Identification of bovine leukemia virus in raw milk samples in North-West of Iran

Document Type : Original Article

Authors

1 Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

2 Department of Social Medicine, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

3 Department of Food science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran

4 Department of Laboratory Sciences, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran

5 Hematology and Oncology Research Center, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Bovine leukemiavirus (BLV) is one of the most important carcinogenic viruses genetically related to the human T-cell lymphotropic viruses (HTLV-1 and HTLV-2). The virus infects type B lymphocytes and creates lymph glands tumors. Recently, the association between the presence of this virus and breast cancer has been addressed in humans. Here, we studied the prevalence of BLV in the samples of raw milk of native Iranian and Iranian-foreign cows in traditional, semi-industrial and industrial dairy farms in rural and urban areas of Zanjan province. Raw milk samples of cows were collected manually in sterile tubes. The samples were tested by nested-PCR method. Forty samples (9.93%) out of 403 samples showed BLV contamination. In this study, nested-PCR was successfully applied to determine the level of contamination in raw milk samples from cows infected with BLV. Furthermore, a relatively high rate of BLV infection was found in dairy cows in Zanjan province, northwestern of Iran.

Keywords


  1. Jaworski JP, Porta NG, Gutierrez G, et al. Short communication: Relationship between the level of bovine leukemia virus antibody and provirus in blood and milk of cows from a naturally infected herd. J Dairy Sci 2016; 99(7): 5629-5634
  2. Nikbakht Broujeni Gh, Emam SM, Rezaei H. Detection of bovine leukemia virus infection by nested-PCR analysis [Persian]. Vet J (Pajouhesh-VA- Sazandegi) 2011; 24(2): 1-8.
  3. González ET, Norimine J, Valera AR, et al. A rapid and sensitive diagnosis of bovine leukaemia virus infection using the nested shuttle polymerase chain reaction. Pesq Vet Bras 1999;19(2): 63-67.
  4. Malovrh T, Pate M, Ocepek M, et al. Comparison of agar gel immunodiffusion test, enzyme-linked immune-sorbent assay and PCR in diagnostics of enzootic bovine leukosis. Vet Glas 2005;59(3-4):363-370.
  5. Yang Y, Fan W, Mao Y, et al. Bovine leukemia virus infection in cattle of China: association with reduced milk production and increased somatic cell score. J Dairy Sci 2016;99(5):3688-3697.
  6. Ohira K, Nakahara A, Konnai S, et al. Bovine leukemia virus reduces anti‐viral cytokine activities and NK cyto-toxicity by inducing TGF‐β secretion from regulatory T cells. Immun Inflamm Dis 2016;4(1):52-63.
  7. Naif HM, Daniel RC, Cougle WG, et al. Early detection of bovine leukemia virus by using an enzyme-linked assay for polymerase chain reaction-amplified proviral DNA in experimentally infected cattle. J Clin Microbiol 1992;30(3):675-679.
  8. Polat M, Takeshima SN, Hosomichi K, et al. A new genotype of bovine leukemia virus in South America identified by NGS-based whole genome sequencing and molecular evolutionary genetic analysis. Retrovirology 2016;13:4. doi: 10.1186/s12977-016-0239-z.
  9. Zaher KS, Ahmed WM. Bovine leukemia virus infection in dairy cows in Egypt. Acad J Cancer Res 2014; 7(2):126-130.
  10. Nishimori A, Konnai S, Okagawa T, et al. Identification of an atypical enzootic bovine leukosis in Japan by using a novel classification of bovine leukemia based on immunophenotypic analysis. Clin Vaccine Immunol 2017;24(9): e00067-17. doi: 10.1128/CVI.00067-17.
  11. Escalera‐Zamudio M, Greenwood AD. On the classification and evolution of endogenous retrovirus: human endogenous retroviruses may not be human after all. APMIS 2016;124(1-2):44-51.
  12. Kale M, Hasircioğlu S, Yavru S, et al. Bovine leukemia virus antibodies in dairy cattle farms and milk cooling
    tanks. Acta Sci Vet 2014;42: 1-9.
  13. Van de Perre P, Lepage P, Homsy J, et al. Mother-to-infant transmission of human immunodeficiency virus by breast milk: presumed innocent or presumed guilty? Clin Infect Dis 1992;15(3):502-507.
  14. Sellers TA, Vierkant RA, Djeu J, et al. Unpasteurized milk consumption and subsequent risk of cancer. Cancer Causes Control 2008;19(8):805-811.
  15. Pourthamasbi Bilesvar V. Medical and molecular virology [Persian]. Tehran, Iran: Ebne-Sina Publication 2013; 317-340.
  16. Polat M, Takeshima SN, Aida Y. Epidemiology and genetic diversity of bovine leukemia virus. Virol J 2017;14(1):209. doi: 10.1186/s12985-017-0876-4.
  17. Lutz H, Hunsmann G, Schüpbach J. Transmission and Epidemiology. In: Kurth R, Bannert N (Eds). Retroviruses: molecular biology, genomics and pathogenesis. Norfolk, UK: Caister Academic Press 2010; 217.
  18. Miller JM, van der Maaten MJ. Bovine leukosis- -its importance to the dairy industry in the United States. J Dairy Sci 1982;65(11):2194-2203.
  19. Willems L, Portetelle D, Kerkhofs P, et al. In vivo transfection of bovine leukemia provirus into sheep. Virology 1992;189(2):775-777.
  20. Barez PY, de Brogniez A, Carpentier A, et al. Recent advances in BLV research. Viruses 2015;7(11): 6080-6088.
  21. Buehring GC, Shen HM, Jensen HM, et al. Exposure to bovine leukemia virus is associated with breast cancer: a case-control study. PLoS One 2015; 10(9): e0134304. doi: 10.1371/journal.pone.0134304.
  22. Nekoei S, Taktaz Hafshejani T, Doosti A, et al. Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep. Pol J Vet Sci 2015;18(4):703-707.
  23. Mousavi S, Haghparast A, Mohammadi G, et al. Prevalence of bovine leukemia virus (BLV) infection in the northeast of Iran. Vet Res Forum 2014; 5(2): 135-139.
  24. Giovanna M, Carlos UJ, María UA, et al. Bovine leukemia virus gene segment detected in human breast tissue. Open J Med Microbiol 2013;3(1):84-90.
  25. Lee J, Kim Y, Kang CS, et al. Investigation of the bovine leukemia virus proviral DNA in human leukemias and lung cancers in Korea. J Korean Med Sci 2005;20(4):603-606.