The suppression of the malignancy of mammary tumor in mice model by inactivated preparation of Mycobacterium obuense

Document Type : Original Article

Authors

1 Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

2 aDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

3 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

4 Immunology Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

5 Department of Statics and Epidemiology, Tabriz University of Medical Sciences, Tabriz, Iran

6 Faculty of Vet.Med., Tabriz university

10.30466/vrf.2021.525359.3144

Abstract

Breast cancer (BC) is a significant cause of global mortality in women. This study aimed to evaluate the immune-activation of malignant BC via the administration of attenuated Mycobacterium obuense (M. obuense). For this purpose, an in vivo model was developed with BALB/c mice. Mice were injected with 2×106 4T1 cells with breast tumor cell line. Forty-two mice were equally divided into control as well as low dose (0.2 mg/100 µl) and high dose (0.5 mg/100µl) groups of M. obuense to investigate gene expression in the antitumor effects of M. obuense. In one group, paclitaxel was administrated as a choice drug in BC treatment. Antitumor manners were characterized by cytotoxicity against tumor target cells (MTT), size of the tumor, the expression of some BC metastatic genes together with pathology. MTT assay demonstrated that different concentrations of both low and a high doses of bacteria did present no cytotoxicity effect on 4T1 cells. According to our findings, M. obuense significantly repressed tumor growth. M. obuense downregulated the expression of collagen type I alpha 1 (COLIA1), cFos, alkaline phosphatase (ALP), claudin 3 (cldn3), and conversely, activated transcription factor 4 (ATF4), and Twist related protein-1 (Twist1). All these alternations induced a decrease in the migratory and invasive capabilities of BC. The result of pathology is indicative of tumor regression in the paclitaxel and HK- M. obuense -recipient group .Thus, it seems highly likely that M. obuense may impinge upon cell growth and metastatic behavior of malignant cells exerting anti-tumor activity in BC.

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Articles in Press, Accepted Manuscript
Available Online from 08 May 2021
  • Receive Date: 24 February 2021
  • Revise Date: 24 April 2021
  • Accept Date: 08 May 2021