Seasonal influence on serum kisspeptin level and its association with hormonal levels and semen kinematics of Buffalo bulls (Bubalus bubalis)

Document Type : Original Article

Authors

1 Department of Physiology, Faculty of Biosciences, University of Veterinary and Animal Sciences Lahore, Pakistan

2 Department of Physiology, Faculty of Biosciences, University of Veterinary and Animal Sciences Lahore

3 Animal Sciences Institute, National Agricultural Research Centre, Islamabad, Pakistan

4 Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore, Pakistan

5 Department of Physiology, Faculty of Biosciences, UNiversity of Veterinary and Animal Science, Lahore, Pakistan

6 Department of Physiology, Faculty of Bioscience, University of Veterinary and Animal Science Lahore, Pakistan

7 Department of Physiology, Faculty of Bioscience, University of Veterinary and animal Sciences, outfall road Lahore

8 Department of Physiolgoy, Faculty of Bioscience, University of Veterinary and Animal Sciences Lahore, Pakistan

10.30466/vrf.2020.122142.2873

Abstract

Kisspeptin has an important role in the stimulation of hypothalamic-pituitary gonadal axis in term of pubertal development, release of reproductive and metabolic hormone and ultimately affecting the fertility. The aim of the present study was to evaluate the serum kisspeptin level and its correlation with semen quality and selected hormones in buffalo bulls during the summer and spring seasons. Semen and blood samples from eight Nili-Ravi buffalo bulls (age 9.21 ± 1.02 years) were collected. Semen was analysed using computer-assisted semen analysis (CASA). Serum concentrations of kisspeptin, gonadotropin releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, triiodothyronine (T3), thyroxin (T4) and insulin like growth factor (IGF-1) were estimated using ELISA kits. Kisspeptin was neither affected by seasons and nor correlated with semen parameters and hormones. Higher levels of GnRH, LH, cortisol, IGF-1, total motility (TM), average path velocity (VAP), straight-line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN) were recorded in summer than spring. Correlation of GnRH vs. IGF-1 and LH, LH vs. IGF-1 and cortisol, FSH vs. T4 and testosterone, testosterone vs. T3 and T4, T3 vs. T4 were observed. The GnRH and IGF-1 were positively associated with TM, VAP, VSL, VCL and LIN. LH was correlated with VSL, STR and LIN. In conclusion, GnRH, LH and IGF-1 correlation with semen parameters can be used to indicate quality of semen. The buffalo bulls are well adapted and can give quality semen in the summer season.

Keywords



Articles in Press, Accepted Manuscript
Available Online from 15 June 2021
  • Receive Date: 25 February 2020
  • Revise Date: 11 May 2020
  • Accept Date: 06 July 2020