Comparative consequences of two sources of aflatoxins in ducklings experimental aflatoxicosis

Document Type : Original Article

Authors

1 Department of Poultry Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

2 Tarbiat Modares University Department of Poultry Science

10.30466/vrf.2020.104547.2488

Abstract

The purpose of this study was to investigate the effect of two aflatoxins (AFs) sources for experimental induction of aflatoxicosis in ducklings. Dietary supplementation of aflatoxin-contaminated grounded rice grains previously was cultured by Aspergillus parasiticus and dietary supplementation of aflatoxin methanolic extract of contaminated rice grains. A total of 18 four-day-old ducklings were treated with varying sources of AFs. Treatments included: A: Control (basal diet without AFs), B: Contaminated feed with 0.2 mg kg-1 AFs (ground rice grains), C: Contaminated feed with 0.2 mg kg-1 AFs (methanol extract of contaminated rice grains). Results showed a significant decrease in serum levels of total protein, albumin, glucose, triglyceride, cholesterol, HDL, LDL and creatinine (p ≤ 0.05). The hepatic enzymes serum levels were not affected in AFs-treated groups but lactate dehydrogenase (LDH) concentration increased by the two AFs sources. The relative weight of the liver and spleen was increased in AFs-fed groups significantly (p ≤ 0.05). Histopathological liver examination showed the vacuolar degeneration with small and large lipid droplets in hepatocytes in the AFs- contaminated groups. Dietary AFs resulted in a significant decrease of villus height, villus width and villus surface area of the small intestine compared with the control group (p ≤ 0.05). In conclusion, the results showed that the induction of experimental aflatoxicosis via two investigated AFs sources has slight differences concerning the studied parameters so that, it seems the group consumed ground rice grains indicated slightly fewer aflatoxicosis symptoms than the methanolic extract.

Keywords



Articles in Press, Accepted Manuscript
Available Online from 14 June 2021
  • Receive Date: 02 March 2019
  • Revise Date: 08 December 2019
  • Accept Date: 08 January 2020