Study of growth kinetic and gastrointestinal stability of acid-bile resistant Lactobacillus plantarum strains isolated from Siahmazgi traditional cheese

Document Type : Original Article


Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


In this study, 22 Lactobacillus plantarum strains isolated from Siahmazgi traditional cheese were evaluated using different tests including resistance to low pH (1.50 and 2.50) and bile salt (0.50 and 1.00%), growth kinetic at low pH values and survival under simulated gastric and intestinal fluids. All the strains retained their viability at pH 2.50. However, the survival of all of the isolates was decreased at pH 1.50. Ten out of 22 strains which were able to tolerate low pH were selected for further investigations. All the selected isolates were able to grow at low pH. Strain F2 showed the highest specific growth rate. Five out of 10 isolates showed a significant decrease in bacterial count varied from 2.00 to 7.00 log CFU mL-1 during 3 hr exposure to 0.50% bile salt, while five isolates represented resistance to 0.50% bile during 3 hr. A significant reduction was observed in survival of all of the isolate at 1.00% bile salt concentration. Furthermore, viability of the selected isolates was lowered during 1 hr incubation under gastric conditions, while it remained unchanged within next 2 hr. Although, no significant changes were seen in bacterial count of the selected isolates during 1 hr of exposure to simulated intestinal condition, the survival of the isolates was relatively reduced after 3 hr. In conclusion, five out of 22 examined L. plantarum isolates showed appropriate resistance properties, therefore, could be good candidates for further examinations including functional and safety evaluation supporting their use as probiotics.


  1. Bove P, Gallone A, Russo P, et al. Probiotic features of Lactobacillus plantarum mutant strains. Appl Microbiol Biotechnol 2012; 96(2): 431-441.
  2. Fernández MF, Boris S, Barbés C. Safety evaluation of Lactobacillus delbrueckii subsp. lactis UO 004, a pro-biotic bacterium. Res Microbiol2005;156(2): 154-160.
  3. Argyri AA, Zoumpopoulou G, Karatzas KAG, et al. Selection of potential probiotic lactic acid bacteria from fermented olives by in vitro tests. Food Microbiol 2013; 33(2): 282-291.
  4. Turchi B, Mancini S, Fratini F, et al. Preliminary evaluation of probiotic potential of Lactobacillus plantarum strains isolated from Italian food products. World J Microbiol Biotechnol 2013; 29(10): 1913-1922.
  5. Ayeni FA, Sánchez B, Adeniyi BA, et al. Evaluation of the functional potential of Weissella and Lactobacillus isolates obtained from Nigerian traditional fermented foods and cow's intestine. Int J Food Microbiol 2011; 147(2): 97-104.
  6. Ruiz-Moyano S, Martín A, Benito MJ, et al. Screening of lactic acid bacteria and Bifidobacteria for potential probiotic use in Iberian dry fermented sausages. Meat Sci 2008; 80(3): 715-721.
  7. Arokiyaraj S, Islam VIH, Bharanidharan R, et al. Antibacterial, anti-inflammatory and probiotic potential of Enterococcus hirae isolated from the rumen of Bos primigenius.World J Microbiol Biotechnol2014;30(7): 2111-2118.
  8. Archer AC, Halami PM. Probiotic attributes of Lactobacillus fermentum isolated from human feces and dairy products. Appl Microbiol Biotechnol2015; 99(19): 8113-8123.
  9. Angmo K, Kumari A, Savitri, et al. Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT- Food Sci Technol2016; 66: 428-435.
  10. Verdenelli MC, Ghelfi F, Silvi S, et al. Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces. Eur J Nut 2009; 48(6): 355-363.
  11. Partovi R, Gandomi H, Akhondzadeh Basti A, et al. Microbiological and chemical properties of Siahmazgi cheese, an Iranian artisanal cheese: isolation and identification of dominant lactic acid bacteria. J Food Process Preserv2014;39(6): 871-880.
  12. Lähteinen T, Malinen E, Koort JMK, et al. Probiotic properties of Lactobacillus isolates originating from porcine intestine and feces. Anaerobe 2010; 16(3): 293-300.
  13. McDonald LC, Shieh DH, Fleming HP, et al. Evaluation of malolactic-deficient strains of Lactobacillus plantarum for use in cucumber fermentations. Food Microbiol 1993; 10(6): 489-499.
  14. Gao Y, Li D, Liu S, et al. Probiotic potential of L. sake C2 isolated from traditional Chinese fermented cabbage. Eur Food Res Technol 2012; 234: 45-51.
  15. Zhang Y, Zhang L, Du M, et al. Antimicrobial activity against Shigella sonnei and probiotic properties of wild lactobacilli from fermented food. Microbiol Res 2011; 167(1): 27-31.
  16. Plessas S, Nouska C, Karapetsas A, et al. Isolation, characterization and evaluation of the probiotic potential of a novel Lactobacillus strain isolated from Feta-type cheese. Food Chem2017;226: 102-108.
  17. Turková K, Mavrič A, Narat M, et al. Evaluation of Lactobacillus strains for selected probiotic properties. Folia Microbiol (Praha) 2015; 58(4): 261-267.
  18. Fang Z, Hongfei Z, Junyu Z, et al. Evaluation of probiotic properties of Lactobacillus strains isolated from traditional Chinese cheese. Ann Microbiol 2015; 65: 1419-1426.
  19. Arasu MV, Al-Dhabi NA, Rejiniemon TS, et al. Identification and characterization of Lactobacillus brevis P68 with antifungal, antioxidant and probiotic functional properties. Indian J Microbiol2015;55: 19-28.
  20. Park YU, Kim MD, Jung DH, et al. Probiotic properties of lactic acid bacteria isolated from Korean rice wine Makgeolli. Food Sci Biotechnol2015; 24: 1761-1766.
  21. Oguntoyinbo FA, Narbad A. Multifunctional properties of Lactobacillus plantarum strains isolated from fermented cereal foods. J Funct Foods 2015; 17: 621-631.
  22. Wu C, Zhang J, Wang M, et al. Lactobacillus casei combats acid stress by maintaining cell membrane functionality. J Ind Microbiol Biotechnol 2012; 39: 1031-1039.
  23. Huang HY, Hsieh HY, King VAE, et al. To pre-challenge lactic acid bacteria with simulated gastrointestinal conditions is a suitable approach to studying potential probiotic properties. J Microbiol Methods 2014; 107: 138-146.
  24. Owusu-Kwarteng J, Tano-Debrah K, Akabanda F, et al. Technological properties and probiotic potential of Lactobacillus fermentum strains isolated from West African fermented millet dough. BMC Microbiol2015;15: 261. doi: 10.1186/s12866-015-0602-6
  25. Das P, Khowala S, Biswas S. In vitro probiotic characterization of Lactobacillus casei isolated from marine samples. LWT- Food Sci Technol2016; 73: 383-390.
  26. Lee YK, Salminen S. The coming of age of probiotics. Trends Food Sci Technol 1995; 6(7): 241-245.