Protective effects of Equisetum arvense methanolic extract on testicular tissue disorders in streptozotocin-induced diabetic mice

Document Type : Original Article


1 1Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran



Diabetes in a long period can damage the testicular tissue and impair the male fertility potential. Recently, different herbal treatments have been used for the prevention of type I diabetes and its pathologic effects. Methanolic extract of Equisetum arvense has antioxidant and hypoglycemic properties. Thus, the current study aimed to evaluate the protective effects of Equisetum arvense methanolic extract (EE) on diabetes-induced detrimental effects in testicular tissue. Thirty-two adult male mice were randomly divided into four groups including control-sham, diabetic (induced by streptozotocin, 50 mg/kg for 5 days), diabetic+EE250 (250mg/kg) and diabetic+EE500 (500 mg/kg). After 45 days, all animals were euthanized and their testicles were dissected out and undergone histological analyses. Moreover, the serum level of testosterone was evaluated. Analyses showed that seminiferous tubules diameter, Leydig cells number/mm2 of the connective tissue, Sertoli cells number/tubule, serum level of testosterone and percentage of seminiferous tubules with positive tubular differentiation, repopulation and spermiogenesis indices were significantly decreased in the diabetic group in comparison with control-sham group (p < 0.05). The administration of EE in test groups significantly decreased the adverse effects of diabetes (especially D+EE500). The results of this study revealed that diabetes disturbs spermatogenesis and spermiogenesis processes. Meanwhile, the EE prevents diabetes-induced damages in testicular tissue, which may be associated with hypoglycemic and antioxidative activities in this plant.


Articles in Press, Accepted Manuscript
Available Online from 14 June 2021
  • Receive Date: 25 May 2019
  • Revise Date: 15 January 2020
  • Accept Date: 12 February 2020