for the partial S1 gene. The present was undertaken to understand the molecular relationship between the strains across the world compared with field virus isolates by phylogenetic tree and sequences-analysis. Phylogenetic tree demonstrated that field isolate made a novel group (sul-5/17-like strain), indicating new variants. Also, field virus sequences were

Keywords


  1. Butcher GD, Shapiro DP, Miles RD. Infectious bronchitis virus: Classical and variant strains. One of a series of the Veterinary medicine-large animal clinical sciences department, Florida cooperative extension service. UF/IFAS Extension 2011; VM127.
  2. Hafez HM. Avian adenoviruses infections with special attention to inclusion body hepatitis/hydropericardium syndrome and egg drop syndrome. Pak Vet J 2011; 31(2): 85-92.
  3. Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res 2007; 38(2):281-297.
  4. Su JL, Zhou ZT. Guo ZS, et al. Identification of five bronchitis virus (IBV) strains isolated in China and phylogenetic analysis of the S1 gene. Afri J Microbiol Res 2012; 6(9):2194-2201.
  5. Haqshenas G, Assasi K, Akrami H. Isolation and molecular characterization of infectious bronchitis virus, isolate Shiraz3. IBV, by RT-PCR and restriction enzyme analysis. Iran J Vet Res 2005; 6(2):9-15.
  6. Poorbaghi SL, Mohammadi A, Asasi K. Molecular detection of avian infectious bronchitis virus serotypes from clinically suspected broiler chicken flocks in Fars province of Iran. Pak Vet J 2012; 32(1):93-97.
  7. Terregino C, Toffan A, Serena Beato M, et al. Pathogenicity of a QX strain of infectious bronchitis virus in specific pathogen free and commercial broiler chickens, and evaluation of protection induced by a vaccination programme based on the Ma5 and 4/91 serotypes. Avian Pathol 2008; 37(5):487-493.
  8. Balestrin E, Fraga AP, Ikuta N, et al. Infectious bronchitis virus in different avian physiological systems- a field study in Brazilian poultry flocks. Poult Sci 2014; 93(8):1922-1929.
  9. Boltz DA, Nakai M, Bahra JM. Avian infectious bronchitis virus: a possible cause of reduced fertility in the rooster. Avian Dis 2004; 48(4):909-915.
  10. Crinion RA, Hofstad MS. Pathogenicity of four serotypes of avian infectious bronchitis virus for the oviduct of young chickens of various ages. Avian Dis 1972; 16(2):351-363.
  11. Pohuang T, Chansiripornchai N, Tawatsin A, et al. Detection and molecular characterization of infectious bronchitis virus isolated from recent outbreaks in broiler flocks in Thailand. J Vet Sci 2009; 10(3):219-223.
  12. Boroomand Z, Asasi K, Mohammadi A. Pathogenesis and tissue distribution of avian infectious bronchitis virus isolate IRFIBV32 (793/B serotype) in experimentally infected broiler chickens. Sci World J 2012;403537 doi:10.1100/2012/402537
  13. Zhu JG, Qian HD, Zhang YL, Hua XG, Wu ZL. Analysis of similarity of the SI gene in infectious bronchitis virus (IBV) isolates in Shanghai, China. Arch Med Vet 2007;39(3):223-8.
  14. Zwaagstra, KA, van der Zeijst, BA, Kusters, JG. Rapid detection and identification of avian infectious bronchitis virus. J Clin Microbiol 1992; 30(1):79-84.
  15. Cavanagh D. Severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus. Avian Pathol 2003: 32(6):567-582.
  16. Tamura K, Stecher G, Peterson D, et al. MEGA6: Molecular evolutionary genetics analysis version 6.0. Mol Bio Evol 2013; 30(12):2725-2729
  17. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22(22):4673-80.
  18. Rauf HS. Investigation and molecular characterization of avian infectious bronchitis virus in suspected broiler farms in Slemani Governorate. MS Thesis, University of Sulaimani. Sulaymaniyah, Iraq: 2014.
  19. de Wit JJ, Nieuwenhuisen-van Wilgen J, Hoogkamer A, et al. Induction of cystic oviducts and protection against early challenge with infectious bronchitis virus serotype D388 (genotype QX) by maternally derived antibodies and by early vaccination. Avian Pathol 2011; 40(5):463-471.
  20. Mahmood ZH, Sleman RR, Uthman AU. Isolation and molecular characterization of Sul/01/09 avian infectious bronchitis virus, indicates the emergence of a new genotype in the Middle East. Vet Microbiol 2011; 150(1):21-7.