Histomorphometric Evaluation of Extensor Digitorum Longus Muscle in Sciatic Nerve Regeneration Process by Tissue Engineering in Rats

Document Type : Original Article


1 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Histology and Embryology, School of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

4 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

5 Department of Chemical Engineering, Sahand University of Technology, Tabriz, Iran



Skeletal muscle atrophy induced by denervation is one of the common disorders in traumatic nerve injuries. The aim of this study was the evaluation of histomorphometrical changes of extensor digitorum longus muscle after denervation and its regeneration by tissue engineering. 90 adult male Wistar rats were randomly divided into six main groups (n=15) in three time periods (2, 4 and 8 weeks, n=5). Control group, treated without surgery, in transection (Tr) group left sciatic nerve transected, in scaffold (S) group only collagen gel scaffold was used and in the mast cell (MC) group mast cells were used, mesenchymal stem cell (MSC) group treated with mesenchymal stem cells, and in MC+MSC group, mast cell along with mesenchymal stem cell were used. In the cellular groups, the scaffold and cells were mixed and placed in the transected nerve gap. The average diameter of muscle fibers, ratio of the muscle fiber’s nuclei to the fibrocyte nuclei (mn/fn), ratio of the muscle fibers nuclei number to the muscle fiber’s number (mn/mf), the average ratio of blood vessels to muscle fibers number (v/mf) and muscles weight in Tr group was the lowest compared to the other groups, but in cellular and S groups amelioration was observed according to the time period. However, in MC+MSC group there were the highest ameliorative results. This study revealed that simultaneous use of MCs and MSCs mixed with collagen gel scaffold can be considered as a suitable approach to improving denervated skeletal muscle atrophy in patients with sciatic nerve injury.


Articles in Press, Accepted Manuscript
Available Online from 14 June 2021
  • Receive Date: 09 June 2019
  • Revise Date: 07 August 2019
  • Accept Date: 17 September 2019