Document Type : Original Article
Authors
- Mohadeseh Barazandeh 1, 2
- Khashayar Shahin 1, 2
- Abolghasem Hedayatkhah 3, 4
- Majid Komijani 5
- Mojtaba Mansoorianfar 6, 7
1 State Key Laboratory Cultivation Base of MOST, Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing, China
2 Key Laboratory of Phage Research, International Phage Research Center, Jiangsu Academy of Agricultural Sciences, Nanjing, China
3 Department of Marine Microbiology and Biogeochemistry, Royal Netherlands Institute for Sea Research and Utrecht University, Den Burg, The Netherlands
4 Department of Freshwater and Marine Ecology, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands
5 Department of Biology, Faculty of Science, Arak University, Arak, Iran
6 Department of Materials Science and Engineering, Sharif University of Technology, Tehran, Iran
7 Chinese Academy of Science, Suzhou Institute of Nano-Tech and Nano-Bionics, Suzhou, China
Abstract
Global spread and emergence of the extensively drug-resistant (XDR) strains of P. aeruginosa have become a concern, thus, searching for new alternative treatment approaches are required. This study was aimed to isolate and characterize a novel lytic phage against P. aeruginosa. Seventy XDR isolates of P. aeruginosa were collected from May to September 2018. Wastewater samples were used for isolation of lytic phage against XDR P. aeruginosa isolates. Host range, thermal and pH stability, adsorption rate, latent period, burst size and morphology of phage were determined following the standard protocols. Morphological characteristics of the phage revealed that it belonged to Podoviridae family and it was named vB-PaeP-007. Although the phage had a narrow host range, 47 out of 70 XDR isolates were susceptible to it. The adsorption rate, latent period and burst size of vB-PaeP-007 were approximately 89.80% in 8 min, 10 min and 93 phages per cell, respectively. Its lysis activity remained at a wide range of pH (4 up to 12) and temperature (– 20.00 up to 70.00 ˚C). Regarding the physiological features and host range of the vB-PaeP-007 phage, it could be a promising candidate for phage therapy and bio-controlling of infections from XDR isolates of P. aeruginosa in human and livestock storage centers.
Keywords
- Moradali MF, Ghods S, Rehm BHA. Pseudomonas aeruginosa lifestyle: a paradigm for adaptation, survival, and persistence. Front Cell Infect Microbiol 2017; 7:39 doi: 10.3389/fcimb.2017.00039.
- Komijani M, Shahin K, Barazandeh M, et al. Prevalence of extended-spectrum β-lactamases genes in clinical isolates of Pseudomonas aeruginosa. Med Lab J 2018; 12(5):34-41.
- Zaman SB, Hussain MA, Nye R, et al. A review on antibiotic resistance: alarm bells are ringing. Cureus 2017; 9(6): e1403. doi: 10.7759/cureus.1403.
- Ríos P, Rocha C, Castro W, et al. Extensively drug-resistant (XDR) Pseudomonas aeruginosa identified in Lima, Peru co-expressing a VIM-2 metallo-β-lactamase, OXA-1 β-lactamase and GES-1 extended-spectrum β-lactamase. JMM case reports 2018; 5(7): e005154. doi: 10.1099/jmmcr.0.005154.
- Magiorakos A-P, Srinivasan A, Carey RB, et al. Multidrug‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clin Microbiol Infect 2012; 18(3): 268-281.
- WHO: Guidelines for the prevention and control of carbapenem-resistant Enterobacteriaceae, Acineto-bacter baumannii and Pseudomonas aeruginosa in health care facilities. 2017:
- WHO: WHO publishes list of bacteria for which new antibiotics are urgently needed. 2017. Available at: https://www.who.int/news/item/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed. Accessed 10 June, 2021.
- Komijani M, Bouzari M, Rahimi F. Detection and characterization of a novel lytic bacteriophage (vB-KpneM-Isf48) against Klebsiella pneumoniae isolates from infected wounds carrying antibiotic-resistance genes (TEM, SHV and CTX-M). Iran Red Crescent Med J 2017; 19(2): e34475. doi:10.5812/ircmj.34475.
- Kakasisa A, Panitsa G. Bacteriophage therapy as an alternative treatment for human infections. A comprehensive review. Int J Antimicrob Agents 2019; 53(1): 16-21.
- Patel JB, Weinstein, MP, Eliopoulos, GM, et al. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute. 27th Wayne. 2017; 42-44.
- Shahin K, Bouzari M, Wang R. Isolation, characterization and genomic analysis of a novel lytic bacteriophage vB_SsoS-ISF002 infecting Shigella sonnei and Shigella flexneri. J Med Microbiol 2018: 67(3): 376-386.
- Yazdi M, Bouzari M, Ghaemi EA. Isolation and characterization of a potentially novel Siphoviridae phage (vB_SsapS-104) with lytic activity against Staphylococcus saprophyticus isolated from urinary tract infection. Folia Microbiol 2019; 64(3): 283-294.
- Sambrook J, Russell DW. Molecular cloning: a laboratory manual. Vol 2. 3rd New York, USA: Cold Spring Harbor Laboratory Press 2001; 2.25-2.39.
- Kropinski AM, Mazzocco A, Waddell TE, et al. Enumeration of bacteriophages by double agar overlay plaque assay. Methods Mol Biol 2009; 501: 69-76.
- Shahin K, Bao H, Komijani M, et al. Isolation, characterization, and PCR-based molecular identification of a siphoviridae phage infecting Shigella dysenteriae. Microb Pathog 2019; 131: 175-180
- Kropinski AM, Prangishvili D, Lavigne R. Position paper: the creation of a rational scheme for the nomenclature of viruses of Bacteria and Archaea. Environ Microbiol 2009; 11(11):2775-2777.
- Strateva T, Yordanov D. Pseudomonas aeruginosa - a phenomenon of bacterial resistance. J Med Microbiol 2009; 58(Pt 9): 1133-1148.
- Yayan J, Ghebremedhin B, Rasche K. Antibiotic resistance of Pseudomonas aeruginosa in pneumonia at a single university hospital center in Germany over a 10-year period. PloS One 2015; 10: e0139836. doi: 10.1371/journal.pone.0139836.
- Cha K, Oh HK, Jang JY, et al.Characterization of two novel bacteriophages infecting multidrug-resistant (MDR) Acinetobacter baumannii and evaluation of their therapeutic efficacy in vivo. Front Microbiol 2018; 9:696. doi: 10.3389/fmicb.2018.00696.
- Fong SA, Drilling A, Morales S, et al. Activity of bacteriophages in removing biofilms of Pseudomonas aeruginosa isolates from chronic rhinosinusitis patients. Front Cell Infect Microbiol 2017; 7:418. doi: 10.3389/fcimb.2017.00418.
- Miyata R, Yamaguchi K, Uchiyama J, et al. Characterization of a novel Pseudomonas aeruginosa bacteriophage, KPP25, of the family Podoviridae. Virus Res 2014; 189: 43-46.
- Yamaguchi K, Miyata R, Shigehisa R, et al. Genome analysis of Pseudomonas aeruginosa bacteriophage KPP23, belonging to the family Siphoviridae. Genome Announc 2014; 2(3): e00233-14. doi: 10.1128/ genomeA.00233-14.
- Yu S, Huang H, Hao Y, et al. Complete genome sequence of the myoviral bacteriophage YS35, which causes the lysis of a multidrug-resistant Pseudomonas aeruginosa Genome Announc 2018; 6(6): e01395-17. doi: 10.1128/genomeA.01395-17.
- Monson R, Foulds I, Foweraker J, et al.The Pseudo-monas aeruginosa generalized transducing phage phiPA3 is a new member of the phiKZ-like group of ‘jumbo’ phages, and infects model laboratory strains and clinical isolates from cystic fibrosis patients. Microbiology (Reading) 2011; 157(Pt 3):859-867.
- Jamal M, Andleeb S, Jalil F, et al. Isolation and characterization of a bacteriophage and its utilization against multi-drug resistant Pseudomonas aeruginosa-2995. Life Sci 2017; 190: 21-28.
- Latz S, Krüttgen A, Häfner H, et al. Differential effect of newly isolated phages belonging to PB1-like, phiKZ-like and LUZ24-like viruses against multi-drug resistant aeruginosa under varying growth conditions. Viruses 2017;9(11): 315. doi: 10.3390/ v9110315.
- Danis-Wlodarczyk K, Olszak T, Arabski M, et al. Characterization of the newly isolated lytic bacterio-phages KTN6 and KT28 and their efficacy against Pseudomonas aeruginosa PloS One 2015; 10(5): e0127603. doi: 10.1371/journal.pone.0127603.
- Gethin The significance of surface pH in chronic wounds. Wounds UK 2007;3(3): 52-56.
- Shahin K, Bouzari M. Bacteriophage application for biocontrolling Shigella flexneri in contaminated foods. J Food Sci Technol. 2018; 55(2): 550-
- Garbe J, Wesche A, Bunk B, et al. Characterization of JG024, a pseudomonas aeruginosa PB1-like broad host range phage under simulated infection conditions. BMC Microbiol 2010;10: 301. doi: 10.1186/1471-2180-10-301.
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